Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0297 and Dsui_0298 are separated by 64 nucleotides Dsui_0298 and Dsui_0299 are separated by 84 nucleotides Dsui_0299 and Dsui_0300 are separated by 4 nucleotides
Dsui_0297: Dsui_0297 - sugar fermentation stimulation protein, at 298,759 to 299,478
_0297
Dsui_0298: Dsui_0298 - Kef-type K+ ransport system, predicted NAD-binding component, at 299,543 to 300,421
_0298
Dsui_0299: Dsui_0299 - acetyltransferase, at 300,506 to 300,991
_0299
Dsui_0300: Dsui_0300 - response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain, at 300,996 to 302,621
_0300
Position (kb) 299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 298.830 kb on - strand at 298.860 kb on + strand, within Dsui_0297 at 298.860 kb on + strand at 298.938 kb on - strand, within Dsui_0297 at 298.960 kb on - strand, within Dsui_0297 at 298.960 kb on - strand, within Dsui_0297 at 298.986 kb on + strand at 298.991 kb on - strand, within Dsui_0297 at 299.249 kb on + strand, within Dsui_0297 at 299.284 kb on + strand, within Dsui_0297 at 299.346 kb on - strand at 299.369 kb on + strand at 299.409 kb on + strand at 299.464 kb on - strand at 299.554 kb on - strand at 299.715 kb on - strand, within Dsui_0298 at 299.772 kb on + strand, within Dsui_0298 at 299.796 kb on - strand, within Dsui_0298 at 299.890 kb on - strand, within Dsui_0298 at 299.896 kb on - strand, within Dsui_0298 at 299.960 kb on - strand, within Dsui_0298 at 299.980 kb on + strand at 299.999 kb on - strand, within Dsui_0298 at 300.001 kb on + strand, within Dsui_0298 at 300.001 kb on + strand, within Dsui_0298 at 300.027 kb on - strand, within Dsui_0298 at 300.098 kb on - strand, within Dsui_0298 at 300.149 kb on + strand, within Dsui_0298 at 300.149 kb on + strand at 300.152 kb on - strand, within Dsui_0298 at 300.174 kb on + strand at 300.182 kb on - strand, within Dsui_0298 at 300.237 kb on - strand, within Dsui_0298 at 300.243 kb on - strand, within Dsui_0298 at 300.272 kb on + strand, within Dsui_0298 at 300.525 kb on + strand at 300.533 kb on - strand at 300.564 kb on - strand, within Dsui_0299 at 300.616 kb on + strand at 300.616 kb on + strand at 300.663 kb on + strand at 300.671 kb on - strand, within Dsui_0299 at 300.671 kb on - strand, within Dsui_0299 at 300.677 kb on + strand at 300.734 kb on + strand, within Dsui_0299 at 300.800 kb on - strand, within Dsui_0299 at 300.812 kb on - strand, within Dsui_0299 at 300.933 kb on - strand, within Dsui_0299 at 300.961 kb on + strand at 301.046 kb on - strand at 301.124 kb on + strand at 301.132 kb on - strand at 301.132 kb on - strand at 301.241 kb on + strand, within Dsui_0300 at 301.285 kb on + strand, within Dsui_0300 at 301.303 kb on - strand, within Dsui_0300 at 301.353 kb on - strand, within Dsui_0300 at 301.374 kb on + strand, within Dsui_0300 at 301.387 kb on + strand, within Dsui_0300
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 298,830 - +0.5 298,860 + Dsui_0297 0.14 -1.5 298,860 + +2.1 298,938 - Dsui_0297 0.25 -0.5 298,960 - Dsui_0297 0.28 -0.5 298,960 - Dsui_0297 0.28 -1.8 298,986 + +0.5 298,991 - Dsui_0297 0.32 -3.0 299,249 + Dsui_0297 0.68 -1.1 299,284 + Dsui_0297 0.73 -1.1 299,346 - +0.5 299,369 + -0.5 299,409 + +0.5 299,464 - -0.5 299,554 - -0.5 299,715 - Dsui_0298 0.20 +1.3 299,772 + Dsui_0298 0.26 -1.1 299,796 - Dsui_0298 0.29 -0.7 299,890 - Dsui_0298 0.39 -0.2 299,896 - Dsui_0298 0.40 -3.2 299,960 - Dsui_0298 0.47 -0.1 299,980 + +0.5 299,999 - Dsui_0298 0.52 +1.6 300,001 + Dsui_0298 0.52 +0.5 300,001 + Dsui_0298 0.52 +0.5 300,027 - Dsui_0298 0.55 -3.3 300,098 - Dsui_0298 0.63 +0.1 300,149 + Dsui_0298 0.69 -2.3 300,149 + +0.5 300,152 - Dsui_0298 0.69 -1.6 300,174 + +0.5 300,182 - Dsui_0298 0.73 +0.6 300,237 - Dsui_0298 0.79 -0.4 300,243 - Dsui_0298 0.80 +0.5 300,272 + Dsui_0298 0.83 -1.1 300,525 + -1.5 300,533 - -0.9 300,564 - Dsui_0299 0.12 -0.4 300,616 + -1.1 300,616 + +0.5 300,663 + +0.5 300,671 - Dsui_0299 0.34 -0.4 300,671 - Dsui_0299 0.34 -0.6 300,677 + -0.5 300,734 + Dsui_0299 0.47 -1.5 300,800 - Dsui_0299 0.60 -0.3 300,812 - Dsui_0299 0.63 -1.0 300,933 - Dsui_0299 0.88 -0.0 300,961 + -0.5 301,046 - -0.0 301,124 + +0.5 301,132 - -0.3 301,132 - -0.4 301,241 + Dsui_0300 0.15 +0.5 301,285 + Dsui_0300 0.18 +0.5 301,303 - Dsui_0300 0.19 -0.2 301,353 - Dsui_0300 0.22 -0.1 301,374 + Dsui_0300 0.23 -1.8 301,387 + Dsui_0300 0.24 -1.5
Or see this region's nucleotide sequence