Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0026 and Dsui_0027 are separated by 214 nucleotides Dsui_0027 and Dsui_0028 overlap by 4 nucleotides Dsui_0028 and Dsui_0029 are separated by 83 nucleotides Dsui_0029 and Dsui_0030 are separated by 141 nucleotides
Dsui_0026: Dsui_0026 - CBS domain-containing protein, at 23,840 to 25,126
_0026
Dsui_0027: Dsui_0027 - putative effector of murein hydrolase LrgA, at 25,341 to 25,703
_0027
Dsui_0028: Dsui_0028 - putative effector of murein hydrolase, at 25,700 to 26,455
_0028
Dsui_0029: Dsui_0029 - putative pyrophosphatase, at 26,539 to 26,886
_0029
Dsui_0030: Dsui_0030 - FtsH-interacting integral membrane protein, at 27,028 to 27,720
_0030
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.706 kb on + strand, within Dsui_0026 at 24.741 kb on + strand at 24.749 kb on - strand, within Dsui_0026 at 24.817 kb on + strand at 24.823 kb on + strand, within Dsui_0026 at 24.831 kb on - strand, within Dsui_0026 at 24.853 kb on + strand at 24.861 kb on - strand, within Dsui_0026 at 24.918 kb on - strand, within Dsui_0026 at 24.976 kb on - strand, within Dsui_0026 at 25.099 kb on - strand at 25.190 kb on - strand at 25.233 kb on - strand at 25.287 kb on - strand at 25.355 kb on + strand at 25.396 kb on + strand at 25.480 kb on + strand at 25.498 kb on - strand at 25.596 kb on + strand, within Dsui_0027 at 25.660 kb on - strand, within Dsui_0027 at 25.667 kb on - strand at 25.667 kb on - strand at 25.739 kb on + strand at 25.741 kb on - strand at 25.777 kb on - strand, within Dsui_0028 at 25.785 kb on + strand, within Dsui_0028 at 25.793 kb on - strand at 25.828 kb on - strand at 25.979 kb on + strand, within Dsui_0028 at 26.007 kb on - strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.008 kb on + strand, within Dsui_0028 at 26.016 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.028 kb on - strand, within Dsui_0028 at 26.044 kb on + strand at 26.235 kb on - strand, within Dsui_0028 at 26.262 kb on + strand, within Dsui_0028 at 26.275 kb on + strand at 26.345 kb on - strand, within Dsui_0028 at 26.377 kb on + strand, within Dsui_0028 at 26.386 kb on + strand at 26.434 kb on + strand at 26.538 kb on + strand at 26.545 kb on - strand at 26.567 kb on - strand at 26.574 kb on - strand, within Dsui_0029 at 26.665 kb on - strand, within Dsui_0029 at 26.671 kb on + strand, within Dsui_0029 at 26.724 kb on + strand, within Dsui_0029 at 26.732 kb on - strand, within Dsui_0029 at 26.785 kb on - strand at 26.810 kb on + strand, within Dsui_0029 at 26.825 kb on + strand, within Dsui_0029 at 26.825 kb on + strand, within Dsui_0029 at 27.046 kb on + strand at 27.054 kb on - strand at 27.079 kb on + strand at 27.085 kb on + strand at 27.087 kb on - strand at 27.087 kb on - strand at 27.110 kb on + strand, within Dsui_0030 at 27.150 kb on + strand, within Dsui_0030 at 27.306 kb on + strand at 27.343 kb on + strand at 27.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 24,706 + Dsui_0026 0.67 -0.5 24,741 + +0.5 24,749 - Dsui_0026 0.71 -1.1 24,817 + +0.5 24,823 + Dsui_0026 0.76 -2.3 24,831 - Dsui_0026 0.77 -0.5 24,853 + +0.5 24,861 - Dsui_0026 0.79 +0.5 24,918 - Dsui_0026 0.84 -2.3 24,976 - Dsui_0026 0.88 +0.5 25,099 - +0.5 25,190 - +0.5 25,233 - -0.5 25,287 - -0.5 25,355 + -0.5 25,396 + +0.5 25,480 + +0.5 25,498 - -1.1 25,596 + Dsui_0027 0.70 +0.5 25,660 - Dsui_0027 0.88 +0.5 25,667 - +0.5 25,667 - -0.5 25,739 + +0.5 25,741 - -1.1 25,777 - Dsui_0028 0.10 -1.8 25,785 + Dsui_0028 0.11 -0.5 25,793 - +0.5 25,828 - +0.5 25,979 + Dsui_0028 0.37 +0.5 26,007 - Dsui_0028 0.41 +0.5 26,008 + Dsui_0028 0.41 -0.5 26,008 + Dsui_0028 0.41 -2.1 26,016 - Dsui_0028 0.42 -1.1 26,028 - Dsui_0028 0.43 +0.5 26,028 - Dsui_0028 0.43 -1.1 26,044 + -0.5 26,235 - Dsui_0028 0.71 -1.1 26,262 + Dsui_0028 0.74 -1.1 26,275 + +0.5 26,345 - Dsui_0028 0.85 +0.5 26,377 + Dsui_0028 0.90 -2.1 26,386 + +0.5 26,434 + -1.1 26,538 + +0.5 26,545 - -0.5 26,567 - -0.5 26,574 - Dsui_0029 0.10 -0.5 26,665 - Dsui_0029 0.36 +0.5 26,671 + Dsui_0029 0.38 -0.5 26,724 + Dsui_0029 0.53 -1.5 26,732 - Dsui_0029 0.55 -0.5 26,785 - +0.5 26,810 + Dsui_0029 0.78 +0.5 26,825 + Dsui_0029 0.82 -1.1 26,825 + Dsui_0029 0.82 -0.5 27,046 + +0.5 27,054 - +0.5 27,079 + +0.5 27,085 + +0.5 27,087 - +0.5 27,087 - -1.8 27,110 + Dsui_0030 0.12 -0.5 27,150 + Dsui_0030 0.18 -2.5 27,306 + +0.5 27,343 + -1.1 27,385 + +0.5
Or see this region's nucleotide sequence