Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23915

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23900 and BWI76_RS23905 are separated by 159 nucleotidesBWI76_RS23905 and BWI76_RS23915 are separated by 327 nucleotidesBWI76_RS23915 and BWI76_RS23920 are separated by 377 nucleotides BWI76_RS23900: BWI76_RS23900 - hypothetical protein, at 4,989,149 to 4,989,733 _RS23900 BWI76_RS23905: BWI76_RS23905 - Z-ring-associated protein, at 4,989,893 to 4,990,222 _RS23905 BWI76_RS23915: BWI76_RS23915 - 5-formyltetrahydrofolate cyclo-ligase, at 4,990,550 to 4,991,146 _RS23915 BWI76_RS23920: BWI76_RS23920 - D-3-phosphoglycerate dehydrogenase, at 4,991,524 to 4,992,756 _RS23920 Position (kb) 4990 4991 4992Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4989.661 kb on - strand, within BWI76_RS23900at 4989.726 kb on - strandat 4989.726 kb on - strandat 4989.780 kb on - strandat 4989.815 kb on - strandat 4989.828 kb on + strandat 4989.964 kb on - strand, within BWI76_RS23905at 4989.968 kb on + strand, within BWI76_RS23905at 4989.969 kb on + strand, within BWI76_RS23905at 4989.969 kb on + strand, within BWI76_RS23905at 4989.976 kb on - strand, within BWI76_RS23905at 4990.133 kb on - strand, within BWI76_RS23905at 4990.154 kb on - strand, within BWI76_RS23905at 4990.158 kb on + strand, within BWI76_RS23905at 4990.239 kb on + strandat 4990.247 kb on - strandat 4990.770 kb on - strand, within BWI76_RS23915at 4991.082 kb on + strand, within BWI76_RS23915at 4991.197 kb on + strandat 4991.198 kb on + strandat 4991.205 kb on - strandat 4991.354 kb on - strandat 4991.744 kb on + strand, within BWI76_RS23920at 4991.783 kb on + strand, within BWI76_RS23920at 4991.801 kb on - strand, within BWI76_RS23920at 4991.924 kb on - strand, within BWI76_RS23920

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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4,989,661 - BWI76_RS23900 0.88 +0.1
4,989,726 - +0.8
4,989,726 - -0.4
4,989,780 - -0.3
4,989,815 - -0.6
4,989,828 + -0.2
4,989,964 - BWI76_RS23905 0.22 -0.2
4,989,968 + BWI76_RS23905 0.23 -0.3
4,989,969 + BWI76_RS23905 0.23 +0.1
4,989,969 + BWI76_RS23905 0.23 -0.3
4,989,976 - BWI76_RS23905 0.25 -1.2
4,990,133 - BWI76_RS23905 0.73 +0.5
4,990,154 - BWI76_RS23905 0.79 +0.1
4,990,158 + BWI76_RS23905 0.80 -0.6
4,990,239 + +1.0
4,990,247 - -0.1
4,990,770 - BWI76_RS23915 0.37 -1.3
4,991,082 + BWI76_RS23915 0.89 -0.2
4,991,197 + -1.3
4,991,198 + +0.1
4,991,205 - -1.4
4,991,354 - +1.1
4,991,744 + BWI76_RS23920 0.18 -2.7
4,991,783 + BWI76_RS23920 0.21 -4.3
4,991,801 - BWI76_RS23920 0.22 -6.0
4,991,924 - BWI76_RS23920 0.32 -2.6

Or see this region's nucleotide sequence