Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35990 and RR42_RS35995 are separated by 38 nucleotides RR42_RS35995 and RR42_RS36000 are separated by 61 nucleotides
RR42_RS35990: RR42_RS35990 - pilus assembly protein, at 3,522,390 to 3,523,607
_RS35990
RR42_RS35995: RR42_RS35995 - membrane protein, at 3,523,646 to 3,525,043
_RS35995
RR42_RS36000: RR42_RS36000 - pilus assembly protein CpaB, at 3,525,105 to 3,526,076
_RS36000
Position (kb)
3523
3524
3525
3526 Strain fitness (log2 ratio)
-2
-1
0
1 at 3522.845 kb on + strand, within RR42_RS35990 at 3522.845 kb on + strand, within RR42_RS35990 at 3522.846 kb on - strand, within RR42_RS35990 at 3523.075 kb on + strand, within RR42_RS35990 at 3523.076 kb on - strand, within RR42_RS35990 at 3523.393 kb on + strand, within RR42_RS35990 at 3523.586 kb on + strand at 3523.586 kb on + strand at 3523.587 kb on - strand at 3523.681 kb on - strand at 3523.695 kb on + strand at 3523.695 kb on + strand at 3523.695 kb on + strand at 3523.695 kb on + strand at 3523.696 kb on - strand at 3523.696 kb on - strand at 3523.696 kb on - strand at 3523.696 kb on - strand at 3523.696 kb on - strand at 3523.696 kb on - strand at 3523.853 kb on - strand, within RR42_RS35995 at 3524.015 kb on + strand, within RR42_RS35995 at 3524.016 kb on - strand, within RR42_RS35995 at 3524.814 kb on - strand, within RR42_RS35995 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.572 kb on - strand, within RR42_RS36000 at 3525.844 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.877 kb on - strand, within RR42_RS36000 at 3525.943 kb on + strand, within RR42_RS36000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 3,522,845 + RR42_RS35990 0.37 +0.4 3,522,845 + RR42_RS35990 0.37 -1.4 3,522,846 - RR42_RS35990 0.37 -0.5 3,523,075 + RR42_RS35990 0.56 -0.5 3,523,076 - RR42_RS35990 0.56 +0.6 3,523,393 + RR42_RS35990 0.82 +0.9 3,523,586 + -0.7 3,523,586 + +0.1 3,523,587 - -0.3 3,523,681 - +0.7 3,523,695 + +1.0 3,523,695 + -0.3 3,523,695 + +1.2 3,523,695 + +0.5 3,523,696 - -2.5 3,523,696 - -2.2 3,523,696 - -1.1 3,523,696 - +1.3 3,523,696 - -1.2 3,523,696 - +0.7 3,523,853 - RR42_RS35995 0.15 -0.7 3,524,015 + RR42_RS35995 0.26 -1.9 3,524,016 - RR42_RS35995 0.26 -0.8 3,524,814 - RR42_RS35995 0.84 -0.1 3,525,357 + RR42_RS36000 0.26 +0.3 3,525,357 + RR42_RS36000 0.26 -0.6 3,525,572 - RR42_RS36000 0.48 +0.3 3,525,844 + RR42_RS36000 0.76 +0.6 3,525,867 + RR42_RS36000 0.78 +0.6 3,525,867 + RR42_RS36000 0.78 -0.7 3,525,877 - RR42_RS36000 0.79 -0.3 3,525,943 + RR42_RS36000 0.86 +0.3
Or see this region's nucleotide sequence