Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27205 and RR42_RS27210 overlap by 4 nucleotides RR42_RS27210 and RR42_RS27215 are separated by 93 nucleotides RR42_RS27215 and RR42_RS27220 are separated by 184 nucleotides
RR42_RS27205: RR42_RS27205 - GlcNAc-PI de-N-acetylase, at 1,455,749 to 1,456,498
_RS27205
RR42_RS27210: RR42_RS27210 - hypothetical protein, at 1,456,495 to 1,457,208
_RS27210
RR42_RS27215: RR42_RS27215 - LysR family transcriptional regulator, at 1,457,302 to 1,458,252
_RS27215
RR42_RS27220: RR42_RS27220 - tricarballylate dehydrogenase, at 1,458,437 to 1,459,864
_RS27220
Position (kb)
1457
1458
1459 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1456.309 kb on + strand, within RR42_RS27205 at 1456.310 kb on - strand, within RR42_RS27205 at 1456.379 kb on + strand, within RR42_RS27205 at 1456.382 kb on + strand, within RR42_RS27205 at 1456.382 kb on + strand, within RR42_RS27205 at 1456.446 kb on - strand at 1456.457 kb on + strand at 1456.457 kb on + strand at 1456.458 kb on - strand at 1456.616 kb on - strand, within RR42_RS27210 at 1457.122 kb on + strand, within RR42_RS27210 at 1457.123 kb on - strand, within RR42_RS27210 at 1457.123 kb on - strand, within RR42_RS27210 at 1457.242 kb on - strand at 1457.309 kb on + strand at 1457.379 kb on + strand at 1457.664 kb on + strand, within RR42_RS27215 at 1457.665 kb on - strand, within RR42_RS27215 at 1457.665 kb on - strand, within RR42_RS27215 at 1458.147 kb on - strand, within RR42_RS27215 at 1458.311 kb on + strand at 1458.312 kb on - strand at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 1,456,309 + RR42_RS27205 0.75 -1.0 1,456,310 - RR42_RS27205 0.75 +0.9 1,456,379 + RR42_RS27205 0.84 -0.6 1,456,382 + RR42_RS27205 0.84 +1.2 1,456,382 + RR42_RS27205 0.84 -0.2 1,456,446 - -0.1 1,456,457 + -2.2 1,456,457 + -2.9 1,456,458 - -0.7 1,456,616 - RR42_RS27210 0.17 +1.2 1,457,122 + RR42_RS27210 0.88 -0.8 1,457,123 - RR42_RS27210 0.88 -1.0 1,457,123 - RR42_RS27210 0.88 -1.3 1,457,242 - -0.7 1,457,309 + -0.1 1,457,379 + -0.1 1,457,664 + RR42_RS27215 0.38 -0.2 1,457,665 - RR42_RS27215 0.38 -0.7 1,457,665 - RR42_RS27215 0.38 -1.7 1,458,147 - RR42_RS27215 0.89 -0.8 1,458,311 + +0.9 1,458,312 - +0.6 1,458,876 - RR42_RS27220 0.31 -2.6 1,458,876 - RR42_RS27220 0.31 -0.5 1,458,876 - RR42_RS27220 0.31 -0.1 1,459,181 + RR42_RS27220 0.52 +0.4 1,459,181 + RR42_RS27220 0.52 +0.2 1,459,181 + RR42_RS27220 0.52 -0.8
Or see this region's nucleotide sequence