Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25465

Experiment: R2A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25455 and RR42_RS25460 are separated by 128 nucleotidesRR42_RS25460 and RR42_RS25465 are separated by 5 nucleotidesRR42_RS25465 and RR42_RS25470 are separated by 32 nucleotidesRR42_RS25470 and RR42_RS25475 overlap by 4 nucleotides RR42_RS25455: RR42_RS25455 - acetyl-CoA acetyltransferase, at 1,057,470 to 1,058,654 _RS25455 RR42_RS25460: RR42_RS25460 - IclR family transcriptional regulator, at 1,058,783 to 1,059,631 _RS25460 RR42_RS25465: RR42_RS25465 - long-chain fatty acid--CoA ligase, at 1,059,637 to 1,061,289 _RS25465 RR42_RS25470: RR42_RS25470 - alpha/beta hydrolase, at 1,061,322 to 1,062,113 _RS25470 RR42_RS25475: RR42_RS25475 - peptidase S15, at 1,062,110 to 1,063,024 _RS25475 Position (kb) 1059 1060 1061 1062Strain fitness (log2 ratio) -2 -1 0 1at 1058.784 kb on + strandat 1058.785 kb on - strandat 1058.880 kb on - strand, within RR42_RS25460at 1059.119 kb on + strand, within RR42_RS25460at 1059.184 kb on + strand, within RR42_RS25460at 1059.217 kb on + strand, within RR42_RS25460at 1059.217 kb on + strand, within RR42_RS25460at 1059.219 kb on + strand, within RR42_RS25460at 1059.220 kb on - strand, within RR42_RS25460at 1059.220 kb on - strand, within RR42_RS25460at 1059.220 kb on - strand, within RR42_RS25460at 1059.418 kb on + strand, within RR42_RS25460at 1059.418 kb on + strand, within RR42_RS25460at 1059.419 kb on - strand, within RR42_RS25460at 1059.419 kb on - strand, within RR42_RS25460at 1059.420 kb on + strand, within RR42_RS25460at 1059.421 kb on - strand, within RR42_RS25460at 1059.663 kb on - strandat 1059.676 kb on - strandat 1059.689 kb on + strandat 1059.689 kb on + strandat 1059.730 kb on + strandat 1059.730 kb on + strandat 1059.749 kb on + strandat 1059.750 kb on - strandat 1059.750 kb on - strandat 1059.750 kb on - strandat 1059.777 kb on - strandat 1059.908 kb on + strand, within RR42_RS25465at 1059.908 kb on + strand, within RR42_RS25465at 1059.908 kb on + strand, within RR42_RS25465at 1059.909 kb on - strand, within RR42_RS25465at 1060.023 kb on + strand, within RR42_RS25465at 1060.024 kb on - strand, within RR42_RS25465at 1060.030 kb on - strand, within RR42_RS25465at 1060.038 kb on + strand, within RR42_RS25465at 1060.038 kb on + strand, within RR42_RS25465at 1060.039 kb on - strand, within RR42_RS25465at 1060.137 kb on + strand, within RR42_RS25465at 1060.137 kb on + strand, within RR42_RS25465at 1060.183 kb on + strand, within RR42_RS25465at 1060.183 kb on + strand, within RR42_RS25465at 1060.184 kb on - strand, within RR42_RS25465at 1060.184 kb on - strand, within RR42_RS25465at 1060.184 kb on - strand, within RR42_RS25465at 1060.184 kb on - strand, within RR42_RS25465at 1060.308 kb on + strand, within RR42_RS25465at 1060.308 kb on + strand, within RR42_RS25465at 1060.309 kb on - strand, within RR42_RS25465at 1060.518 kb on + strand, within RR42_RS25465at 1060.518 kb on + strand, within RR42_RS25465at 1060.519 kb on - strand, within RR42_RS25465at 1060.519 kb on - strand, within RR42_RS25465at 1060.519 kb on - strand, within RR42_RS25465at 1060.533 kb on - strand, within RR42_RS25465at 1060.566 kb on - strand, within RR42_RS25465at 1060.613 kb on + strand, within RR42_RS25465at 1060.680 kb on + strand, within RR42_RS25465at 1060.681 kb on - strand, within RR42_RS25465at 1060.707 kb on - strand, within RR42_RS25465at 1060.743 kb on + strand, within RR42_RS25465at 1060.745 kb on + strand, within RR42_RS25465at 1060.746 kb on - strand, within RR42_RS25465at 1060.824 kb on - strand, within RR42_RS25465at 1060.965 kb on + strand, within RR42_RS25465at 1060.966 kb on - strand, within RR42_RS25465at 1060.966 kb on - strand, within RR42_RS25465at 1060.966 kb on - strand, within RR42_RS25465at 1060.967 kb on + strand, within RR42_RS25465at 1060.968 kb on - strand, within RR42_RS25465at 1061.012 kb on - strand, within RR42_RS25465at 1061.051 kb on + strand, within RR42_RS25465at 1061.085 kb on + strand, within RR42_RS25465at 1061.157 kb on - strandat 1061.160 kb on + strandat 1061.160 kb on + strandat 1061.161 kb on - strandat 1061.161 kb on - strandat 1061.172 kb on + strandat 1061.172 kb on + strandat 1061.247 kb on - strandat 1061.247 kb on - strandat 1061.655 kb on + strand, within RR42_RS25470at 1061.844 kb on - strand, within RR42_RS25470at 1062.042 kb on + strandat 1062.047 kb on - strandat 1062.103 kb on - strandat 1062.113 kb on + strandat 1062.113 kb on + strandat 1062.145 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A
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1,058,784 + -0.1
1,058,785 - -0.6
1,058,880 - RR42_RS25460 0.11 +1.1
1,059,119 + RR42_RS25460 0.40 +0.2
1,059,184 + RR42_RS25460 0.47 -1.3
1,059,217 + RR42_RS25460 0.51 -0.6
1,059,217 + RR42_RS25460 0.51 +0.1
1,059,219 + RR42_RS25460 0.51 +0.1
1,059,220 - RR42_RS25460 0.51 -1.5
1,059,220 - RR42_RS25460 0.51 -0.0
1,059,220 - RR42_RS25460 0.51 +0.3
1,059,418 + RR42_RS25460 0.75 +0.1
1,059,418 + RR42_RS25460 0.75 -0.6
1,059,419 - RR42_RS25460 0.75 +0.3
1,059,419 - RR42_RS25460 0.75 +0.5
1,059,420 + RR42_RS25460 0.75 +0.4
1,059,421 - RR42_RS25460 0.75 -1.0
1,059,663 - +0.4
1,059,676 - -0.8
1,059,689 + +0.8
1,059,689 + -0.1
1,059,730 + -2.0
1,059,730 + +0.4
1,059,749 + -0.5
1,059,750 - -1.1
1,059,750 - -0.7
1,059,750 - -0.3
1,059,777 - -1.7
1,059,908 + RR42_RS25465 0.16 -0.5
1,059,908 + RR42_RS25465 0.16 -0.7
1,059,908 + RR42_RS25465 0.16 -0.4
1,059,909 - RR42_RS25465 0.16 -0.3
1,060,023 + RR42_RS25465 0.23 -0.1
1,060,024 - RR42_RS25465 0.23 -1.1
1,060,030 - RR42_RS25465 0.24 -1.0
1,060,038 + RR42_RS25465 0.24 +0.8
1,060,038 + RR42_RS25465 0.24 +0.0
1,060,039 - RR42_RS25465 0.24 -0.5
1,060,137 + RR42_RS25465 0.30 -1.4
1,060,137 + RR42_RS25465 0.30 +0.7
1,060,183 + RR42_RS25465 0.33 +0.3
1,060,183 + RR42_RS25465 0.33 +0.1
1,060,184 - RR42_RS25465 0.33 -0.1
1,060,184 - RR42_RS25465 0.33 -0.1
1,060,184 - RR42_RS25465 0.33 -0.3
1,060,184 - RR42_RS25465 0.33 -0.3
1,060,308 + RR42_RS25465 0.41 -0.6
1,060,308 + RR42_RS25465 0.41 +0.7
1,060,309 - RR42_RS25465 0.41 +0.4
1,060,518 + RR42_RS25465 0.53 -0.2
1,060,518 + RR42_RS25465 0.53 -0.6
1,060,519 - RR42_RS25465 0.53 -0.1
1,060,519 - RR42_RS25465 0.53 -1.3
1,060,519 - RR42_RS25465 0.53 -0.1
1,060,533 - RR42_RS25465 0.54 -0.4
1,060,566 - RR42_RS25465 0.56 -1.7
1,060,613 + RR42_RS25465 0.59 -0.3
1,060,680 + RR42_RS25465 0.63 -1.4
1,060,681 - RR42_RS25465 0.63 -2.1
1,060,707 - RR42_RS25465 0.65 -0.4
1,060,743 + RR42_RS25465 0.67 +0.6
1,060,745 + RR42_RS25465 0.67 -0.0
1,060,746 - RR42_RS25465 0.67 +0.2
1,060,824 - RR42_RS25465 0.72 +0.3
1,060,965 + RR42_RS25465 0.80 -0.9
1,060,966 - RR42_RS25465 0.80 -0.5
1,060,966 - RR42_RS25465 0.80 -0.5
1,060,966 - RR42_RS25465 0.80 -0.5
1,060,967 + RR42_RS25465 0.80 +1.0
1,060,968 - RR42_RS25465 0.81 +0.1
1,061,012 - RR42_RS25465 0.83 -0.2
1,061,051 + RR42_RS25465 0.86 -0.9
1,061,085 + RR42_RS25465 0.88 +1.1
1,061,157 - -0.2
1,061,160 + +0.2
1,061,160 + +0.0
1,061,161 - -0.1
1,061,161 - -1.0
1,061,172 + +0.6
1,061,172 + -1.3
1,061,247 - +0.1
1,061,247 - -0.1
1,061,655 + RR42_RS25470 0.42 +0.7
1,061,844 - RR42_RS25470 0.66 +0.8
1,062,042 + -0.7
1,062,047 - +0.2
1,062,103 - +1.5
1,062,113 + +0.1
1,062,113 + +0.2
1,062,145 - -0.2

Or see this region's nucleotide sequence