Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10140 and RR42_RS10145 are separated by 173 nucleotides RR42_RS10145 and RR42_RS10150 are separated by 112 nucleotides RR42_RS10150 and RR42_RS10155 overlap by 4 nucleotides
RR42_RS10140: RR42_RS10140 - 8-amino-7-oxononanoate synthase, at 2,173,619 to 2,174,842
_RS10140
RR42_RS10145: RR42_RS10145 - hypothetical protein, at 2,175,016 to 2,175,243
_RS10145
RR42_RS10150: RR42_RS10150 - hypothetical protein, at 2,175,356 to 2,175,637
_RS10150
RR42_RS10155: RR42_RS10155 - hypothetical protein, at 2,175,634 to 2,175,933
_RS10155
Position (kb)
2175
2176 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2174.022 kb on + strand, within RR42_RS10140 at 2174.023 kb on - strand, within RR42_RS10140 at 2174.026 kb on + strand, within RR42_RS10140 at 2174.026 kb on + strand, within RR42_RS10140 at 2174.026 kb on + strand, within RR42_RS10140 at 2174.028 kb on + strand, within RR42_RS10140 at 2174.029 kb on - strand, within RR42_RS10140 at 2174.124 kb on + strand, within RR42_RS10140 at 2174.288 kb on + strand, within RR42_RS10140 at 2174.289 kb on - strand, within RR42_RS10140 at 2174.378 kb on + strand, within RR42_RS10140 at 2174.379 kb on - strand, within RR42_RS10140 at 2174.493 kb on + strand, within RR42_RS10140 at 2174.530 kb on + strand, within RR42_RS10140 at 2174.531 kb on - strand, within RR42_RS10140 at 2174.625 kb on + strand, within RR42_RS10140 at 2174.685 kb on + strand, within RR42_RS10140 at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.877 kb on - strand at 2174.877 kb on - strand at 2174.877 kb on - strand at 2175.035 kb on + strand at 2175.035 kb on + strand at 2175.036 kb on - strand at 2175.036 kb on - strand at 2175.036 kb on - strand at 2175.163 kb on + strand, within RR42_RS10145 at 2175.163 kb on + strand, within RR42_RS10145 at 2175.164 kb on - strand, within RR42_RS10145 at 2175.164 kb on - strand, within RR42_RS10145 at 2175.211 kb on + strand, within RR42_RS10145 at 2175.211 kb on + strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.247 kb on - strand at 2175.247 kb on - strand at 2175.247 kb on - strand at 2175.296 kb on + strand at 2175.297 kb on - strand at 2175.297 kb on - strand at 2175.297 kb on - strand at 2175.300 kb on + strand at 2175.301 kb on - strand at 2175.301 kb on - strand at 2175.326 kb on + strand at 2175.326 kb on + strand at 2175.326 kb on + strand at 2175.327 kb on - strand at 2175.327 kb on - strand at 2175.327 kb on - strand at 2175.345 kb on + strand at 2175.345 kb on + strand at 2175.346 kb on - strand at 2175.358 kb on - strand at 2175.445 kb on - strand, within RR42_RS10150 at 2175.445 kb on - strand, within RR42_RS10150 at 2175.851 kb on + strand, within RR42_RS10155 at 2175.851 kb on + strand, within RR42_RS10155 at 2175.981 kb on + strand at 2176.021 kb on - strand at 2176.074 kb on - strand at 2176.074 kb on - strand at 2176.074 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.227 kb on + strand at 2176.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 2,174,022 + RR42_RS10140 0.33 +0.3 2,174,023 - RR42_RS10140 0.33 +0.1 2,174,026 + RR42_RS10140 0.33 -1.6 2,174,026 + RR42_RS10140 0.33 +0.1 2,174,026 + RR42_RS10140 0.33 -2.1 2,174,028 + RR42_RS10140 0.33 +0.6 2,174,029 - RR42_RS10140 0.33 +0.8 2,174,124 + RR42_RS10140 0.41 -0.4 2,174,288 + RR42_RS10140 0.55 -0.6 2,174,289 - RR42_RS10140 0.55 +0.0 2,174,378 + RR42_RS10140 0.62 +0.2 2,174,379 - RR42_RS10140 0.62 +1.2 2,174,493 + RR42_RS10140 0.71 -0.6 2,174,530 + RR42_RS10140 0.74 -1.0 2,174,531 - RR42_RS10140 0.75 +0.4 2,174,625 + RR42_RS10140 0.82 -1.1 2,174,685 + RR42_RS10140 0.87 +0.2 2,174,876 + +0.2 2,174,876 + +1.2 2,174,876 + +0.1 2,174,876 + +0.7 2,174,876 + -0.1 2,174,877 - +0.5 2,174,877 - -1.1 2,174,877 - -0.4 2,175,035 + +0.1 2,175,035 + -0.6 2,175,036 - -0.4 2,175,036 - +0.1 2,175,036 - -0.3 2,175,163 + RR42_RS10145 0.64 -1.0 2,175,163 + RR42_RS10145 0.64 +0.1 2,175,164 - RR42_RS10145 0.65 +0.4 2,175,164 - RR42_RS10145 0.65 -0.0 2,175,211 + RR42_RS10145 0.86 -1.2 2,175,211 + RR42_RS10145 0.86 -0.3 2,175,212 - RR42_RS10145 0.86 +0.3 2,175,212 - RR42_RS10145 0.86 +1.0 2,175,212 - RR42_RS10145 0.86 +0.5 2,175,212 - RR42_RS10145 0.86 +0.1 2,175,212 - RR42_RS10145 0.86 +0.2 2,175,247 - -0.3 2,175,247 - -0.1 2,175,247 - -0.6 2,175,296 + -0.6 2,175,297 - -0.4 2,175,297 - -0.1 2,175,297 - -0.2 2,175,300 + -1.3 2,175,301 - -0.3 2,175,301 - -1.4 2,175,326 + -0.9 2,175,326 + +0.3 2,175,326 + +0.6 2,175,327 - +0.2 2,175,327 - -1.3 2,175,327 - -0.1 2,175,345 + -0.1 2,175,345 + +0.2 2,175,346 - -0.8 2,175,358 - -0.8 2,175,445 - RR42_RS10150 0.32 -0.7 2,175,445 - RR42_RS10150 0.32 -0.8 2,175,851 + RR42_RS10155 0.72 -0.9 2,175,851 + RR42_RS10155 0.72 +1.0 2,175,981 + -0.7 2,176,021 - -0.2 2,176,074 - -1.2 2,176,074 - -3.4 2,176,074 - +0.4 2,176,216 - -0.5 2,176,216 - -2.0 2,176,216 - +0.2 2,176,216 - +1.1 2,176,227 + +0.4 2,176,228 - +0.7
Or see this region's nucleotide sequence