Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02955 and RR42_RS02960 overlap by 1 nucleotides RR42_RS02960 and RR42_RS02965 overlap by 1 nucleotides RR42_RS02965 and RR42_RS02970 are separated by 348 nucleotides RR42_RS02970 and RR42_RS02975 are separated by 10 nucleotides
RR42_RS02955: RR42_RS02955 - malonate decarboxylase subunit delta, at 656,850 to 657,164
_RS02955
RR42_RS02960: RR42_RS02960 - triphosphoribosyl-dephospho-CoA synthase, at 657,164 to 658,069
_RS02960
RR42_RS02965: RR42_RS02965 - malonate decarboxylase subunit alpha, at 658,069 to 659,727
_RS02965
RR42_RS02970: RR42_RS02970 - malonate carrier protein, at 660,076 to 660,486
_RS02970
RR42_RS02975: RR42_RS02975 - malonate transporter, at 660,497 to 661,273
_RS02975
Position (kb) 658
659
660 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 657.165 kb on + strand at 657.568 kb on - strand, within RR42_RS02960 at 657.568 kb on - strand, within RR42_RS02960 at 657.670 kb on + strand, within RR42_RS02960 at 657.671 kb on - strand, within RR42_RS02960 at 658.053 kb on - strand at 658.100 kb on + strand at 658.319 kb on + strand, within RR42_RS02965 at 658.358 kb on - strand, within RR42_RS02965 at 658.370 kb on + strand, within RR42_RS02965 at 658.371 kb on - strand, within RR42_RS02965 at 658.371 kb on - strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.770 kb on - strand, within RR42_RS02965 at 658.770 kb on - strand, within RR42_RS02965 at 658.869 kb on - strand, within RR42_RS02965 at 658.941 kb on + strand, within RR42_RS02965 at 658.985 kb on + strand, within RR42_RS02965 at 659.240 kb on + strand, within RR42_RS02965 at 659.306 kb on + strand, within RR42_RS02965 at 659.307 kb on - strand, within RR42_RS02965 at 659.330 kb on + strand, within RR42_RS02965 at 659.330 kb on + strand, within RR42_RS02965 at 659.330 kb on + strand, within RR42_RS02965 at 659.330 kb on + strand, within RR42_RS02965 at 659.331 kb on - strand, within RR42_RS02965 at 659.331 kb on - strand, within RR42_RS02965 at 659.815 kb on - strand at 659.825 kb on + strand at 659.826 kb on - strand at 659.880 kb on - strand at 659.919 kb on + strand at 659.948 kb on + strand at 659.949 kb on - strand at 660.005 kb on + strand at 660.006 kb on - strand at 660.006 kb on - strand at 660.026 kb on - strand at 660.085 kb on + strand at 660.085 kb on + strand at 660.086 kb on - strand at 660.287 kb on - strand, within RR42_RS02970 at 660.671 kb on + strand, within RR42_RS02975 at 660.671 kb on + strand, within RR42_RS02975 at 660.675 kb on - strand, within RR42_RS02975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 657,165 + -0.5 657,568 - RR42_RS02960 0.45 -0.3 657,568 - RR42_RS02960 0.45 -0.8 657,670 + RR42_RS02960 0.56 -0.6 657,671 - RR42_RS02960 0.56 -0.9 658,053 - -0.5 658,100 + +0.9 658,319 + RR42_RS02965 0.15 +0.2 658,358 - RR42_RS02965 0.17 -0.9 658,370 + RR42_RS02965 0.18 -0.4 658,371 - RR42_RS02965 0.18 +0.0 658,371 - RR42_RS02965 0.18 +0.3 658,769 + RR42_RS02965 0.42 +0.9 658,769 + RR42_RS02965 0.42 -0.5 658,769 + RR42_RS02965 0.42 -0.4 658,770 - RR42_RS02965 0.42 -1.3 658,770 - RR42_RS02965 0.42 -0.2 658,869 - RR42_RS02965 0.48 -1.6 658,941 + RR42_RS02965 0.53 -1.3 658,985 + RR42_RS02965 0.55 -0.3 659,240 + RR42_RS02965 0.71 -0.3 659,306 + RR42_RS02965 0.75 +0.2 659,307 - RR42_RS02965 0.75 +0.6 659,330 + RR42_RS02965 0.76 -0.7 659,330 + RR42_RS02965 0.76 -1.6 659,330 + RR42_RS02965 0.76 +0.5 659,330 + RR42_RS02965 0.76 +0.8 659,331 - RR42_RS02965 0.76 -0.4 659,331 - RR42_RS02965 0.76 -0.2 659,815 - -1.0 659,825 + -1.3 659,826 - +1.2 659,880 - -0.4 659,919 + -0.5 659,948 + -0.1 659,949 - -0.8 660,005 + -0.5 660,006 - +0.4 660,006 - -0.1 660,026 - +0.1 660,085 + +0.6 660,085 + +1.2 660,086 - -0.1 660,287 - RR42_RS02970 0.51 -2.8 660,671 + RR42_RS02975 0.22 -2.5 660,671 + RR42_RS02975 0.22 +2.5 660,675 - RR42_RS02975 0.23 -0.1
Or see this region's nucleotide sequence