Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01590 and RR42_RS01595 are separated by 183 nucleotides RR42_RS01595 and RR42_RS01600 are separated by 367 nucleotides RR42_RS01600 and RR42_RS01605 are separated by 111 nucleotides RR42_RS01605 and RR42_RS01610 are separated by 72 nucleotides
RR42_RS01590: RR42_RS01590 - GntR family transcriptional regulator, at 359,928 to 361,334
_RS01590
RR42_RS01595: RR42_RS01595 - hypothetical protein, at 361,518 to 361,856
_RS01595
RR42_RS01600: RR42_RS01600 - methylmalonyl-CoA mutase, at 362,224 to 365,532
_RS01600
RR42_RS01605: RR42_RS01605 - hydrolase, at 365,644 to 366,078
_RS01605
RR42_RS01610: RR42_RS01610 - 3-hydroxybutyryl-CoA dehydrogenase, at 366,151 to 367,005
_RS01610
Position (kb) 362
363
364
365
366 Strain fitness (log2 ratio) -2
-1
0
1
2 at 361.414 kb on + strand at 361.415 kb on - strand at 361.430 kb on + strand at 361.430 kb on + strand at 361.431 kb on - strand at 361.431 kb on - strand at 361.431 kb on - strand at 361.528 kb on + strand at 361.529 kb on - strand at 361.529 kb on - strand at 361.912 kb on + strand at 361.912 kb on + strand at 361.918 kb on + strand at 361.918 kb on + strand at 361.919 kb on - strand at 361.919 kb on - strand at 361.919 kb on - strand at 362.022 kb on + strand at 362.023 kb on - strand at 362.023 kb on - strand at 362.039 kb on - strand at 362.039 kb on - strand at 362.039 kb on - strand at 362.095 kb on + strand at 362.428 kb on - strand at 362.527 kb on + strand at 362.527 kb on + strand at 362.629 kb on + strand, within RR42_RS01600 at 362.630 kb on - strand, within RR42_RS01600 at 362.732 kb on - strand, within RR42_RS01600 at 363.230 kb on - strand, within RR42_RS01600 at 363.818 kb on - strand, within RR42_RS01600 at 363.833 kb on - strand, within RR42_RS01600 at 363.988 kb on + strand, within RR42_RS01600 at 364.180 kb on + strand, within RR42_RS01600 at 364.181 kb on - strand, within RR42_RS01600 at 364.241 kb on - strand, within RR42_RS01600 at 364.535 kb on - strand, within RR42_RS01600 at 364.655 kb on - strand, within RR42_RS01600 at 364.667 kb on - strand, within RR42_RS01600 at 364.802 kb on - strand, within RR42_RS01600 at 364.834 kb on + strand, within RR42_RS01600 at 364.835 kb on - strand, within RR42_RS01600 at 365.209 kb on + strand at 365.213 kb on - strand at 365.213 kb on - strand at 365.259 kb on + strand at 365.259 kb on + strand at 365.260 kb on - strand at 365.505 kb on - strand at 365.526 kb on + strand at 365.527 kb on - strand at 365.572 kb on + strand at 365.653 kb on - strand at 365.674 kb on - strand at 365.674 kb on - strand at 365.717 kb on + strand, within RR42_RS01605 at 365.717 kb on + strand, within RR42_RS01605 at 365.718 kb on - strand, within RR42_RS01605 at 365.718 kb on - strand, within RR42_RS01605 at 365.720 kb on - strand, within RR42_RS01605 at 365.777 kb on + strand, within RR42_RS01605 at 365.778 kb on - strand, within RR42_RS01605 at 366.237 kb on - strand, within RR42_RS01610 at 366.237 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 361,414 + -0.4 361,415 - -0.6 361,430 + +0.2 361,430 + +0.5 361,431 - +0.9 361,431 - +1.7 361,431 - -0.1 361,528 + +0.5 361,529 - +0.4 361,529 - -0.2 361,912 + +0.8 361,912 + -0.3 361,918 + +0.3 361,918 + -0.7 361,919 - +0.2 361,919 - +0.6 361,919 - +0.5 362,022 + +0.3 362,023 - -0.1 362,023 - +0.6 362,039 - -0.3 362,039 - -0.3 362,039 - -0.0 362,095 + +0.7 362,428 - +0.2 362,527 + -1.1 362,527 + +0.1 362,629 + RR42_RS01600 0.12 -0.1 362,630 - RR42_RS01600 0.12 -0.4 362,732 - RR42_RS01600 0.15 +0.9 363,230 - RR42_RS01600 0.30 +0.0 363,818 - RR42_RS01600 0.48 +1.1 363,833 - RR42_RS01600 0.49 +1.7 363,988 + RR42_RS01600 0.53 +0.0 364,180 + RR42_RS01600 0.59 -0.1 364,181 - RR42_RS01600 0.59 -0.3 364,241 - RR42_RS01600 0.61 +0.6 364,535 - RR42_RS01600 0.70 +1.3 364,655 - RR42_RS01600 0.73 +0.8 364,667 - RR42_RS01600 0.74 +0.0 364,802 - RR42_RS01600 0.78 -0.0 364,834 + RR42_RS01600 0.79 -0.2 364,835 - RR42_RS01600 0.79 -0.6 365,209 + -0.4 365,213 - +0.0 365,213 - +1.5 365,259 + +1.1 365,259 + +0.4 365,260 - -1.5 365,505 - -0.2 365,526 + +0.8 365,527 - -0.0 365,572 + +0.4 365,653 - -1.0 365,674 - -0.1 365,674 - +0.0 365,717 + RR42_RS01605 0.17 -0.5 365,717 + RR42_RS01605 0.17 -0.3 365,718 - RR42_RS01605 0.17 -1.8 365,718 - RR42_RS01605 0.17 +0.6 365,720 - RR42_RS01605 0.17 -0.6 365,777 + RR42_RS01605 0.31 -0.2 365,778 - RR42_RS01605 0.31 -0.8 366,237 - RR42_RS01610 0.10 +1.3 366,237 - RR42_RS01610 0.10 +0.5 366,380 - RR42_RS01610 0.27 +0.2 366,380 - RR42_RS01610 0.27 -0.3 366,380 - RR42_RS01610 0.27 +1.9 366,380 - RR42_RS01610 0.27 +0.4
Or see this region's nucleotide sequence