Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1922 and Pf6N2E2_1923 are separated by 284 nucleotides Pf6N2E2_1923 and Pf6N2E2_1924 are separated by 418 nucleotides
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Pf6N2E2_1923 - Acetyl-CoA acetyltransferase, at 2,277,425 to 2,278,594
_1923
Pf6N2E2_1924 - 6-phosphogluconolactonase (EC 3.1.1.31), at 2,279,013 to 2,280,185
_1924
Position (kb)
2277
2278
2279 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.910 kb on - strand, within Pf6N2E2_1922 at 2276.942 kb on + strand at 2276.943 kb on - strand at 2277.420 kb on + strand at 2277.420 kb on + strand at 2277.432 kb on + strand at 2277.432 kb on + strand at 2277.433 kb on - strand at 2277.433 kb on - strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.461 kb on + strand at 2277.461 kb on + strand at 2277.507 kb on + strand at 2277.507 kb on + strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.798 kb on + strand, within Pf6N2E2_1923 at 2277.799 kb on - strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.842 kb on - strand, within Pf6N2E2_1923 at 2277.896 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.003 kb on - strand, within Pf6N2E2_1923 at 2278.079 kb on - strand, within Pf6N2E2_1923 at 2278.131 kb on + strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.268 kb on + strand, within Pf6N2E2_1923 at 2278.269 kb on - strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.417 kb on + strand, within Pf6N2E2_1923 at 2278.663 kb on - strand at 2278.846 kb on - strand at 2278.857 kb on + strand at 2278.857 kb on + strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.890 kb on - strand at 2279.034 kb on + strand at 2279.035 kb on - strand at 2279.045 kb on - strand at 2279.045 kb on - strand at 2279.064 kb on + strand at 2279.067 kb on - strand at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.267 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.303 kb on + strand, within Pf6N2E2_1924 at 2279.388 kb on - strand, within Pf6N2E2_1924 at 2279.436 kb on + strand, within Pf6N2E2_1924 at 2279.457 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,276,572 + Pf6N2E2_1922 0.73 +1.8 2,276,572 + Pf6N2E2_1922 0.73 -0.4 2,276,631 - Pf6N2E2_1922 0.76 -0.4 2,276,631 - Pf6N2E2_1922 0.76 +1.5 2,276,631 - Pf6N2E2_1922 0.76 -0.5 2,276,910 - Pf6N2E2_1922 0.89 +0.7 2,276,942 + +0.5 2,276,943 - -0.7 2,277,420 + +0.4 2,277,420 + -0.2 2,277,432 + +0.7 2,277,432 + -0.7 2,277,433 - -0.0 2,277,433 - -0.7 2,277,446 + +2.8 2,277,446 + +0.4 2,277,446 + -1.9 2,277,446 + +0.5 2,277,446 + -0.3 2,277,446 + -0.7 2,277,447 - +0.6 2,277,447 - +0.7 2,277,447 - -0.5 2,277,461 + -1.1 2,277,461 + +0.6 2,277,507 + -0.9 2,277,507 + -0.1 2,277,508 - +0.6 2,277,508 - -0.4 2,277,508 - -1.4 2,277,606 + Pf6N2E2_1923 0.15 +1.9 2,277,606 + Pf6N2E2_1923 0.15 -0.4 2,277,606 + Pf6N2E2_1923 0.15 +0.4 2,277,606 + Pf6N2E2_1923 0.15 +0.7 2,277,607 - Pf6N2E2_1923 0.16 -0.7 2,277,607 - Pf6N2E2_1923 0.16 +0.6 2,277,607 - Pf6N2E2_1923 0.16 -1.3 2,277,607 - Pf6N2E2_1923 0.16 +1.7 2,277,623 + Pf6N2E2_1923 0.17 -0.7 2,277,623 + Pf6N2E2_1923 0.17 +0.3 2,277,798 + Pf6N2E2_1923 0.32 +0.5 2,277,799 - Pf6N2E2_1923 0.32 -1.0 2,277,839 + Pf6N2E2_1923 0.35 +0.3 2,277,839 + Pf6N2E2_1923 0.35 +0.4 2,277,839 + Pf6N2E2_1923 0.35 -1.0 2,277,840 - Pf6N2E2_1923 0.35 +0.7 2,277,840 - Pf6N2E2_1923 0.35 -0.4 2,277,840 - Pf6N2E2_1923 0.35 +0.9 2,277,842 - Pf6N2E2_1923 0.36 +0.1 2,277,896 + Pf6N2E2_1923 0.40 +1.4 2,278,002 + Pf6N2E2_1923 0.49 +2.1 2,278,002 + Pf6N2E2_1923 0.49 +1.4 2,278,002 + Pf6N2E2_1923 0.49 -0.1 2,278,003 - Pf6N2E2_1923 0.49 +0.8 2,278,079 - Pf6N2E2_1923 0.56 -0.9 2,278,131 + Pf6N2E2_1923 0.60 +0.3 2,278,188 - Pf6N2E2_1923 0.65 -1.1 2,278,188 - Pf6N2E2_1923 0.65 +0.5 2,278,268 + Pf6N2E2_1923 0.72 -0.0 2,278,269 - Pf6N2E2_1923 0.72 +0.4 2,278,296 + Pf6N2E2_1923 0.74 -0.0 2,278,296 + Pf6N2E2_1923 0.74 -2.1 2,278,417 + Pf6N2E2_1923 0.85 +1.2 2,278,663 - +0.3 2,278,846 - -0.6 2,278,857 + +0.1 2,278,857 + +0.2 2,278,858 - -0.1 2,278,858 - +1.0 2,278,858 - -0.2 2,278,858 - -0.2 2,278,890 - +0.2 2,279,034 + +0.5 2,279,035 - +0.4 2,279,045 - -0.2 2,279,045 - +1.8 2,279,064 + +1.2 2,279,067 - +0.0 2,279,133 + Pf6N2E2_1924 0.10 -1.0 2,279,133 + Pf6N2E2_1924 0.10 -0.8 2,279,134 - Pf6N2E2_1924 0.10 -1.1 2,279,134 - Pf6N2E2_1924 0.10 +2.5 2,279,166 + Pf6N2E2_1924 0.13 -0.1 2,279,166 + Pf6N2E2_1924 0.13 -0.8 2,279,167 - Pf6N2E2_1924 0.13 +1.2 2,279,167 - Pf6N2E2_1924 0.13 +1.5 2,279,167 - Pf6N2E2_1924 0.13 +2.2 2,279,267 - Pf6N2E2_1924 0.22 -0.8 2,279,294 - Pf6N2E2_1924 0.24 -1.1 2,279,294 - Pf6N2E2_1924 0.24 +0.9 2,279,303 + Pf6N2E2_1924 0.25 -0.1 2,279,388 - Pf6N2E2_1924 0.32 +2.6 2,279,436 + Pf6N2E2_1924 0.36 -1.1 2,279,457 + Pf6N2E2_1924 0.38 +0.2 2,279,482 + Pf6N2E2_1924 0.40 +1.3 2,279,482 + Pf6N2E2_1924 0.40 -0.3
Or see this region's nucleotide sequence