Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1852 and Pf6N2E2_1853 are separated by 159 nucleotides Pf6N2E2_1853 and Pf6N2E2_1854 are separated by 116 nucleotides
Pf6N2E2_1852 - Regulatory protein, LuxR:Tetratricopeptide TPR_4, at 2,193,657 to 2,196,332
_1852
Pf6N2E2_1853 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,196,492 to 2,197,997
_1853
Pf6N2E2_1854 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,198,114 to 2,199,562
_1854
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2195.982 kb on - strand, within Pf6N2E2_1852 at 2196.007 kb on + strand, within Pf6N2E2_1852 at 2196.259 kb on + strand at 2196.259 kb on + strand at 2196.260 kb on - strand at 2196.260 kb on - strand at 2196.260 kb on - strand at 2196.373 kb on + strand at 2196.373 kb on + strand at 2196.374 kb on - strand at 2196.374 kb on - strand at 2196.387 kb on + strand at 2196.388 kb on - strand at 2196.762 kb on + strand, within Pf6N2E2_1853 at 2196.763 kb on - strand, within Pf6N2E2_1853 at 2196.784 kb on + strand, within Pf6N2E2_1853 at 2196.836 kb on - strand, within Pf6N2E2_1853 at 2196.836 kb on - strand, within Pf6N2E2_1853 at 2196.959 kb on - strand, within Pf6N2E2_1853 at 2197.011 kb on - strand, within Pf6N2E2_1853 at 2197.101 kb on - strand, within Pf6N2E2_1853 at 2197.101 kb on - strand, within Pf6N2E2_1853 at 2197.108 kb on + strand, within Pf6N2E2_1853 at 2197.109 kb on - strand, within Pf6N2E2_1853 at 2197.399 kb on + strand, within Pf6N2E2_1853 at 2197.400 kb on - strand, within Pf6N2E2_1853 at 2197.402 kb on + strand, within Pf6N2E2_1853 at 2197.402 kb on + strand, within Pf6N2E2_1853 at 2197.478 kb on + strand, within Pf6N2E2_1853 at 2197.478 kb on + strand, within Pf6N2E2_1853 at 2197.532 kb on + strand, within Pf6N2E2_1853 at 2197.533 kb on - strand, within Pf6N2E2_1853 at 2197.617 kb on + strand, within Pf6N2E2_1853 at 2197.618 kb on - strand, within Pf6N2E2_1853 at 2197.648 kb on + strand, within Pf6N2E2_1853 at 2197.648 kb on + strand, within Pf6N2E2_1853 at 2197.648 kb on + strand, within Pf6N2E2_1853 at 2197.722 kb on + strand, within Pf6N2E2_1853 at 2197.785 kb on - strand, within Pf6N2E2_1853 at 2197.792 kb on + strand, within Pf6N2E2_1853 at 2197.793 kb on - strand, within Pf6N2E2_1853 at 2197.796 kb on - strand, within Pf6N2E2_1853 at 2197.807 kb on + strand, within Pf6N2E2_1853 at 2197.824 kb on + strand, within Pf6N2E2_1853 at 2197.824 kb on + strand, within Pf6N2E2_1853 at 2197.824 kb on + strand, within Pf6N2E2_1853 at 2197.858 kb on + strand at 2197.859 kb on - strand at 2197.898 kb on - strand at 2197.898 kb on - strand at 2197.898 kb on - strand at 2197.921 kb on + strand at 2197.922 kb on - strand at 2197.925 kb on + strand at 2197.925 kb on + strand at 2197.939 kb on + strand at 2197.940 kb on - strand at 2197.992 kb on - strand at 2197.998 kb on - strand at 2197.998 kb on - strand at 2197.998 kb on - strand at 2197.998 kb on - strand at 2198.015 kb on - strand at 2198.094 kb on + strand at 2198.095 kb on - strand at 2198.130 kb on - strand at 2198.141 kb on + strand at 2198.142 kb on - strand at 2198.142 kb on - strand at 2198.179 kb on + strand at 2198.180 kb on - strand at 2198.180 kb on - strand at 2198.253 kb on + strand at 2198.254 kb on - strand at 2198.254 kb on - strand at 2198.395 kb on + strand, within Pf6N2E2_1854 at 2198.396 kb on - strand, within Pf6N2E2_1854 at 2198.397 kb on + strand, within Pf6N2E2_1854 at 2198.397 kb on + strand, within Pf6N2E2_1854 at 2198.397 kb on + strand, within Pf6N2E2_1854 at 2198.397 kb on + strand, within Pf6N2E2_1854 at 2198.398 kb on - strand, within Pf6N2E2_1854 at 2198.398 kb on - strand, within Pf6N2E2_1854 at 2198.398 kb on - strand, within Pf6N2E2_1854 at 2198.519 kb on + strand, within Pf6N2E2_1854 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.757 kb on + strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854 at 2198.758 kb on - strand, within Pf6N2E2_1854
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,195,982 - Pf6N2E2_1852 0.87 +0.9 2,196,007 + Pf6N2E2_1852 0.88 -1.2 2,196,259 + +0.2 2,196,259 + +0.9 2,196,260 - -0.6 2,196,260 - -1.2 2,196,260 - -0.3 2,196,373 + +0.4 2,196,373 + +2.8 2,196,374 - +0.5 2,196,374 - -0.3 2,196,387 + -0.1 2,196,388 - -0.5 2,196,762 + Pf6N2E2_1853 0.18 +1.1 2,196,763 - Pf6N2E2_1853 0.18 +0.3 2,196,784 + Pf6N2E2_1853 0.19 +1.8 2,196,836 - Pf6N2E2_1853 0.23 -1.1 2,196,836 - Pf6N2E2_1853 0.23 -1.9 2,196,959 - Pf6N2E2_1853 0.31 -0.1 2,197,011 - Pf6N2E2_1853 0.34 +0.6 2,197,101 - Pf6N2E2_1853 0.40 +0.3 2,197,101 - Pf6N2E2_1853 0.40 -0.5 2,197,108 + Pf6N2E2_1853 0.41 +0.7 2,197,109 - Pf6N2E2_1853 0.41 +1.0 2,197,399 + Pf6N2E2_1853 0.60 +0.4 2,197,400 - Pf6N2E2_1853 0.60 +1.1 2,197,402 + Pf6N2E2_1853 0.60 -0.1 2,197,402 + Pf6N2E2_1853 0.60 -1.7 2,197,478 + Pf6N2E2_1853 0.65 +1.7 2,197,478 + Pf6N2E2_1853 0.65 -1.0 2,197,532 + Pf6N2E2_1853 0.69 +0.7 2,197,533 - Pf6N2E2_1853 0.69 +0.3 2,197,617 + Pf6N2E2_1853 0.75 +1.7 2,197,618 - Pf6N2E2_1853 0.75 +0.7 2,197,648 + Pf6N2E2_1853 0.77 +2.2 2,197,648 + Pf6N2E2_1853 0.77 -1.3 2,197,648 + Pf6N2E2_1853 0.77 +0.4 2,197,722 + Pf6N2E2_1853 0.82 +1.2 2,197,785 - Pf6N2E2_1853 0.86 +0.8 2,197,792 + Pf6N2E2_1853 0.86 +0.7 2,197,793 - Pf6N2E2_1853 0.86 +0.9 2,197,796 - Pf6N2E2_1853 0.87 +0.5 2,197,807 + Pf6N2E2_1853 0.87 -0.0 2,197,824 + Pf6N2E2_1853 0.88 -0.5 2,197,824 + Pf6N2E2_1853 0.88 +1.4 2,197,824 + Pf6N2E2_1853 0.88 -1.4 2,197,858 + -0.0 2,197,859 - +1.2 2,197,898 - +1.4 2,197,898 - +0.6 2,197,898 - -0.6 2,197,921 + +0.2 2,197,922 - +0.1 2,197,925 + +0.6 2,197,925 + -0.2 2,197,939 + -1.6 2,197,940 - +0.9 2,197,992 - +1.4 2,197,998 - +0.7 2,197,998 - -1.7 2,197,998 - +0.3 2,197,998 - -0.0 2,198,015 - +0.6 2,198,094 + +0.8 2,198,095 - +0.6 2,198,130 - +0.2 2,198,141 + -1.9 2,198,142 - +1.0 2,198,142 - +0.1 2,198,179 + +0.6 2,198,180 - +0.6 2,198,180 - +0.6 2,198,253 + +1.2 2,198,254 - -0.9 2,198,254 - +1.0 2,198,395 + Pf6N2E2_1854 0.19 +1.1 2,198,396 - Pf6N2E2_1854 0.19 +0.2 2,198,397 + Pf6N2E2_1854 0.20 -0.0 2,198,397 + Pf6N2E2_1854 0.20 +0.9 2,198,397 + Pf6N2E2_1854 0.20 -0.8 2,198,397 + Pf6N2E2_1854 0.20 +0.9 2,198,398 - Pf6N2E2_1854 0.20 -2.0 2,198,398 - Pf6N2E2_1854 0.20 +0.2 2,198,398 - Pf6N2E2_1854 0.20 +0.9 2,198,519 + Pf6N2E2_1854 0.28 -0.0 2,198,757 + Pf6N2E2_1854 0.44 +1.7 2,198,757 + Pf6N2E2_1854 0.44 +1.9 2,198,757 + Pf6N2E2_1854 0.44 -0.6 2,198,758 - Pf6N2E2_1854 0.44 -0.0 2,198,758 - Pf6N2E2_1854 0.44 -0.1 2,198,758 - Pf6N2E2_1854 0.44 +1.0
Or see this region's nucleotide sequence