Experiment: ALP with Cisplatin 0.0007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3221 and Dsui_3222 are separated by 39 nucleotides Dsui_3222 and Dsui_3223 are separated by 140 nucleotides Dsui_3223 and Dsui_3224 are separated by 11 nucleotides Dsui_3224 and Dsui_3225 overlap by 4 nucleotides
Dsui_3221: Dsui_3221 - cytochrome c553, at 3,460,034 to 3,460,720
_3221
Dsui_3222: Dsui_3222 - oligoribonuclease (3'->5' exoribonuclease), at 3,460,760 to 3,461,305
_3222
Dsui_3223: Dsui_3223 - Zn-dependent protease with chaperone function, at 3,461,446 to 3,462,717
_3223
Dsui_3224: Dsui_3224 - pterin-4a-carbinolamine dehydratase, at 3,462,729 to 3,463,064
_3224
Dsui_3225: Dsui_3225 - ribosome small subunit-dependent GTPase A, at 3,463,061 to 3,463,948
_3225
Position (kb)
3461
3462
3463 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3460.498 kb on - strand, within Dsui_3221 at 3460.660 kb on + strand at 3460.673 kb on + strand at 3460.673 kb on + strand at 3460.680 kb on + strand at 3460.688 kb on - strand at 3460.722 kb on + strand at 3460.726 kb on + strand at 3461.345 kb on + strand at 3461.353 kb on - strand at 3461.356 kb on + strand at 3461.393 kb on + strand at 3461.394 kb on + strand at 3461.480 kb on + strand at 3461.484 kb on - strand at 3461.522 kb on - strand at 3461.537 kb on - strand at 3461.580 kb on + strand, within Dsui_3223 at 3461.617 kb on + strand, within Dsui_3223 at 3461.657 kb on + strand, within Dsui_3223 at 3461.672 kb on + strand, within Dsui_3223 at 3461.750 kb on + strand, within Dsui_3223 at 3461.758 kb on - strand, within Dsui_3223 at 3461.797 kb on - strand, within Dsui_3223 at 3461.858 kb on + strand, within Dsui_3223 at 3461.858 kb on + strand, within Dsui_3223 at 3461.863 kb on + strand, within Dsui_3223 at 3461.918 kb on + strand, within Dsui_3223 at 3461.962 kb on - strand at 3461.978 kb on + strand, within Dsui_3223 at 3461.986 kb on - strand at 3462.152 kb on + strand, within Dsui_3223 at 3462.219 kb on + strand, within Dsui_3223 at 3462.249 kb on + strand, within Dsui_3223 at 3462.280 kb on - strand, within Dsui_3223 at 3462.286 kb on - strand at 3462.287 kb on + strand, within Dsui_3223 at 3462.319 kb on + strand, within Dsui_3223 at 3462.327 kb on - strand, within Dsui_3223 at 3462.332 kb on - strand, within Dsui_3223 at 3462.366 kb on + strand, within Dsui_3223 at 3462.409 kb on + strand, within Dsui_3223 at 3462.427 kb on + strand, within Dsui_3223 at 3462.464 kb on + strand, within Dsui_3223 at 3462.517 kb on + strand, within Dsui_3223 at 3462.535 kb on + strand, within Dsui_3223 at 3462.556 kb on - strand, within Dsui_3223 at 3462.563 kb on - strand, within Dsui_3223 at 3462.570 kb on + strand, within Dsui_3223 at 3462.605 kb on + strand at 3462.605 kb on + strand at 3462.605 kb on + strand at 3462.687 kb on + strand at 3462.698 kb on + strand at 3462.720 kb on - strand at 3462.740 kb on - strand at 3462.768 kb on - strand, within Dsui_3224 at 3462.962 kb on + strand, within Dsui_3224 at 3462.965 kb on - strand, within Dsui_3224 at 3462.970 kb on - strand, within Dsui_3224 at 3463.072 kb on + strand at 3463.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0007 mg/ml remove 3,460,498 - Dsui_3221 0.68 -0.4 3,460,660 + +0.6 3,460,673 + -1.4 3,460,673 + -1.0 3,460,680 + -1.7 3,460,688 - -0.3 3,460,722 + +0.6 3,460,726 + +0.6 3,461,345 + +0.2 3,461,353 - +0.6 3,461,356 + +0.6 3,461,393 + +0.0 3,461,394 + -2.7 3,461,480 + -0.6 3,461,484 - -1.4 3,461,522 - +0.6 3,461,537 - -0.4 3,461,580 + Dsui_3223 0.11 -0.3 3,461,617 + Dsui_3223 0.13 -0.2 3,461,657 + Dsui_3223 0.17 -1.4 3,461,672 + Dsui_3223 0.18 -0.2 3,461,750 + Dsui_3223 0.24 -0.2 3,461,758 - Dsui_3223 0.25 +0.6 3,461,797 - Dsui_3223 0.28 -1.4 3,461,858 + Dsui_3223 0.32 -0.5 3,461,858 + Dsui_3223 0.32 -0.2 3,461,863 + Dsui_3223 0.33 -0.3 3,461,918 + Dsui_3223 0.37 -1.7 3,461,962 - +0.6 3,461,978 + Dsui_3223 0.42 -1.5 3,461,986 - +0.6 3,462,152 + Dsui_3223 0.56 -0.4 3,462,219 + Dsui_3223 0.61 -0.1 3,462,249 + Dsui_3223 0.63 -0.8 3,462,280 - Dsui_3223 0.66 +0.6 3,462,286 - +0.6 3,462,287 + Dsui_3223 0.66 +0.1 3,462,319 + Dsui_3223 0.69 -0.5 3,462,327 - Dsui_3223 0.69 -1.7 3,462,332 - Dsui_3223 0.70 -0.4 3,462,366 + Dsui_3223 0.72 -0.4 3,462,409 + Dsui_3223 0.76 -0.9 3,462,427 + Dsui_3223 0.77 -0.9 3,462,464 + Dsui_3223 0.80 -0.4 3,462,517 + Dsui_3223 0.84 -0.4 3,462,535 + Dsui_3223 0.86 -2.9 3,462,556 - Dsui_3223 0.87 -0.4 3,462,563 - Dsui_3223 0.88 -1.4 3,462,570 + Dsui_3223 0.88 -1.1 3,462,605 + -0.7 3,462,605 + -0.7 3,462,605 + -0.1 3,462,687 + -0.2 3,462,698 + -1.1 3,462,720 - +0.6 3,462,740 - -0.4 3,462,768 - Dsui_3224 0.12 +0.6 3,462,962 + Dsui_3224 0.69 -1.4 3,462,965 - Dsui_3224 0.70 +0.6 3,462,970 - Dsui_3224 0.72 -1.0 3,463,072 + -0.8 3,463,079 + -3.7
Or see this region's nucleotide sequence