Experiment: ALP with Cisplatin 0.0007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3126 and Dsui_3127 are separated by 201 nucleotides Dsui_3127 and Dsui_3128 are separated by 16 nucleotides Dsui_3128 and Dsui_3129 are separated by 40 nucleotides Dsui_3129 and Dsui_3130 are separated by 244 nucleotides Dsui_3130 and Dsui_3131 are separated by 269 nucleotides
Dsui_3126: Dsui_3126 - heme d1 biosynthesis radical SAM protein NirJ, at 3,348,006 to 3,349,169
_3126
Dsui_3127: Dsui_3127 - 23S RNA-specific pseudouridylate synthase, at 3,349,371 to 3,350,117
_3127
Dsui_3128: Dsui_3128 - ribonuclease R, at 3,350,134 to 3,352,620
_3128
Dsui_3129: Dsui_3129 - tRNA-Leu, at 3,352,661 to 3,352,747
_3129
Dsui_3130: Dsui_3130 - tRNA-Leu, at 3,352,992 to 3,353,078
_3130
Dsui_3131: Dsui_3131 - adenylosuccinate synthase, at 3,353,348 to 3,354,658
_3131
Position (kb)
3350
3351
3352
3353 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3349.177 kb on + strand at 3349.234 kb on + strand at 3349.234 kb on + strand at 3349.245 kb on - strand at 3349.252 kb on - strand at 3349.336 kb on - strand at 3349.341 kb on + strand at 3349.616 kb on + strand, within Dsui_3127 at 3349.621 kb on + strand, within Dsui_3127 at 3349.778 kb on - strand at 3350.016 kb on - strand, within Dsui_3127 at 3350.065 kb on - strand at 3350.080 kb on - strand at 3350.468 kb on + strand, within Dsui_3128 at 3350.562 kb on + strand, within Dsui_3128 at 3350.589 kb on + strand, within Dsui_3128 at 3350.626 kb on + strand, within Dsui_3128 at 3350.776 kb on - strand, within Dsui_3128 at 3350.853 kb on + strand, within Dsui_3128 at 3350.853 kb on + strand at 3350.861 kb on - strand, within Dsui_3128 at 3350.895 kb on - strand at 3350.896 kb on - strand, within Dsui_3128 at 3350.954 kb on - strand, within Dsui_3128 at 3350.961 kb on + strand, within Dsui_3128 at 3350.969 kb on - strand, within Dsui_3128 at 3350.996 kb on + strand at 3351.003 kb on - strand, within Dsui_3128 at 3351.004 kb on - strand, within Dsui_3128 at 3351.028 kb on - strand at 3351.032 kb on + strand, within Dsui_3128 at 3351.144 kb on + strand at 3351.207 kb on + strand at 3351.256 kb on - strand, within Dsui_3128 at 3351.352 kb on + strand, within Dsui_3128 at 3351.360 kb on + strand, within Dsui_3128 at 3351.360 kb on - strand, within Dsui_3128 at 3351.372 kb on - strand, within Dsui_3128 at 3351.444 kb on + strand, within Dsui_3128 at 3351.452 kb on - strand, within Dsui_3128 at 3351.468 kb on + strand, within Dsui_3128 at 3351.499 kb on - strand, within Dsui_3128 at 3351.590 kb on + strand, within Dsui_3128 at 3351.622 kb on + strand, within Dsui_3128 at 3351.636 kb on - strand, within Dsui_3128 at 3351.643 kb on + strand, within Dsui_3128 at 3351.643 kb on + strand at 3351.643 kb on + strand, within Dsui_3128 at 3351.651 kb on - strand, within Dsui_3128 at 3351.657 kb on - strand, within Dsui_3128 at 3351.753 kb on - strand, within Dsui_3128 at 3351.778 kb on + strand, within Dsui_3128 at 3351.817 kb on + strand, within Dsui_3128 at 3351.817 kb on + strand at 3351.834 kb on + strand, within Dsui_3128 at 3351.963 kb on + strand, within Dsui_3128 at 3352.022 kb on + strand, within Dsui_3128 at 3352.030 kb on - strand, within Dsui_3128 at 3352.070 kb on - strand, within Dsui_3128 at 3352.130 kb on - strand, within Dsui_3128 at 3352.151 kb on - strand, within Dsui_3128 at 3352.201 kb on + strand at 3352.257 kb on + strand, within Dsui_3128 at 3352.324 kb on + strand, within Dsui_3128 at 3352.325 kb on + strand, within Dsui_3128 at 3352.331 kb on + strand, within Dsui_3128 at 3352.332 kb on - strand, within Dsui_3128 at 3352.332 kb on - strand, within Dsui_3128 at 3352.415 kb on - strand at 3352.438 kb on + strand at 3352.958 kb on - strand at 3353.061 kb on + strand, within Dsui_3130 at 3353.232 kb on + strand at 3353.269 kb on + strand at 3353.269 kb on + strand at 3353.277 kb on - strand at 3353.277 kb on - strand at 3353.277 kb on - strand at 3353.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0007 mg/ml remove 3,349,177 + +0.6 3,349,234 + -1.7 3,349,234 + -0.4 3,349,245 - +0.6 3,349,252 - -0.4 3,349,336 - -1.7 3,349,341 + -0.7 3,349,616 + Dsui_3127 0.33 -0.6 3,349,621 + Dsui_3127 0.33 -2.2 3,349,778 - -0.4 3,350,016 - Dsui_3127 0.86 -1.0 3,350,065 - -2.0 3,350,080 - +0.6 3,350,468 + Dsui_3128 0.13 -0.4 3,350,562 + Dsui_3128 0.17 -1.4 3,350,589 + Dsui_3128 0.18 +0.6 3,350,626 + Dsui_3128 0.20 -1.0 3,350,776 - Dsui_3128 0.26 -2.9 3,350,853 + Dsui_3128 0.29 -0.4 3,350,853 + +0.6 3,350,861 - Dsui_3128 0.29 -1.2 3,350,895 - -1.0 3,350,896 - Dsui_3128 0.31 -0.3 3,350,954 - Dsui_3128 0.33 -1.6 3,350,961 + Dsui_3128 0.33 -1.4 3,350,969 - Dsui_3128 0.34 +1.0 3,350,996 + +0.6 3,351,003 - Dsui_3128 0.35 -0.4 3,351,004 - Dsui_3128 0.35 -1.2 3,351,028 - -0.4 3,351,032 + Dsui_3128 0.36 +0.6 3,351,144 + +0.6 3,351,207 + +0.6 3,351,256 - Dsui_3128 0.45 +1.6 3,351,352 + Dsui_3128 0.49 +0.6 3,351,360 + Dsui_3128 0.49 -0.4 3,351,360 - Dsui_3128 0.49 -0.6 3,351,372 - Dsui_3128 0.50 -1.3 3,351,444 + Dsui_3128 0.53 -1.4 3,351,452 - Dsui_3128 0.53 -1.8 3,351,468 + Dsui_3128 0.54 -1.0 3,351,499 - Dsui_3128 0.55 -0.4 3,351,590 + Dsui_3128 0.59 -0.4 3,351,622 + Dsui_3128 0.60 +0.6 3,351,636 - Dsui_3128 0.60 -2.1 3,351,643 + Dsui_3128 0.61 -0.4 3,351,643 + +0.6 3,351,643 + Dsui_3128 0.61 -0.4 3,351,651 - Dsui_3128 0.61 -1.6 3,351,657 - Dsui_3128 0.61 -0.2 3,351,753 - Dsui_3128 0.65 -0.5 3,351,778 + Dsui_3128 0.66 -1.7 3,351,817 + Dsui_3128 0.68 -1.4 3,351,817 + -1.4 3,351,834 + Dsui_3128 0.68 -1.0 3,351,963 + Dsui_3128 0.74 -0.4 3,352,022 + Dsui_3128 0.76 -1.0 3,352,030 - Dsui_3128 0.76 -0.4 3,352,070 - Dsui_3128 0.78 +2.4 3,352,130 - Dsui_3128 0.80 -3.2 3,352,151 - Dsui_3128 0.81 -1.0 3,352,201 + +0.6 3,352,257 + Dsui_3128 0.85 +0.6 3,352,324 + Dsui_3128 0.88 -0.4 3,352,325 + Dsui_3128 0.88 -2.0 3,352,331 + Dsui_3128 0.88 -1.7 3,352,332 - Dsui_3128 0.88 +1.1 3,352,332 - Dsui_3128 0.88 -2.1 3,352,415 - -0.1 3,352,438 + -1.4 3,352,958 - +0.4 3,353,061 + Dsui_3130 0.79 -0.6 3,353,232 + -0.6 3,353,269 + -0.6 3,353,269 + -1.6 3,353,277 - -0.2 3,353,277 - -0.6 3,353,277 - -0.5 3,353,339 - -0.3
Or see this region's nucleotide sequence