Experiment: ALP with Cisplatin 0.0007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1097 and Dsui_1098 are separated by 108 nucleotides Dsui_1098 and Dsui_1099 are separated by 57 nucleotides Dsui_1099 and Dsui_1100 are separated by 9 nucleotides Dsui_1100 and Dsui_1101 are separated by 3 nucleotides
Dsui_1097: Dsui_1097 - NADH/NADPH-dependent glutamate synthase small subunit, at 1,139,961 to 1,141,427
_1097
Dsui_1098: Dsui_1098 - hypothetical protein, at 1,141,536 to 1,142,249
_1098
Dsui_1099: Dsui_1099 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 1,142,307 to 1,143,665
_1099
Dsui_1100: Dsui_1100 - hypothetical protein, at 1,143,675 to 1,143,983
_1100
Dsui_1101: Dsui_1101 - glucosamine--fructose-6-phosphate aminotransferase, isomerizing, at 1,143,987 to 1,145,813
_1101
Position (kb)
1142
1143
1144 Strain fitness (log2 ratio)
-1
0
1 at 1141.677 kb on + strand, within Dsui_1098 at 1141.765 kb on + strand, within Dsui_1098 at 1141.827 kb on + strand, within Dsui_1098 at 1141.901 kb on + strand, within Dsui_1098 at 1142.116 kb on - strand, within Dsui_1098 at 1142.171 kb on + strand, within Dsui_1098 at 1142.256 kb on + strand at 1142.256 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0007 mg/ml remove 1,141,677 + Dsui_1098 0.20 +0.2 1,141,765 + Dsui_1098 0.32 +0.2 1,141,827 + Dsui_1098 0.41 +0.0 1,141,901 + Dsui_1098 0.51 -0.5 1,142,116 - Dsui_1098 0.81 -1.0 1,142,171 + Dsui_1098 0.89 -0.5 1,142,256 + -0.2 1,142,256 + -0.2
Or see this region's nucleotide sequence