Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05185

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05180 and BWI76_RS05185 are separated by 191 nucleotidesBWI76_RS05185 and BWI76_RS05190 are separated by 46 nucleotidesBWI76_RS05190 and BWI76_RS05195 are separated by 107 nucleotides BWI76_RS05180: BWI76_RS05180 - serine endoprotease DegQ, at 1,089,597 to 1,091,036 _RS05180 BWI76_RS05185: BWI76_RS05185 - CdaR family transcriptional regulator, at 1,091,228 to 1,092,385 _RS05185 BWI76_RS05190: BWI76_RS05190 - hypothetical protein, at 1,092,432 to 1,092,821 _RS05190 BWI76_RS05195: BWI76_RS05195 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,092,929 to 1,093,753 _RS05195 Position (kb) 1091 1092 1093Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1090.247 kb on - strand, within BWI76_RS05180at 1090.247 kb on - strand, within BWI76_RS05180at 1090.331 kb on + strand, within BWI76_RS05180at 1090.338 kb on - strand, within BWI76_RS05180at 1090.606 kb on - strand, within BWI76_RS05180at 1090.800 kb on + strand, within BWI76_RS05180at 1090.806 kb on - strand, within BWI76_RS05180at 1090.942 kb on + strandat 1091.147 kb on - strandat 1091.200 kb on + strandat 1091.263 kb on + strandat 1091.271 kb on - strandat 1091.457 kb on + strand, within BWI76_RS05185at 1091.548 kb on + strand, within BWI76_RS05185at 1091.616 kb on - strand, within BWI76_RS05185at 1091.627 kb on + strand, within BWI76_RS05185at 1091.662 kb on - strand, within BWI76_RS05185at 1091.803 kb on + strand, within BWI76_RS05185at 1091.806 kb on - strand, within BWI76_RS05185at 1091.806 kb on - strand, within BWI76_RS05185at 1092.204 kb on + strand, within BWI76_RS05185at 1092.268 kb on + strand, within BWI76_RS05185at 1092.308 kb on - strandat 1092.344 kb on + strandat 1092.354 kb on + strandat 1092.357 kb on - strandat 1092.357 kb on - strandat 1092.374 kb on - strandat 1092.599 kb on - strand, within BWI76_RS05190at 1092.599 kb on - strand, within BWI76_RS05190at 1092.604 kb on - strand, within BWI76_RS05190at 1092.627 kb on + strand, within BWI76_RS05190at 1092.627 kb on + strand, within BWI76_RS05190at 1092.627 kb on + strand, within BWI76_RS05190at 1092.627 kb on - strandat 1092.635 kb on - strand, within BWI76_RS05190at 1092.635 kb on - strand, within BWI76_RS05190at 1092.635 kb on - strand, within BWI76_RS05190at 1092.706 kb on + strand, within BWI76_RS05190at 1092.706 kb on + strand, within BWI76_RS05190at 1092.714 kb on - strand, within BWI76_RS05190at 1092.755 kb on + strand, within BWI76_RS05190at 1092.784 kb on + strandat 1092.789 kb on + strandat 1092.794 kb on + strandat 1092.802 kb on - strandat 1092.818 kb on + strandat 1092.837 kb on - strandat 1092.927 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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1,090,247 - BWI76_RS05180 0.45 -2.9
1,090,247 - BWI76_RS05180 0.45 -0.4
1,090,331 + BWI76_RS05180 0.51 -2.7
1,090,338 - BWI76_RS05180 0.51 -2.1
1,090,606 - BWI76_RS05180 0.70 -2.3
1,090,800 + BWI76_RS05180 0.84 -1.9
1,090,806 - BWI76_RS05180 0.84 -2.9
1,090,942 + -0.1
1,091,147 - +0.8
1,091,200 + +0.4
1,091,263 + +0.3
1,091,271 - +0.4
1,091,457 + BWI76_RS05185 0.20 +1.6
1,091,548 + BWI76_RS05185 0.28 -0.6
1,091,616 - BWI76_RS05185 0.34 +0.7
1,091,627 + BWI76_RS05185 0.34 -0.3
1,091,662 - BWI76_RS05185 0.37 +0.7
1,091,803 + BWI76_RS05185 0.50 -0.2
1,091,806 - BWI76_RS05185 0.50 -0.4
1,091,806 - BWI76_RS05185 0.50 -0.5
1,092,204 + BWI76_RS05185 0.84 +0.4
1,092,268 + BWI76_RS05185 0.90 +0.0
1,092,308 - +0.0
1,092,344 + -0.2
1,092,354 + -0.1
1,092,357 - +0.9
1,092,357 - -0.9
1,092,374 - +0.4
1,092,599 - BWI76_RS05190 0.43 -0.1
1,092,599 - BWI76_RS05190 0.43 -1.2
1,092,604 - BWI76_RS05190 0.44 -0.9
1,092,627 + BWI76_RS05190 0.50 -0.8
1,092,627 + BWI76_RS05190 0.50 +0.2
1,092,627 + BWI76_RS05190 0.50 +0.0
1,092,627 - +4.0
1,092,635 - BWI76_RS05190 0.52 +0.6
1,092,635 - BWI76_RS05190 0.52 -0.1
1,092,635 - BWI76_RS05190 0.52 +0.8
1,092,706 + BWI76_RS05190 0.70 +0.0
1,092,706 + BWI76_RS05190 0.70 -0.2
1,092,714 - BWI76_RS05190 0.72 -0.2
1,092,755 + BWI76_RS05190 0.83 +0.4
1,092,784 + -0.4
1,092,789 + +0.3
1,092,794 + +0.1
1,092,802 - -2.4
1,092,818 + -0.1
1,092,837 - +0.3
1,092,927 + +1.1

Or see this region's nucleotide sequence