Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0672 and BT0673 are separated by 90 nucleotides BT0673 and BT0674 are separated by 176 nucleotides BT0674 and tRNA-Gly are separated by 118 nucleotides tRNA-Gly and tRNA-Leu are separated by 34 nucleotides tRNA-Leu and tRNA-Leu are separated by 21 nucleotides tRNA-Leu and tRNA-Gly are separated by 18 nucleotides tRNA-Gly and tRNA-Leu are separated by 32 nucleotides tRNA-Leu and tRNA-Gly are separated by 35 nucleotides tRNA-Gly and BT0675 are separated by 125 nucleotides
BT0672: BT0672 - drug efflux protein (NCBI ptt file), at 830,248 to 833,493
BT0672
BT0673: BT0673 - NADP(H) oxidoreductase (NCBI ptt file), at 833,584 to 834,138
BT0673
BT0674: BT0674 - carboxynorspermidine decarboxylase (NCBI ptt file), at 834,315 to 835,454
BT0674
BT_t03: tRNA-Gly - tRNA-Gly (RefSeq), at 835,573 to 835,645
tRNA-Gly
BT_t04: tRNA-Leu - tRNA-Leu (RefSeq), at 835,680 to 835,764
tRNA-Leu
BT_t05: tRNA-Leu - tRNA-Leu (RefSeq), at 835,786 to 835,869
tRNA-Leu
BT_t06: tRNA-Gly - tRNA-Gly (RefSeq), at 835,888 to 835,960
tRNA-Gly
BT_t07: tRNA-Leu - tRNA-Leu (RefSeq), at 835,993 to 836,077
tRNA-Leu
BT_t08: tRNA-Gly - tRNA-Gly (RefSeq), at 836,113 to 836,188
tRNA-Gly
BT0675: BT0675 - N-acetylglucosamine-6-phosphate deacetylase (NCBI ptt file), at 836,314 to 837,480
BT0675
Position (kb) 834
835
836 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 833.397 kb on + strand at 833.398 kb on - strand at 833.415 kb on - strand at 833.509 kb on - strand at 833.516 kb on + strand at 833.517 kb on - strand at 833.553 kb on + strand at 833.557 kb on + strand at 833.571 kb on + strand at 833.573 kb on + strand at 833.580 kb on + strand at 833.581 kb on - strand at 833.640 kb on - strand, within BT0673 at 833.798 kb on + strand, within BT0673 at 833.799 kb on - strand, within BT0673 at 833.828 kb on - strand, within BT0673 at 833.943 kb on - strand, within BT0673 at 834.154 kb on + strand at 834.348 kb on + strand at 834.353 kb on + strand at 834.354 kb on - strand at 834.354 kb on - strand at 834.511 kb on - strand, within BT0674 at 834.623 kb on + strand, within BT0674 at 834.737 kb on + strand, within BT0674 at 834.888 kb on + strand, within BT0674 at 835.047 kb on - strand, within BT0674 at 835.409 kb on - strand at 835.877 kb on + strand at 836.250 kb on + strand at 836.251 kb on - strand at 836.251 kb on - strand at 836.291 kb on + strand at 836.301 kb on - strand at 836.313 kb on + strand at 836.331 kb on + strand at 836.378 kb on + strand at 836.378 kb on + strand at 836.379 kb on - strand at 836.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 833,397 + +1.6 833,398 - +1.1 833,415 - -0.6 833,509 - -0.4 833,516 + -0.1 833,517 - -1.9 833,553 + -0.5 833,557 + -0.4 833,571 + -1.7 833,573 + -0.9 833,580 + -0.9 833,581 - -0.7 833,640 - BT0673 0.10 -1.1 833,798 + BT0673 0.39 -1.0 833,799 - BT0673 0.39 +0.1 833,828 - BT0673 0.44 +3.2 833,943 - BT0673 0.65 -2.9 834,154 + +1.4 834,348 + -2.2 834,353 + -2.2 834,354 - -1.5 834,354 - -2.3 834,511 - BT0674 0.17 -1.5 834,623 + BT0674 0.27 -2.7 834,737 + BT0674 0.37 -4.1 834,888 + BT0674 0.50 -0.4 835,047 - BT0674 0.64 -2.1 835,409 - -1.8 835,877 + +0.1 836,250 + -1.2 836,251 - -0.3 836,251 - -1.2 836,291 + -1.2 836,301 - +0.3 836,313 + -0.7 836,331 + -0.5 836,378 + +0.6 836,378 + +0.1 836,379 - -2.4 836,383 - -0.7
Or see this region's nucleotide sequence