Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5654 and Pf1N1B4_5655 are separated by 927 nucleotides Pf1N1B4_5655 and Pf1N1B4_5656 are separated by 11 nucleotides Pf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotides
Pf1N1B4_5654 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 85,468 to 86,529
_5654
Pf1N1B4_5655 - FIG00955561: hypothetical protein, at 87,457 to 87,663
_5655
Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382
_5656
Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792
_5657
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 86.802 kb on + strand at 86.802 kb on + strand at 86.802 kb on + strand at 86.803 kb on - strand at 86.803 kb on - strand at 86.844 kb on - strand at 87.110 kb on + strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.178 kb on - strand at 87.249 kb on + strand at 87.250 kb on - strand at 87.581 kb on - strand at 87.787 kb on - strand, within Pf1N1B4_5656 at 87.986 kb on + strand at 87.986 kb on + strand, within Pf1N1B4_5656 at 88.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 86,802 + -1.6 86,802 + +0.7 86,802 + +0.3 86,803 - -0.7 86,803 - -1.9 86,844 - -0.1 87,110 + +0.1 87,111 - -1.0 87,111 - +0.1 87,111 - -3.3 87,178 - +1.2 87,249 + -0.7 87,250 - -0.3 87,581 - -0.3 87,787 - Pf1N1B4_5656 0.16 -2.6 87,986 + +0.0 87,986 + Pf1N1B4_5656 0.44 -0.3 88,372 - -0.1
Or see this region's nucleotide sequence