Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5082 and Pf1N1B4_5083 are separated by 24 nucleotides Pf1N1B4_5083 and Pf1N1B4_5084 are separated by 2 nucleotides Pf1N1B4_5084 and Pf1N1B4_5085 are separated by 139 nucleotides
Pf1N1B4_5082 - Capsular polysaccharide synthesis enzyme CpsB, at 5,631,074 to 5,632,228
_5082
Pf1N1B4_5083 - Capsular polysaccharide synthesis enzyme CpsC, polysaccharide export, at 5,632,253 to 5,632,774
_5083
Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023
_5084
Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554
_5085
Position (kb) 5632
5633
5634
5635
5636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5631.812 kb on + strand, within Pf1N1B4_5082 at 5631.900 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.219 kb on - strand at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.554 kb on - strand, within Pf1N1B4_5083 at 5632.773 kb on - strand at 5632.786 kb on - strand at 5632.799 kb on - strand at 5633.072 kb on - strand at 5633.363 kb on + strand, within Pf1N1B4_5084 at 5633.643 kb on + strand at 5633.679 kb on - strand, within Pf1N1B4_5084 at 5633.925 kb on - strand, within Pf1N1B4_5084 at 5633.981 kb on + strand, within Pf1N1B4_5084 at 5633.982 kb on - strand, within Pf1N1B4_5084 at 5634.447 kb on - strand, within Pf1N1B4_5084 at 5634.844 kb on + strand at 5634.845 kb on - strand at 5634.882 kb on - strand at 5635.010 kb on - strand at 5635.022 kb on - strand at 5635.065 kb on - strand at 5635.065 kb on - strand at 5635.092 kb on + strand at 5635.092 kb on + strand at 5635.346 kb on + strand, within Pf1N1B4_5085 at 5635.551 kb on - strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.628 kb on - strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.830 kb on - strand, within Pf1N1B4_5085 at 5635.937 kb on + strand, within Pf1N1B4_5085 at 5636.023 kb on + strand, within Pf1N1B4_5085
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 5,631,812 + Pf1N1B4_5082 0.64 +1.7 5,631,900 - Pf1N1B4_5082 0.72 +0.6 5,632,089 - +1.4 5,632,089 - Pf1N1B4_5082 0.88 -0.1 5,632,089 - Pf1N1B4_5082 0.88 -2.4 5,632,219 - +2.7 5,632,342 - Pf1N1B4_5083 0.17 -0.4 5,632,342 - Pf1N1B4_5083 0.17 -3.0 5,632,554 - Pf1N1B4_5083 0.58 +0.6 5,632,773 - +2.1 5,632,786 - -0.4 5,632,799 - +0.9 5,633,072 - +0.2 5,633,363 + Pf1N1B4_5084 0.26 -2.2 5,633,643 + -1.4 5,633,679 - Pf1N1B4_5084 0.40 -0.0 5,633,925 - Pf1N1B4_5084 0.51 -0.6 5,633,981 + Pf1N1B4_5084 0.54 -1.0 5,633,982 - Pf1N1B4_5084 0.54 -0.2 5,634,447 - Pf1N1B4_5084 0.74 -0.4 5,634,844 + +0.2 5,634,845 - +0.6 5,634,882 - +1.2 5,635,010 - +0.6 5,635,022 - +1.2 5,635,065 - -2.4 5,635,065 - +0.3 5,635,092 + -1.4 5,635,092 + -0.1 5,635,346 + Pf1N1B4_5085 0.13 +0.2 5,635,551 - Pf1N1B4_5085 0.28 -0.0 5,635,627 + Pf1N1B4_5085 0.33 +0.9 5,635,627 + Pf1N1B4_5085 0.33 -2.0 5,635,628 - Pf1N1B4_5085 0.33 -0.0 5,635,773 + Pf1N1B4_5085 0.44 +0.6 5,635,773 + Pf1N1B4_5085 0.44 +1.2 5,635,785 - Pf1N1B4_5085 0.45 +1.1 5,635,785 - Pf1N1B4_5085 0.45 +0.4 5,635,830 - Pf1N1B4_5085 0.48 +1.0 5,635,937 + Pf1N1B4_5085 0.56 +0.2 5,636,023 + Pf1N1B4_5085 0.62 +0.2
Or see this region's nucleotide sequence