Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5039 and Pf1N1B4_5040 are separated by 86 nucleotides Pf1N1B4_5040 and Pf1N1B4_5041 are separated by 31 nucleotides Pf1N1B4_5041 and Pf1N1B4_5042 overlap by 1 nucleotides Pf1N1B4_5042 and Pf1N1B4_5043 are separated by 289 nucleotides Pf1N1B4_5043 and Pf1N1B4_5044 are separated by 402 nucleotides
Pf1N1B4_5039 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,584,394 to 5,585,167
_5039
Pf1N1B4_5040 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 5,585,254 to 5,585,694
_5040
Pf1N1B4_5041 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 5,585,726 to 5,586,112
_5041
Pf1N1B4_5042 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 5,586,112 to 5,588,811
_5042
Pf1N1B4_5043 - hypothetical protein, at 5,589,101 to 5,589,232
_5043
Pf1N1B4_5044 - hypothetical protein, at 5,589,635 to 5,589,994
_5044
Position (kb)
5586
5587
5588
5589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.136 kb on - strand at 5585.136 kb on - strand at 5585.270 kb on + strand at 5585.270 kb on + strand at 5585.271 kb on - strand at 5585.271 kb on - strand at 5585.422 kb on - strand, within Pf1N1B4_5040 at 5585.519 kb on + strand, within Pf1N1B4_5040 at 5585.520 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.838 kb on + strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.937 kb on + strand, within Pf1N1B4_5041 at 5585.938 kb on - strand, within Pf1N1B4_5041 at 5586.437 kb on + strand at 5586.437 kb on + strand, within Pf1N1B4_5042 at 5586.438 kb on - strand, within Pf1N1B4_5042 at 5586.542 kb on + strand, within Pf1N1B4_5042 at 5586.876 kb on - strand, within Pf1N1B4_5042 at 5586.962 kb on + strand, within Pf1N1B4_5042 at 5586.962 kb on + strand, within Pf1N1B4_5042 at 5586.962 kb on + strand, within Pf1N1B4_5042 at 5586.962 kb on + strand, within Pf1N1B4_5042 at 5587.169 kb on + strand, within Pf1N1B4_5042 at 5587.187 kb on + strand, within Pf1N1B4_5042 at 5587.497 kb on - strand, within Pf1N1B4_5042 at 5587.517 kb on + strand, within Pf1N1B4_5042 at 5587.517 kb on + strand at 5587.517 kb on + strand at 5587.517 kb on + strand, within Pf1N1B4_5042 at 5587.517 kb on + strand, within Pf1N1B4_5042 at 5587.518 kb on - strand, within Pf1N1B4_5042 at 5587.826 kb on + strand, within Pf1N1B4_5042 at 5587.827 kb on - strand, within Pf1N1B4_5042 at 5588.427 kb on + strand, within Pf1N1B4_5042 at 5588.676 kb on + strand at 5588.676 kb on + strand at 5588.704 kb on + strand at 5589.046 kb on + strand at 5589.096 kb on + strand at 5589.096 kb on + strand at 5589.097 kb on - strand at 5589.119 kb on + strand, within Pf1N1B4_5043 at 5589.119 kb on + strand, within Pf1N1B4_5043 at 5589.119 kb on + strand, within Pf1N1B4_5043 at 5589.120 kb on - strand, within Pf1N1B4_5043 at 5589.120 kb on - strand, within Pf1N1B4_5043 at 5589.148 kb on - strand, within Pf1N1B4_5043 at 5589.299 kb on - strand at 5589.299 kb on - strand at 5589.328 kb on + strand at 5589.329 kb on - strand at 5589.450 kb on + strand at 5589.548 kb on - strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.635 kb on - strand at 5589.635 kb on - strand at 5589.775 kb on + strand at 5589.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 5,585,117 + -0.6 5,585,117 + -0.1 5,585,117 + +0.2 5,585,135 + +0.6 5,585,135 + +1.0 5,585,135 + -0.4 5,585,136 - +0.2 5,585,136 - +1.2 5,585,270 + +0.2 5,585,270 + -0.0 5,585,271 - +0.2 5,585,271 - -0.2 5,585,422 - Pf1N1B4_5040 0.38 -0.5 5,585,519 + Pf1N1B4_5040 0.60 +0.9 5,585,520 - Pf1N1B4_5040 0.60 -0.4 5,585,579 - Pf1N1B4_5040 0.74 -2.0 5,585,579 - Pf1N1B4_5040 0.74 -0.0 5,585,579 - Pf1N1B4_5040 0.74 -0.1 5,585,579 - Pf1N1B4_5040 0.74 +1.4 5,585,579 - Pf1N1B4_5040 0.74 +0.2 5,585,838 + Pf1N1B4_5041 0.29 -0.4 5,585,839 - Pf1N1B4_5041 0.29 -2.4 5,585,839 - Pf1N1B4_5041 0.29 -1.2 5,585,839 - Pf1N1B4_5041 0.29 -2.4 5,585,839 - Pf1N1B4_5041 0.29 -1.0 5,585,937 + Pf1N1B4_5041 0.55 +1.3 5,585,938 - Pf1N1B4_5041 0.55 -0.1 5,586,437 + -0.4 5,586,437 + Pf1N1B4_5042 0.12 -2.4 5,586,438 - Pf1N1B4_5042 0.12 -0.9 5,586,542 + Pf1N1B4_5042 0.16 -1.4 5,586,876 - Pf1N1B4_5042 0.28 -1.4 5,586,962 + Pf1N1B4_5042 0.31 -2.0 5,586,962 + Pf1N1B4_5042 0.31 -0.8 5,586,962 + Pf1N1B4_5042 0.31 -0.7 5,586,962 + Pf1N1B4_5042 0.31 -1.4 5,587,169 + Pf1N1B4_5042 0.39 -2.4 5,587,187 + Pf1N1B4_5042 0.40 -2.0 5,587,497 - Pf1N1B4_5042 0.51 -3.0 5,587,517 + Pf1N1B4_5042 0.52 -0.8 5,587,517 + -0.4 5,587,517 + -1.4 5,587,517 + Pf1N1B4_5042 0.52 -0.4 5,587,517 + Pf1N1B4_5042 0.52 -1.3 5,587,518 - Pf1N1B4_5042 0.52 -1.4 5,587,826 + Pf1N1B4_5042 0.63 -1.4 5,587,827 - Pf1N1B4_5042 0.64 +0.2 5,588,427 + Pf1N1B4_5042 0.86 -2.0 5,588,676 + -0.8 5,588,676 + -2.4 5,588,704 + -0.4 5,589,046 + -4.0 5,589,096 + +0.4 5,589,096 + +0.6 5,589,097 - +1.4 5,589,119 + Pf1N1B4_5043 0.14 -1.0 5,589,119 + Pf1N1B4_5043 0.14 -0.0 5,589,119 + Pf1N1B4_5043 0.14 +1.4 5,589,120 - Pf1N1B4_5043 0.14 -0.8 5,589,120 - Pf1N1B4_5043 0.14 -0.1 5,589,148 - Pf1N1B4_5043 0.36 -1.4 5,589,299 - -0.0 5,589,299 - +0.9 5,589,328 + +0.2 5,589,329 - -0.4 5,589,450 + -0.1 5,589,548 - -0.1 5,589,634 + +1.6 5,589,634 + -1.4 5,589,634 + +0.2 5,589,635 - +0.4 5,589,635 - +0.2 5,589,775 + -0.4 5,589,775 + +0.6
Or see this region's nucleotide sequence