Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5042

Experiment: nitrate (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5039 and Pf1N1B4_5040 are separated by 86 nucleotidesPf1N1B4_5040 and Pf1N1B4_5041 are separated by 31 nucleotidesPf1N1B4_5041 and Pf1N1B4_5042 overlap by 1 nucleotidesPf1N1B4_5042 and Pf1N1B4_5043 are separated by 289 nucleotidesPf1N1B4_5043 and Pf1N1B4_5044 are separated by 402 nucleotides Pf1N1B4_5039 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,584,394 to 5,585,167 _5039 Pf1N1B4_5040 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 5,585,254 to 5,585,694 _5040 Pf1N1B4_5041 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 5,585,726 to 5,586,112 _5041 Pf1N1B4_5042 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 5,586,112 to 5,588,811 _5042 Pf1N1B4_5043 - hypothetical protein, at 5,589,101 to 5,589,232 _5043 Pf1N1B4_5044 - hypothetical protein, at 5,589,635 to 5,589,994 _5044 Position (kb) 5586 5587 5588 5589Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5585.117 kb on + strandat 5585.117 kb on + strandat 5585.117 kb on + strandat 5585.135 kb on + strandat 5585.135 kb on + strandat 5585.135 kb on + strandat 5585.136 kb on - strandat 5585.136 kb on - strandat 5585.270 kb on + strandat 5585.270 kb on + strandat 5585.271 kb on - strandat 5585.271 kb on - strandat 5585.422 kb on - strand, within Pf1N1B4_5040at 5585.519 kb on + strand, within Pf1N1B4_5040at 5585.520 kb on - strand, within Pf1N1B4_5040at 5585.579 kb on - strand, within Pf1N1B4_5040at 5585.579 kb on - strand, within Pf1N1B4_5040at 5585.579 kb on - strand, within Pf1N1B4_5040at 5585.579 kb on - strand, within Pf1N1B4_5040at 5585.579 kb on - strand, within Pf1N1B4_5040at 5585.838 kb on + strand, within Pf1N1B4_5041at 5585.839 kb on - strand, within Pf1N1B4_5041at 5585.839 kb on - strand, within Pf1N1B4_5041at 5585.839 kb on - strand, within Pf1N1B4_5041at 5585.839 kb on - strand, within Pf1N1B4_5041at 5585.937 kb on + strand, within Pf1N1B4_5041at 5585.938 kb on - strand, within Pf1N1B4_5041at 5586.437 kb on + strandat 5586.437 kb on + strand, within Pf1N1B4_5042at 5586.438 kb on - strand, within Pf1N1B4_5042at 5586.542 kb on + strand, within Pf1N1B4_5042at 5586.876 kb on - strand, within Pf1N1B4_5042at 5586.962 kb on + strand, within Pf1N1B4_5042at 5586.962 kb on + strand, within Pf1N1B4_5042at 5586.962 kb on + strand, within Pf1N1B4_5042at 5586.962 kb on + strand, within Pf1N1B4_5042at 5587.169 kb on + strand, within Pf1N1B4_5042at 5587.187 kb on + strand, within Pf1N1B4_5042at 5587.497 kb on - strand, within Pf1N1B4_5042at 5587.517 kb on + strand, within Pf1N1B4_5042at 5587.517 kb on + strandat 5587.517 kb on + strandat 5587.517 kb on + strand, within Pf1N1B4_5042at 5587.517 kb on + strand, within Pf1N1B4_5042at 5587.518 kb on - strand, within Pf1N1B4_5042at 5587.826 kb on + strand, within Pf1N1B4_5042at 5587.827 kb on - strand, within Pf1N1B4_5042at 5588.427 kb on + strand, within Pf1N1B4_5042at 5588.676 kb on + strandat 5588.676 kb on + strandat 5588.704 kb on + strandat 5589.046 kb on + strandat 5589.096 kb on + strandat 5589.096 kb on + strandat 5589.097 kb on - strandat 5589.119 kb on + strand, within Pf1N1B4_5043at 5589.119 kb on + strand, within Pf1N1B4_5043at 5589.119 kb on + strand, within Pf1N1B4_5043at 5589.120 kb on - strand, within Pf1N1B4_5043at 5589.120 kb on - strand, within Pf1N1B4_5043at 5589.148 kb on - strand, within Pf1N1B4_5043at 5589.299 kb on - strandat 5589.299 kb on - strandat 5589.328 kb on + strandat 5589.329 kb on - strandat 5589.450 kb on + strandat 5589.548 kb on - strandat 5589.634 kb on + strandat 5589.634 kb on + strandat 5589.634 kb on + strandat 5589.635 kb on - strandat 5589.635 kb on - strandat 5589.775 kb on + strandat 5589.775 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate (N)
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5,585,117 + -0.6
5,585,117 + -0.1
5,585,117 + +0.2
5,585,135 + +0.6
5,585,135 + +1.0
5,585,135 + -0.4
5,585,136 - +0.2
5,585,136 - +1.2
5,585,270 + +0.2
5,585,270 + -0.0
5,585,271 - +0.2
5,585,271 - -0.2
5,585,422 - Pf1N1B4_5040 0.38 -0.5
5,585,519 + Pf1N1B4_5040 0.60 +0.9
5,585,520 - Pf1N1B4_5040 0.60 -0.4
5,585,579 - Pf1N1B4_5040 0.74 -2.0
5,585,579 - Pf1N1B4_5040 0.74 -0.0
5,585,579 - Pf1N1B4_5040 0.74 -0.1
5,585,579 - Pf1N1B4_5040 0.74 +1.4
5,585,579 - Pf1N1B4_5040 0.74 +0.2
5,585,838 + Pf1N1B4_5041 0.29 -0.4
5,585,839 - Pf1N1B4_5041 0.29 -2.4
5,585,839 - Pf1N1B4_5041 0.29 -1.2
5,585,839 - Pf1N1B4_5041 0.29 -2.4
5,585,839 - Pf1N1B4_5041 0.29 -1.0
5,585,937 + Pf1N1B4_5041 0.55 +1.3
5,585,938 - Pf1N1B4_5041 0.55 -0.1
5,586,437 + -0.4
5,586,437 + Pf1N1B4_5042 0.12 -2.4
5,586,438 - Pf1N1B4_5042 0.12 -0.9
5,586,542 + Pf1N1B4_5042 0.16 -1.4
5,586,876 - Pf1N1B4_5042 0.28 -1.4
5,586,962 + Pf1N1B4_5042 0.31 -2.0
5,586,962 + Pf1N1B4_5042 0.31 -0.8
5,586,962 + Pf1N1B4_5042 0.31 -0.7
5,586,962 + Pf1N1B4_5042 0.31 -1.4
5,587,169 + Pf1N1B4_5042 0.39 -2.4
5,587,187 + Pf1N1B4_5042 0.40 -2.0
5,587,497 - Pf1N1B4_5042 0.51 -3.0
5,587,517 + Pf1N1B4_5042 0.52 -0.8
5,587,517 + -0.4
5,587,517 + -1.4
5,587,517 + Pf1N1B4_5042 0.52 -0.4
5,587,517 + Pf1N1B4_5042 0.52 -1.3
5,587,518 - Pf1N1B4_5042 0.52 -1.4
5,587,826 + Pf1N1B4_5042 0.63 -1.4
5,587,827 - Pf1N1B4_5042 0.64 +0.2
5,588,427 + Pf1N1B4_5042 0.86 -2.0
5,588,676 + -0.8
5,588,676 + -2.4
5,588,704 + -0.4
5,589,046 + -4.0
5,589,096 + +0.4
5,589,096 + +0.6
5,589,097 - +1.4
5,589,119 + Pf1N1B4_5043 0.14 -1.0
5,589,119 + Pf1N1B4_5043 0.14 -0.0
5,589,119 + Pf1N1B4_5043 0.14 +1.4
5,589,120 - Pf1N1B4_5043 0.14 -0.8
5,589,120 - Pf1N1B4_5043 0.14 -0.1
5,589,148 - Pf1N1B4_5043 0.36 -1.4
5,589,299 - -0.0
5,589,299 - +0.9
5,589,328 + +0.2
5,589,329 - -0.4
5,589,450 + -0.1
5,589,548 - -0.1
5,589,634 + +1.6
5,589,634 + -1.4
5,589,634 + +0.2
5,589,635 - +0.4
5,589,635 - +0.2
5,589,775 + -0.4
5,589,775 + +0.6

Or see this region's nucleotide sequence