Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1848 and Pf1N1B4_1849 are separated by 244 nucleotides Pf1N1B4_1849 and Pf1N1B4_1850 are separated by 84 nucleotides
Pf1N1B4_1848 - Radical SAM domain protein, at 2,120,038 to 2,121,096
_1848
Pf1N1B4_1849 - Carbonic anhydrase (EC 4.2.1.1), at 2,121,341 to 2,122,072
_1849
Pf1N1B4_1850 - Sulfate transporter family protein in cluster with carbonic anhydrase, at 2,122,157 to 2,123,683
_1850
Position (kb)
2121
2122
2123 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2120.437 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.472 kb on + strand, within Pf1N1B4_1848 at 2120.524 kb on - strand, within Pf1N1B4_1848 at 2120.524 kb on - strand, within Pf1N1B4_1848 at 2120.591 kb on + strand, within Pf1N1B4_1848 at 2120.632 kb on - strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.806 kb on + strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.807 kb on - strand, within Pf1N1B4_1848 at 2120.929 kb on + strand, within Pf1N1B4_1848 at 2120.929 kb on + strand, within Pf1N1B4_1848 at 2120.929 kb on + strand, within Pf1N1B4_1848 at 2120.930 kb on - strand, within Pf1N1B4_1848 at 2120.930 kb on - strand, within Pf1N1B4_1848 at 2121.094 kb on + strand at 2121.094 kb on + strand at 2121.095 kb on - strand at 2121.110 kb on + strand at 2121.120 kb on + strand at 2121.163 kb on + strand at 2121.172 kb on + strand at 2121.181 kb on + strand at 2121.181 kb on + strand at 2121.181 kb on + strand at 2121.181 kb on + strand at 2121.192 kb on + strand at 2121.206 kb on + strand at 2121.207 kb on - strand at 2121.207 kb on - strand at 2121.221 kb on - strand at 2121.274 kb on + strand at 2121.274 kb on + strand at 2121.424 kb on + strand, within Pf1N1B4_1849 at 2121.424 kb on + strand, within Pf1N1B4_1849 at 2121.623 kb on + strand, within Pf1N1B4_1849 at 2121.662 kb on + strand, within Pf1N1B4_1849 at 2121.685 kb on - strand, within Pf1N1B4_1849 at 2121.962 kb on + strand, within Pf1N1B4_1849 at 2121.962 kb on + strand, within Pf1N1B4_1849 at 2121.963 kb on - strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.992 kb on + strand, within Pf1N1B4_1849 at 2121.993 kb on - strand, within Pf1N1B4_1849 at 2121.993 kb on - strand, within Pf1N1B4_1849 at 2122.070 kb on + strand at 2122.070 kb on + strand at 2122.070 kb on + strand at 2122.070 kb on + strand at 2122.070 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.080 kb on + strand at 2122.081 kb on - strand at 2122.081 kb on - strand at 2122.081 kb on - strand at 2122.081 kb on - strand at 2122.081 kb on - strand at 2122.081 kb on - strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.084 kb on + strand at 2122.085 kb on - strand at 2122.085 kb on - strand at 2122.173 kb on + strand at 2122.174 kb on - strand at 2122.174 kb on - strand at 2122.174 kb on - strand at 2122.174 kb on - strand at 2122.374 kb on + strand, within Pf1N1B4_1850 at 2122.375 kb on - strand, within Pf1N1B4_1850 at 2122.375 kb on - strand, within Pf1N1B4_1850 at 2122.509 kb on - strand, within Pf1N1B4_1850 at 2122.743 kb on + strand, within Pf1N1B4_1850 at 2122.744 kb on - strand, within Pf1N1B4_1850 at 2122.842 kb on + strand, within Pf1N1B4_1850 at 2122.954 kb on + strand, within Pf1N1B4_1850 at 2122.954 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.007 kb on + strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.008 kb on - strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.011 kb on + strand, within Pf1N1B4_1850 at 2123.012 kb on - strand, within Pf1N1B4_1850 at 2123.012 kb on - strand, within Pf1N1B4_1850 at 2123.012 kb on - strand, within Pf1N1B4_1850 at 2123.012 kb on - strand, within Pf1N1B4_1850 at 2123.012 kb on - strand, within Pf1N1B4_1850
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 2,120,437 + Pf1N1B4_1848 0.38 +1.6 2,120,472 + Pf1N1B4_1848 0.41 +0.0 2,120,472 + Pf1N1B4_1848 0.41 +0.1 2,120,472 + Pf1N1B4_1848 0.41 -0.1 2,120,524 - Pf1N1B4_1848 0.46 -0.4 2,120,524 - Pf1N1B4_1848 0.46 -0.4 2,120,591 + Pf1N1B4_1848 0.52 +0.1 2,120,632 - Pf1N1B4_1848 0.56 -0.1 2,120,806 + Pf1N1B4_1848 0.73 -0.4 2,120,806 + Pf1N1B4_1848 0.73 +0.6 2,120,806 + Pf1N1B4_1848 0.73 +0.1 2,120,806 + Pf1N1B4_1848 0.73 +0.6 2,120,806 + Pf1N1B4_1848 0.73 +0.9 2,120,806 + Pf1N1B4_1848 0.73 +1.4 2,120,806 + Pf1N1B4_1848 0.73 -0.0 2,120,806 + Pf1N1B4_1848 0.73 -0.4 2,120,806 + Pf1N1B4_1848 0.73 -0.0 2,120,806 + Pf1N1B4_1848 0.73 +0.1 2,120,806 + Pf1N1B4_1848 0.73 -0.4 2,120,807 - Pf1N1B4_1848 0.73 +0.6 2,120,807 - Pf1N1B4_1848 0.73 +1.4 2,120,807 - Pf1N1B4_1848 0.73 -1.4 2,120,807 - Pf1N1B4_1848 0.73 +2.3 2,120,807 - Pf1N1B4_1848 0.73 -1.2 2,120,807 - Pf1N1B4_1848 0.73 +1.2 2,120,807 - Pf1N1B4_1848 0.73 -0.2 2,120,807 - Pf1N1B4_1848 0.73 +1.9 2,120,929 + Pf1N1B4_1848 0.84 +0.0 2,120,929 + Pf1N1B4_1848 0.84 -1.0 2,120,929 + Pf1N1B4_1848 0.84 +0.2 2,120,930 - Pf1N1B4_1848 0.84 +0.1 2,120,930 - Pf1N1B4_1848 0.84 -0.4 2,121,094 + +0.1 2,121,094 + -0.7 2,121,095 - +2.6 2,121,110 + +0.3 2,121,120 + -0.4 2,121,163 + +1.9 2,121,172 + -0.0 2,121,181 + +1.0 2,121,181 + -1.5 2,121,181 + +0.9 2,121,181 + -0.5 2,121,192 + -0.1 2,121,206 + -0.3 2,121,207 - -0.8 2,121,207 - +0.3 2,121,221 - +0.1 2,121,274 + -0.7 2,121,274 + -1.2 2,121,424 + Pf1N1B4_1849 0.11 +1.1 2,121,424 + Pf1N1B4_1849 0.11 -1.5 2,121,623 + Pf1N1B4_1849 0.39 +0.3 2,121,662 + Pf1N1B4_1849 0.44 +1.0 2,121,685 - Pf1N1B4_1849 0.47 +1.1 2,121,962 + Pf1N1B4_1849 0.85 -0.5 2,121,962 + Pf1N1B4_1849 0.85 +0.9 2,121,963 - Pf1N1B4_1849 0.85 -0.2 2,121,992 + Pf1N1B4_1849 0.89 +0.3 2,121,992 + Pf1N1B4_1849 0.89 -1.5 2,121,992 + Pf1N1B4_1849 0.89 -0.2 2,121,992 + Pf1N1B4_1849 0.89 +0.9 2,121,992 + Pf1N1B4_1849 0.89 +1.5 2,121,992 + Pf1N1B4_1849 0.89 -1.0 2,121,992 + Pf1N1B4_1849 0.89 -0.9 2,121,993 - Pf1N1B4_1849 0.89 -0.5 2,121,993 - Pf1N1B4_1849 0.89 +1.1 2,122,070 + -0.5 2,122,070 + +0.5 2,122,070 + -0.5 2,122,070 + +0.5 2,122,070 + +1.0 2,122,080 + +2.5 2,122,080 + -0.0 2,122,080 + -0.5 2,122,080 + +1.7 2,122,080 + -0.3 2,122,080 + -0.0 2,122,080 + +0.5 2,122,080 + +0.2 2,122,080 + +1.1 2,122,080 + +0.9 2,122,080 + +0.9 2,122,080 + +0.3 2,122,080 + -0.9 2,122,080 + +0.5 2,122,080 + +1.1 2,122,080 + +2.0 2,122,080 + +0.5 2,122,080 + +0.8 2,122,080 + +0.5 2,122,081 - -0.2 2,122,081 - -0.5 2,122,081 - -0.5 2,122,081 - -0.5 2,122,081 - +1.1 2,122,081 - -0.9 2,122,084 + +1.7 2,122,084 + +0.0 2,122,084 + +0.3 2,122,084 + +1.3 2,122,084 + -0.9 2,122,084 + +0.1 2,122,084 + +0.3 2,122,084 + -0.5 2,122,085 - -0.5 2,122,085 - -0.5 2,122,173 + +0.5 2,122,174 - -0.5 2,122,174 - -0.5 2,122,174 - -2.0 2,122,174 - +1.3 2,122,374 + Pf1N1B4_1850 0.14 +0.1 2,122,375 - Pf1N1B4_1850 0.14 -0.5 2,122,375 - Pf1N1B4_1850 0.14 +0.8 2,122,509 - Pf1N1B4_1850 0.23 +1.1 2,122,743 + Pf1N1B4_1850 0.38 +0.3 2,122,744 - Pf1N1B4_1850 0.38 +0.5 2,122,842 + Pf1N1B4_1850 0.45 +1.9 2,122,954 + Pf1N1B4_1850 0.52 -0.5 2,122,954 + Pf1N1B4_1850 0.52 +1.3 2,123,007 + Pf1N1B4_1850 0.56 +1.0 2,123,007 + Pf1N1B4_1850 0.56 -0.5 2,123,007 + Pf1N1B4_1850 0.56 +0.9 2,123,007 + Pf1N1B4_1850 0.56 +0.4 2,123,007 + Pf1N1B4_1850 0.56 -1.0 2,123,007 + Pf1N1B4_1850 0.56 +0.1 2,123,007 + Pf1N1B4_1850 0.56 +0.7 2,123,008 - Pf1N1B4_1850 0.56 +2.3 2,123,008 - Pf1N1B4_1850 0.56 +1.7 2,123,008 - Pf1N1B4_1850 0.56 +0.1 2,123,008 - Pf1N1B4_1850 0.56 +1.5 2,123,008 - Pf1N1B4_1850 0.56 -0.5 2,123,008 - Pf1N1B4_1850 0.56 -0.5 2,123,008 - Pf1N1B4_1850 0.56 +1.9 2,123,011 + Pf1N1B4_1850 0.56 -0.1 2,123,011 + Pf1N1B4_1850 0.56 -0.5 2,123,011 + Pf1N1B4_1850 0.56 -0.8 2,123,011 + Pf1N1B4_1850 0.56 +2.3 2,123,011 + Pf1N1B4_1850 0.56 +0.5 2,123,011 + Pf1N1B4_1850 0.56 +1.5 2,123,011 + Pf1N1B4_1850 0.56 +1.1 2,123,012 - Pf1N1B4_1850 0.56 +0.5 2,123,012 - Pf1N1B4_1850 0.56 -1.0 2,123,012 - Pf1N1B4_1850 0.56 +1.1 2,123,012 - Pf1N1B4_1850 0.56 -0.5 2,123,012 - Pf1N1B4_1850 0.56 +0.5
Or see this region's nucleotide sequence