Experiment: ALP with Thallium(I) acetate 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0082 and Dsui_0083 are separated by 2 nucleotides Dsui_0083 and Dsui_0084 are separated by 10 nucleotides
Dsui_0082: Dsui_0082 - UDP-4-keto-6-deoxy-N-acetylglucosamine 4-aminotransferase, at 79,093 to 80,253
_0082
Dsui_0083: Dsui_0083 - UDP-N-acetylglucosamine 4,6-dehydratase, at 80,256 to 81,254
_0083
Dsui_0084: Dsui_0084 - ABC-type bacteriocin/lantibiotic exporter with N-terminal double-glycine peptidase domain, at 81,265 to 83,040
_0084
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 80.295 kb on + strand at 80.298 kb on - strand at 80.344 kb on - strand at 80.346 kb on + strand at 80.346 kb on + strand at 80.353 kb on - strand at 80.354 kb on - strand at 80.354 kb on - strand at 80.413 kb on + strand, within Dsui_0083 at 80.422 kb on + strand, within Dsui_0083 at 80.459 kb on - strand, within Dsui_0083 at 80.470 kb on + strand, within Dsui_0083 at 80.472 kb on + strand, within Dsui_0083 at 80.472 kb on - strand, within Dsui_0083 at 80.534 kb on - strand, within Dsui_0083 at 80.550 kb on - strand, within Dsui_0083 at 80.561 kb on + strand, within Dsui_0083 at 80.615 kb on + strand, within Dsui_0083 at 80.628 kb on - strand, within Dsui_0083 at 80.628 kb on - strand, within Dsui_0083 at 80.630 kb on + strand, within Dsui_0083 at 80.630 kb on + strand, within Dsui_0083 at 80.642 kb on + strand, within Dsui_0083 at 80.642 kb on + strand, within Dsui_0083 at 80.664 kb on + strand, within Dsui_0083 at 80.690 kb on + strand, within Dsui_0083 at 80.708 kb on - strand, within Dsui_0083 at 80.730 kb on - strand, within Dsui_0083 at 80.845 kb on + strand, within Dsui_0083 at 80.860 kb on - strand, within Dsui_0083 at 80.863 kb on + strand, within Dsui_0083 at 80.863 kb on + strand, within Dsui_0083 at 80.871 kb on - strand, within Dsui_0083 at 80.887 kb on + strand, within Dsui_0083 at 80.888 kb on - strand, within Dsui_0083 at 80.931 kb on - strand, within Dsui_0083 at 80.976 kb on + strand, within Dsui_0083 at 80.976 kb on + strand, within Dsui_0083 at 80.979 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.986 kb on + strand, within Dsui_0083 at 81.010 kb on + strand, within Dsui_0083 at 81.010 kb on + strand, within Dsui_0083 at 81.033 kb on + strand, within Dsui_0083 at 81.166 kb on + strand at 81.172 kb on + strand at 81.174 kb on - strand at 81.174 kb on - strand at 81.251 kb on + strand at 81.290 kb on - strand at 81.322 kb on - strand at 81.374 kb on - strand at 81.392 kb on + strand at 81.395 kb on + strand at 81.403 kb on - strand at 81.490 kb on - strand, within Dsui_0084 at 81.490 kb on - strand, within Dsui_0084 at 81.492 kb on - strand, within Dsui_0084 at 81.492 kb on - strand, within Dsui_0084 at 81.583 kb on - strand, within Dsui_0084 at 81.712 kb on - strand, within Dsui_0084 at 81.765 kb on + strand, within Dsui_0084 at 81.799 kb on + strand, within Dsui_0084 at 81.817 kb on + strand, within Dsui_0084 at 81.825 kb on - strand, within Dsui_0084 at 81.859 kb on - strand, within Dsui_0084 at 81.893 kb on + strand, within Dsui_0084 at 81.976 kb on + strand, within Dsui_0084 at 81.998 kb on + strand, within Dsui_0084 at 82.012 kb on - strand, within Dsui_0084 at 82.115 kb on + strand, within Dsui_0084 at 82.178 kb on - strand, within Dsui_0084 at 82.183 kb on - strand, within Dsui_0084 at 82.188 kb on + strand, within Dsui_0084 at 82.207 kb on + strand, within Dsui_0084
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Thallium(I) acetate 0.004 mg/ml remove 80,295 + +1.0 80,298 - +0.4 80,344 - -5.0 80,346 + +1.0 80,346 + +1.0 80,353 - -3.9 80,354 - -3.8 80,354 - -4.3 80,413 + Dsui_0083 0.16 +1.0 80,422 + Dsui_0083 0.17 -0.6 80,459 - Dsui_0083 0.20 -4.5 80,470 + Dsui_0083 0.21 +1.0 80,472 + Dsui_0083 0.22 -1.6 80,472 - Dsui_0083 0.22 -5.6 80,534 - Dsui_0083 0.28 -4.6 80,550 - Dsui_0083 0.29 -4.5 80,561 + Dsui_0083 0.31 -0.6 80,615 + Dsui_0083 0.36 -1.4 80,628 - Dsui_0083 0.37 -5.5 80,628 - Dsui_0083 0.37 -4.4 80,630 + Dsui_0083 0.37 +1.0 80,630 + Dsui_0083 0.37 -0.6 80,642 + Dsui_0083 0.39 +1.0 80,642 + Dsui_0083 0.39 -1.4 80,664 + Dsui_0083 0.41 +1.0 80,690 + Dsui_0083 0.43 -1.4 80,708 - Dsui_0083 0.45 -4.2 80,730 - Dsui_0083 0.47 -4.7 80,845 + Dsui_0083 0.59 +1.0 80,860 - Dsui_0083 0.60 -3.7 80,863 + Dsui_0083 0.61 -0.6 80,863 + Dsui_0083 0.61 +1.0 80,871 - Dsui_0083 0.62 -3.5 80,887 + Dsui_0083 0.63 -0.0 80,888 - Dsui_0083 0.63 -3.9 80,931 - Dsui_0083 0.68 -4.5 80,976 + Dsui_0083 0.72 +1.0 80,976 + Dsui_0083 0.72 -0.6 80,979 - Dsui_0083 0.72 -3.7 80,984 - Dsui_0083 0.73 -4.4 80,984 - Dsui_0083 0.73 -4.2 80,984 - Dsui_0083 0.73 -4.2 80,984 - Dsui_0083 0.73 -4.3 80,984 - Dsui_0083 0.73 -4.0 80,986 + Dsui_0083 0.73 +1.0 81,010 + Dsui_0083 0.75 +1.0 81,010 + Dsui_0083 0.75 +1.0 81,033 + Dsui_0083 0.78 +1.0 81,166 + +1.0 81,172 + -2.7 81,174 - -4.8 81,174 - -4.9 81,251 + +1.0 81,290 - -0.0 81,322 - +0.2 81,374 - -4.9 81,392 + -0.6 81,395 + -0.0 81,403 - -4.2 81,490 - Dsui_0084 0.13 -3.7 81,490 - Dsui_0084 0.13 -4.5 81,492 - Dsui_0084 0.13 -6.1 81,492 - Dsui_0084 0.13 -5.9 81,583 - Dsui_0084 0.18 -5.3 81,712 - Dsui_0084 0.25 -6.1 81,765 + Dsui_0084 0.28 -2.2 81,799 + Dsui_0084 0.30 -0.6 81,817 + Dsui_0084 0.31 -1.5 81,825 - Dsui_0084 0.32 -5.8 81,859 - Dsui_0084 0.33 -4.6 81,893 + Dsui_0084 0.35 -1.0 81,976 + Dsui_0084 0.40 -2.8 81,998 + Dsui_0084 0.41 -1.6 82,012 - Dsui_0084 0.42 -5.4 82,115 + Dsui_0084 0.48 -0.6 82,178 - Dsui_0084 0.51 -5.6 82,183 - Dsui_0084 0.52 -5.8 82,188 + Dsui_0084 0.52 -0.0 82,207 + Dsui_0084 0.53 +1.0
Or see this region's nucleotide sequence