Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16950 and BWI76_RS16955 are separated by 26 nucleotides BWI76_RS16955 and BWI76_RS16960 are separated by 509 nucleotides BWI76_RS16960 and BWI76_RS16965 are separated by 311 nucleotides BWI76_RS16965 and BWI76_RS16970 are separated by 64 nucleotides
BWI76_RS16950: BWI76_RS16950 - TetR family transcriptional regulator, at 3,516,175 to 3,516,813
_RS16950
BWI76_RS16955: BWI76_RS16955 - hypothetical protein, at 3,516,840 to 3,517,052
_RS16955
BWI76_RS16960: BWI76_RS16960 - pyruvate kinase, at 3,517,562 to 3,518,974
_RS16960
BWI76_RS16965: BWI76_RS16965 - murein lipoprotein, at 3,519,286 to 3,519,522
_RS16965
BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570
_RS16970
Position (kb)
3517
3518
3519 Strain fitness (log2 ratio)
-1
0
1 at 3516.965 kb on + strand, within BWI76_RS16955 at 3517.113 kb on + strand at 3517.121 kb on - strand at 3517.895 kb on + strand, within BWI76_RS16960 at 3519.241 kb on + strand at 3519.249 kb on + strand at 3519.266 kb on - strand at 3519.268 kb on + strand at 3519.276 kb on - strand at 3519.293 kb on + strand at 3519.326 kb on + strand, within BWI76_RS16965 at 3519.481 kb on + strand, within BWI76_RS16965 at 3519.692 kb on - strand, within BWI76_RS16970 at 3519.769 kb on - strand, within BWI76_RS16970 at 3519.776 kb on - strand, within BWI76_RS16970
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 3,516,965 + BWI76_RS16955 0.59 -1.0 3,517,113 + +0.2 3,517,121 - +0.4 3,517,895 + BWI76_RS16960 0.24 +0.4 3,519,241 + -1.2 3,519,249 + -1.4 3,519,266 - +0.0 3,519,268 + -0.7 3,519,276 - -1.4 3,519,293 + -1.1 3,519,326 + BWI76_RS16965 0.17 -1.6 3,519,481 + BWI76_RS16965 0.82 -1.6 3,519,692 - BWI76_RS16970 0.11 -0.1 3,519,769 - BWI76_RS16970 0.18 +0.3 3,519,776 - BWI76_RS16970 0.19 +0.5
Or see this region's nucleotide sequence