Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32295 and AZOBR_RS32300 are separated by 14 nucleotides AZOBR_RS32300 and AZOBR_RS32305 are separated by 27 nucleotides AZOBR_RS32305 and AZOBR_RS32310 overlap by 4 nucleotides AZOBR_RS32310 and AZOBR_RS32315 are separated by 157 nucleotides
AZOBR_RS32295: AZOBR_RS32295 - Asp/Glu racemase, at 613,572 to 614,327
_RS32295
AZOBR_RS32300: AZOBR_RS32300 - alpha/beta hydrolase, at 614,342 to 615,151
_RS32300
AZOBR_RS32305: AZOBR_RS32305 - hypothetical protein, at 615,179 to 616,225
_RS32305
AZOBR_RS32310: AZOBR_RS32310 - N-carbamoylsarcosine amidase, at 616,222 to 616,836
_RS32310
AZOBR_RS32315: AZOBR_RS32315 - TetR family transcriptional regulator, at 616,994 to 617,692
_RS32315
Position (kb) 615
616
617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 614.253 kb on + strand at 614.254 kb on - strand at 614.254 kb on - strand at 614.322 kb on + strand at 614.322 kb on + strand at 614.323 kb on - strand at 614.336 kb on + strand at 614.336 kb on + strand at 614.337 kb on - strand at 614.402 kb on + strand at 614.402 kb on + strand at 614.402 kb on + strand at 614.403 kb on - strand at 614.403 kb on - strand at 614.513 kb on + strand, within AZOBR_RS32300 at 614.513 kb on + strand, within AZOBR_RS32300 at 614.513 kb on + strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.514 kb on - strand, within AZOBR_RS32300 at 614.630 kb on + strand, within AZOBR_RS32300 at 614.630 kb on + strand, within AZOBR_RS32300 at 614.775 kb on - strand, within AZOBR_RS32300 at 615.113 kb on + strand at 615.113 kb on + strand at 615.113 kb on + strand at 615.114 kb on - strand at 615.114 kb on - strand at 615.199 kb on + strand at 615.710 kb on + strand, within AZOBR_RS32305 at 615.711 kb on - strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.842 kb on + strand, within AZOBR_RS32305 at 615.843 kb on - strand, within AZOBR_RS32305 at 615.929 kb on + strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.930 kb on - strand, within AZOBR_RS32305 at 615.960 kb on - strand, within AZOBR_RS32305 at 616.247 kb on - strand at 616.247 kb on - strand at 616.247 kb on - strand at 616.795 kb on + strand at 617.100 kb on - strand, within AZOBR_RS32315 at 617.100 kb on - strand, within AZOBR_RS32315 at 617.166 kb on - strand, within AZOBR_RS32315
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days remove 614,253 + -1.6 614,254 - +0.3 614,254 - +0.4 614,322 + +0.2 614,322 + +1.0 614,323 - +0.3 614,336 + +2.1 614,336 + +0.8 614,337 - -0.4 614,402 + +0.4 614,402 + +0.2 614,402 + +0.9 614,403 - +0.2 614,403 - -3.3 614,513 + AZOBR_RS32300 0.21 +1.1 614,513 + AZOBR_RS32300 0.21 -1.8 614,513 + AZOBR_RS32300 0.21 +0.1 614,514 - AZOBR_RS32300 0.21 +0.4 614,514 - AZOBR_RS32300 0.21 +1.8 614,514 - AZOBR_RS32300 0.21 -0.1 614,514 - AZOBR_RS32300 0.21 -0.3 614,514 - AZOBR_RS32300 0.21 -0.9 614,630 + AZOBR_RS32300 0.36 +0.1 614,630 + AZOBR_RS32300 0.36 +0.0 614,775 - AZOBR_RS32300 0.53 +1.6 615,113 + +0.1 615,113 + -1.0 615,113 + +1.3 615,114 - -0.4 615,114 - -0.0 615,199 + -1.6 615,710 + AZOBR_RS32305 0.51 -0.9 615,711 - AZOBR_RS32305 0.51 -0.3 615,842 + AZOBR_RS32305 0.63 -0.5 615,842 + AZOBR_RS32305 0.63 -1.4 615,842 + AZOBR_RS32305 0.63 +0.5 615,843 - AZOBR_RS32305 0.63 +1.3 615,929 + AZOBR_RS32305 0.72 -0.1 615,930 - AZOBR_RS32305 0.72 -2.2 615,930 - AZOBR_RS32305 0.72 -0.7 615,930 - AZOBR_RS32305 0.72 -0.6 615,960 - AZOBR_RS32305 0.75 +0.5 616,247 - -1.3 616,247 - +1.2 616,247 - +0.6 616,795 + -0.1 617,100 - AZOBR_RS32315 0.15 +0.3 617,100 - AZOBR_RS32315 0.15 -0.3 617,166 - AZOBR_RS32315 0.25 -0.2
Or see this region's nucleotide sequence