Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS10240 and AZOBR_RS10245 are separated by 134 nucleotides AZOBR_RS10245 and AZOBR_RS10250 are separated by 268 nucleotides AZOBR_RS10250 and AZOBR_RS10255 overlap by 26 nucleotides
AZOBR_RS10240: AZOBR_RS10240 - L-threonine-O-3-phosphate decarboxylase, at 2,139,433 to 2,140,452
_RS10240
AZOBR_RS10245: AZOBR_RS10245 - diguanylate cyclase, at 2,140,587 to 2,142,209
_RS10245
AZOBR_RS10250: AZOBR_RS10250 - chemotaxis protein, at 2,142,478 to 2,143,029
_RS10250
AZOBR_RS10255: AZOBR_RS10255 - hypothetical protein, at 2,143,004 to 2,144,311
_RS10255
Position (kb)
2140
2141
2142
2143 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2139.649 kb on + strand, within AZOBR_RS10240 at 2139.760 kb on + strand, within AZOBR_RS10240 at 2139.760 kb on + strand, within AZOBR_RS10240 at 2139.987 kb on - strand, within AZOBR_RS10240 at 2140.289 kb on + strand, within AZOBR_RS10240 at 2140.316 kb on - strand, within AZOBR_RS10240 at 2140.532 kb on + strand at 2140.533 kb on - strand at 2140.714 kb on - strand at 2140.714 kb on - strand at 2140.747 kb on - strand at 2140.747 kb on - strand at 2141.052 kb on + strand, within AZOBR_RS10245 at 2141.083 kb on - strand, within AZOBR_RS10245 at 2141.490 kb on + strand, within AZOBR_RS10245 at 2141.490 kb on + strand, within AZOBR_RS10245 at 2141.491 kb on - strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.493 kb on + strand, within AZOBR_RS10245 at 2141.661 kb on + strand, within AZOBR_RS10245 at 2141.661 kb on + strand, within AZOBR_RS10245 at 2141.662 kb on - strand, within AZOBR_RS10245 at 2141.662 kb on - strand, within AZOBR_RS10245 at 2141.667 kb on + strand, within AZOBR_RS10245 at 2141.667 kb on + strand, within AZOBR_RS10245 at 2141.667 kb on + strand, within AZOBR_RS10245 at 2141.668 kb on - strand, within AZOBR_RS10245 at 2141.737 kb on - strand, within AZOBR_RS10245 at 2141.737 kb on - strand, within AZOBR_RS10245 at 2141.856 kb on + strand, within AZOBR_RS10245 at 2141.856 kb on + strand, within AZOBR_RS10245 at 2141.856 kb on + strand, within AZOBR_RS10245 at 2141.857 kb on - strand, within AZOBR_RS10245 at 2142.108 kb on + strand at 2142.359 kb on + strand at 2142.373 kb on + strand at 2142.373 kb on + strand at 2142.376 kb on + strand at 2142.380 kb on + strand at 2142.381 kb on - strand at 2142.406 kb on - strand at 2142.406 kb on - strand at 2142.500 kb on + strand at 2142.500 kb on + strand at 2142.500 kb on + strand at 2142.501 kb on - strand at 2142.501 kb on - strand at 2142.501 kb on - strand at 2142.501 kb on - strand at 2142.501 kb on - strand at 2142.586 kb on + strand, within AZOBR_RS10250 at 2142.688 kb on + strand, within AZOBR_RS10250 at 2142.689 kb on - strand, within AZOBR_RS10250 at 2142.689 kb on - strand, within AZOBR_RS10250 at 2142.736 kb on + strand, within AZOBR_RS10250 at 2142.736 kb on + strand, within AZOBR_RS10250 at 2142.737 kb on - strand, within AZOBR_RS10250 at 2142.769 kb on + strand, within AZOBR_RS10250 at 2142.841 kb on + strand, within AZOBR_RS10250 at 2142.842 kb on - strand, within AZOBR_RS10250 at 2142.898 kb on + strand, within AZOBR_RS10250 at 2142.899 kb on - strand, within AZOBR_RS10250 at 2142.899 kb on - strand, within AZOBR_RS10250 at 2143.004 kb on - strand at 2143.004 kb on - strand at 2143.027 kb on + strand at 2143.082 kb on + strand at 2143.083 kb on - strand at 2143.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days remove 2,139,649 + AZOBR_RS10240 0.21 +0.4 2,139,760 + AZOBR_RS10240 0.32 +0.5 2,139,760 + AZOBR_RS10240 0.32 -0.9 2,139,987 - AZOBR_RS10240 0.54 -1.0 2,140,289 + AZOBR_RS10240 0.84 -2.8 2,140,316 - AZOBR_RS10240 0.87 -3.5 2,140,532 + +0.5 2,140,533 - -0.7 2,140,714 - -0.5 2,140,714 - -0.4 2,140,747 - +0.1 2,140,747 - +0.2 2,141,052 + AZOBR_RS10245 0.29 +0.0 2,141,083 - AZOBR_RS10245 0.31 -0.1 2,141,490 + AZOBR_RS10245 0.56 +0.5 2,141,490 + AZOBR_RS10245 0.56 -1.4 2,141,491 - AZOBR_RS10245 0.56 -0.5 2,141,493 + AZOBR_RS10245 0.56 -0.7 2,141,493 + AZOBR_RS10245 0.56 +0.0 2,141,493 + AZOBR_RS10245 0.56 -0.1 2,141,493 + AZOBR_RS10245 0.56 +0.3 2,141,493 + AZOBR_RS10245 0.56 -0.0 2,141,493 + AZOBR_RS10245 0.56 -1.9 2,141,661 + AZOBR_RS10245 0.66 +0.2 2,141,661 + AZOBR_RS10245 0.66 -0.7 2,141,662 - AZOBR_RS10245 0.66 -2.4 2,141,662 - AZOBR_RS10245 0.66 +0.5 2,141,667 + AZOBR_RS10245 0.67 -0.5 2,141,667 + AZOBR_RS10245 0.67 +0.1 2,141,667 + AZOBR_RS10245 0.67 +0.4 2,141,668 - AZOBR_RS10245 0.67 +0.0 2,141,737 - AZOBR_RS10245 0.71 +0.4 2,141,737 - AZOBR_RS10245 0.71 +0.2 2,141,856 + AZOBR_RS10245 0.78 -1.0 2,141,856 + AZOBR_RS10245 0.78 -0.2 2,141,856 + AZOBR_RS10245 0.78 +0.1 2,141,857 - AZOBR_RS10245 0.78 -0.7 2,142,108 + -0.1 2,142,359 + -0.3 2,142,373 + +0.7 2,142,373 + +0.1 2,142,376 + -0.5 2,142,380 + +0.4 2,142,381 - +0.6 2,142,406 - -1.2 2,142,406 - -0.9 2,142,500 + +0.3 2,142,500 + +0.1 2,142,500 + +0.3 2,142,501 - +0.2 2,142,501 - -0.6 2,142,501 - +0.3 2,142,501 - -0.5 2,142,501 - -0.2 2,142,586 + AZOBR_RS10250 0.20 -0.3 2,142,688 + AZOBR_RS10250 0.38 +0.1 2,142,689 - AZOBR_RS10250 0.38 +0.4 2,142,689 - AZOBR_RS10250 0.38 +0.3 2,142,736 + AZOBR_RS10250 0.47 -2.1 2,142,736 + AZOBR_RS10250 0.47 +0.5 2,142,737 - AZOBR_RS10250 0.47 +0.2 2,142,769 + AZOBR_RS10250 0.53 -0.5 2,142,841 + AZOBR_RS10250 0.66 +0.1 2,142,842 - AZOBR_RS10250 0.66 -1.5 2,142,898 + AZOBR_RS10250 0.76 -0.9 2,142,899 - AZOBR_RS10250 0.76 +0.1 2,142,899 - AZOBR_RS10250 0.76 -1.5 2,143,004 - +0.8 2,143,004 - -1.1 2,143,027 + -3.6 2,143,082 + -0.7 2,143,083 - +1.4 2,143,083 - -1.7
Or see this region's nucleotide sequence