Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_622 and Pf1N1B4_623 are separated by 33 nucleotides Pf1N1B4_623 and Pf1N1B4_624 are separated by 379 nucleotides Pf1N1B4_624 and Pf1N1B4_625 are separated by 95 nucleotides
Pf1N1B4_622 - L-serine dehydratase (EC 4.3.1.17), at 720,585 to 721,961
_622
Pf1N1B4_623 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 721,995 to 723,119
_623
Pf1N1B4_624 - Cold shock protein CspA, at 723,499 to 723,711
_624
Pf1N1B4_625 - FIG023103: Predicted transmembrane protein, at 723,807 to 724,295
_625
Position (kb)
721
722
723
724 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 721.015 kb on - strand, within Pf1N1B4_622 at 721.017 kb on + strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.018 kb on - strand, within Pf1N1B4_622 at 721.143 kb on + strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.144 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622 at 721.396 kb on - strand, within Pf1N1B4_622 at 721.491 kb on + strand, within Pf1N1B4_622 at 721.491 kb on + strand, within Pf1N1B4_622 at 721.491 kb on + strand, within Pf1N1B4_622 at 721.491 kb on + strand, within Pf1N1B4_622 at 721.491 kb on + strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.492 kb on - strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.494 kb on + strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.495 kb on - strand, within Pf1N1B4_622 at 721.534 kb on + strand, within Pf1N1B4_622 at 721.535 kb on - strand, within Pf1N1B4_622 at 721.572 kb on + strand, within Pf1N1B4_622 at 721.572 kb on + strand, within Pf1N1B4_622 at 721.572 kb on + strand, within Pf1N1B4_622 at 721.573 kb on - strand at 721.573 kb on - strand, within Pf1N1B4_622 at 721.573 kb on - strand, within Pf1N1B4_622 at 721.573 kb on - strand, within Pf1N1B4_622 at 721.590 kb on - strand, within Pf1N1B4_622 at 721.590 kb on - strand, within Pf1N1B4_622 at 721.765 kb on + strand, within Pf1N1B4_622 at 721.765 kb on + strand, within Pf1N1B4_622 at 721.765 kb on + strand, within Pf1N1B4_622 at 721.765 kb on + strand, within Pf1N1B4_622 at 721.765 kb on + strand, within Pf1N1B4_622 at 721.766 kb on - strand, within Pf1N1B4_622 at 721.766 kb on - strand, within Pf1N1B4_622 at 721.766 kb on - strand, within Pf1N1B4_622 at 721.766 kb on - strand, within Pf1N1B4_622 at 721.766 kb on - strand, within Pf1N1B4_622 at 721.911 kb on + strand at 721.912 kb on - strand at 721.912 kb on - strand at 721.912 kb on - strand at 721.912 kb on - strand at 721.995 kb on - strand at 722.095 kb on - strand at 722.095 kb on - strand at 722.510 kb on + strand, within Pf1N1B4_623 at 722.510 kb on + strand, within Pf1N1B4_623 at 722.511 kb on - strand, within Pf1N1B4_623 at 722.892 kb on - strand, within Pf1N1B4_623 at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.076 kb on + strand at 723.105 kb on + strand at 723.105 kb on + strand at 723.106 kb on - strand at 723.108 kb on + strand at 723.131 kb on + strand at 723.131 kb on + strand at 723.132 kb on - strand at 723.132 kb on - strand at 723.132 kb on - strand at 723.133 kb on + strand at 723.134 kb on - strand at 723.134 kb on - strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.220 kb on - strand at 723.221 kb on + strand at 723.266 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.389 kb on - strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.396 kb on - strand at 723.399 kb on + strand at 723.399 kb on + strand at 723.400 kb on - strand at 723.400 kb on - strand at 723.421 kb on + strand at 723.422 kb on - strand at 723.445 kb on - strand at 723.695 kb on + strand at 723.695 kb on + strand at 723.695 kb on + strand at 723.737 kb on + strand at 723.766 kb on + strand at 723.766 kb on + strand at 723.767 kb on - strand at 723.775 kb on - strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.882 kb on + strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.896 kb on - strand, within Pf1N1B4_625 at 723.896 kb on - strand, within Pf1N1B4_625 at 723.930 kb on + strand, within Pf1N1B4_625 at 723.930 kb on + strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 724.016 kb on - strand, within Pf1N1B4_625
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 721,015 - Pf1N1B4_622 0.31 +0.2 721,017 + Pf1N1B4_622 0.31 -0.3 721,018 - Pf1N1B4_622 0.31 -1.4 721,018 - Pf1N1B4_622 0.31 -0.1 721,143 + Pf1N1B4_622 0.41 +1.6 721,144 - Pf1N1B4_622 0.41 +0.8 721,144 - Pf1N1B4_622 0.41 +1.8 721,144 - Pf1N1B4_622 0.41 -2.0 721,144 - Pf1N1B4_622 0.41 +1.3 721,396 - Pf1N1B4_622 0.59 +0.2 721,396 - Pf1N1B4_622 0.59 +1.3 721,491 + Pf1N1B4_622 0.66 -1.0 721,491 + Pf1N1B4_622 0.66 -2.0 721,491 + Pf1N1B4_622 0.66 -2.0 721,491 + Pf1N1B4_622 0.66 +0.7 721,491 + Pf1N1B4_622 0.66 +0.4 721,492 - Pf1N1B4_622 0.66 +2.1 721,492 - Pf1N1B4_622 0.66 -0.3 721,492 - Pf1N1B4_622 0.66 +1.1 721,492 - Pf1N1B4_622 0.66 -1.3 721,492 - Pf1N1B4_622 0.66 -1.0 721,492 - Pf1N1B4_622 0.66 +1.3 721,492 - Pf1N1B4_622 0.66 +1.8 721,492 - Pf1N1B4_622 0.66 -0.3 721,492 - Pf1N1B4_622 0.66 +1.6 721,494 + Pf1N1B4_622 0.66 +0.8 721,494 + Pf1N1B4_622 0.66 -0.4 721,494 + -1.0 721,494 + Pf1N1B4_622 0.66 -0.7 721,494 + Pf1N1B4_622 0.66 -0.0 721,494 + Pf1N1B4_622 0.66 -0.0 721,494 + Pf1N1B4_622 0.66 -0.4 721,494 + Pf1N1B4_622 0.66 +0.5 721,494 + Pf1N1B4_622 0.66 +1.8 721,494 + Pf1N1B4_622 0.66 -0.0 721,494 + Pf1N1B4_622 0.66 +2.6 721,494 + Pf1N1B4_622 0.66 +1.1 721,494 + Pf1N1B4_622 0.66 +1.6 721,495 - Pf1N1B4_622 0.66 -2.6 721,495 - -0.0 721,495 - Pf1N1B4_622 0.66 +0.1 721,495 - Pf1N1B4_622 0.66 -0.2 721,495 - Pf1N1B4_622 0.66 +1.3 721,495 - Pf1N1B4_622 0.66 -1.5 721,495 - Pf1N1B4_622 0.66 +2.0 721,495 - Pf1N1B4_622 0.66 -0.6 721,534 + Pf1N1B4_622 0.69 -0.0 721,535 - Pf1N1B4_622 0.69 -3.0 721,572 + Pf1N1B4_622 0.72 -0.0 721,572 + Pf1N1B4_622 0.72 +1.8 721,572 + Pf1N1B4_622 0.72 -1.0 721,573 - +1.3 721,573 - Pf1N1B4_622 0.72 +1.0 721,573 - Pf1N1B4_622 0.72 +0.3 721,573 - Pf1N1B4_622 0.72 -0.0 721,590 - Pf1N1B4_622 0.73 -0.0 721,590 - Pf1N1B4_622 0.73 -0.0 721,765 + Pf1N1B4_622 0.86 -0.0 721,765 + Pf1N1B4_622 0.86 -0.4 721,765 + Pf1N1B4_622 0.86 +0.7 721,765 + Pf1N1B4_622 0.86 -1.0 721,765 + Pf1N1B4_622 0.86 +0.6 721,766 - Pf1N1B4_622 0.86 -0.0 721,766 - Pf1N1B4_622 0.86 -1.0 721,766 - Pf1N1B4_622 0.86 +0.6 721,766 - Pf1N1B4_622 0.86 +1.0 721,766 - Pf1N1B4_622 0.86 -0.6 721,911 + +1.6 721,912 - +0.8 721,912 - -0.0 721,912 - +1.0 721,912 - +0.6 721,995 - +0.9 722,095 - -1.0 722,095 - +0.8 722,510 + Pf1N1B4_623 0.46 +2.1 722,510 + Pf1N1B4_623 0.46 -0.0 722,511 - Pf1N1B4_623 0.46 +0.8 722,892 - Pf1N1B4_623 0.80 +0.8 723,015 + +2.3 723,015 + -0.0 723,015 + +1.0 723,015 + -1.0 723,015 + +0.6 723,015 + +1.3 723,015 + -0.0 723,015 + -0.3 723,015 + -0.3 723,015 + +0.3 723,016 - +0.1 723,016 - +0.6 723,016 - -0.0 723,016 - -1.0 723,016 - +2.0 723,016 - +1.0 723,076 + -0.0 723,105 + +0.7 723,105 + +0.4 723,106 - +1.8 723,108 + +1.3 723,131 + -2.6 723,131 + +2.4 723,132 - +0.8 723,132 - -0.0 723,132 - -0.3 723,133 + +1.3 723,134 - +0.8 723,134 - -0.0 723,219 + -0.0 723,219 + -1.0 723,219 + -0.0 723,219 + +1.8 723,220 - +1.9 723,221 + -0.0 723,266 + +1.3 723,388 + +2.0 723,388 + -0.4 723,388 + -0.0 723,388 + -2.0 723,388 + +1.3 723,388 + -1.0 723,389 - +0.6 723,395 + -2.0 723,395 + +1.6 723,395 + -0.2 723,395 + +2.4 723,395 + -1.0 723,396 - +0.8 723,399 + +1.1 723,399 + -1.0 723,400 - +2.8 723,400 - -1.0 723,421 + +1.3 723,422 - +2.0 723,445 - -1.0 723,695 + -0.4 723,695 + -0.0 723,695 + +0.8 723,737 + +2.0 723,766 + +0.6 723,766 + +1.0 723,767 - +0.6 723,775 - +2.1 723,823 + +2.1 723,823 + -0.3 723,823 + -0.4 723,823 + +0.2 723,823 + -0.1 723,823 + +1.3 723,823 + -0.0 723,823 + +0.1 723,823 + +0.6 723,823 + +1.3 723,823 + -0.0 723,823 + -1.0 723,824 - +1.0 723,824 - -0.4 723,824 - +0.7 723,824 - -1.0 723,824 - +1.0 723,824 - -1.0 723,824 - -2.0 723,824 - +0.8 723,824 - +1.6 723,824 - -0.3 723,882 + Pf1N1B4_625 0.15 -0.1 723,883 - Pf1N1B4_625 0.16 +0.6 723,883 - Pf1N1B4_625 0.16 -0.6 723,883 - Pf1N1B4_625 0.16 -0.0 723,896 - Pf1N1B4_625 0.18 -3.0 723,896 - Pf1N1B4_625 0.18 +0.6 723,930 + Pf1N1B4_625 0.25 +2.3 723,930 + Pf1N1B4_625 0.25 +1.3 723,931 - Pf1N1B4_625 0.25 -2.0 723,931 - Pf1N1B4_625 0.25 -0.7 723,931 - Pf1N1B4_625 0.25 +0.4 724,016 - Pf1N1B4_625 0.43 -0.0
Or see this region's nucleotide sequence