Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_60 and Pf1N1B4_61 are separated by 276 nucleotides Pf1N1B4_61 and Pf1N1B4_62 are separated by 71 nucleotides Pf1N1B4_62 and Pf1N1B4_63 are separated by 200 nucleotides Pf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotides
Pf1N1B4_60 - Serine phosphatase RsbU, regulator of sigma subunit, at 64,018 to 65,199
_60
Pf1N1B4_61 - FIG00953170: hypothetical protein, at 65,476 to 65,775
_61
Pf1N1B4_62 - VacJ-like lipoprotein precursor, at 65,847 to 66,536
_62
Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357
_63
Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730
_64
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 65.279 kb on - strand at 65.315 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.325 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.434 kb on + strand at 65.434 kb on + strand at 65.434 kb on + strand at 65.497 kb on + strand at 65.498 kb on - strand at 65.754 kb on + strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.773 kb on + strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.600 kb on - strand at 66.600 kb on - strand at 66.619 kb on - strand at 66.707 kb on + strand at 66.716 kb on + strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.737 kb on - strand at 66.786 kb on + strand at 66.786 kb on + strand at 66.787 kb on - strand at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.991 kb on + strand at 66.992 kb on - strand at 66.992 kb on - strand, within Pf1N1B4_63 at 67.322 kb on - strand at 67.390 kb on - strand at 67.394 kb on + strand at 67.394 kb on + strand at 67.395 kb on - strand at 67.395 kb on - strand at 67.408 kb on - strand at 67.408 kb on - strand at 67.433 kb on + strand at 67.434 kb on - strand at 67.451 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 65,279 - -0.3 65,315 - -0.3 65,319 - +1.0 65,319 - -1.3 65,319 - -1.3 65,319 - -0.3 65,319 - +0.3 65,319 - +0.9 65,319 - -0.3 65,319 - +1.3 65,325 - +1.1 65,344 - +0.3 65,344 - -1.3 65,410 + -0.7 65,410 + +1.3 65,410 + +2.9 65,410 + -2.3 65,410 + +1.5 65,410 + -1.7 65,410 + -0.3 65,410 + +0.7 65,410 + +1.0 65,410 + -0.3 65,410 + +0.7 65,410 + -0.3 65,434 + -0.3 65,434 + -0.5 65,434 + -0.5 65,497 + +0.7 65,498 - +0.7 65,754 + -2.9 65,755 - -0.1 65,755 - +1.3 65,755 - +1.3 65,755 - +0.0 65,755 - -0.3 65,773 + +0.0 66,598 - -1.8 66,598 - +2.8 66,598 - +2.7 66,598 - -0.1 66,598 - +0.3 66,598 - +0.7 66,600 - -1.3 66,600 - -1.3 66,619 - -2.3 66,707 + +1.9 66,716 + +1.9 66,717 - +1.0 66,717 - -0.5 66,717 - -0.7 66,717 - -1.3 66,719 + +1.5 66,720 - -0.8 66,720 - +1.7 66,720 - -0.3 66,720 - -0.9 66,720 - +0.6 66,737 - +0.7 66,786 + -0.1 66,786 + -0.1 66,787 - +1.3 66,830 - Pf1N1B4_63 0.15 -1.3 66,830 - Pf1N1B4_63 0.15 +0.0 66,830 - Pf1N1B4_63 0.15 -0.1 66,991 + -0.3 66,992 - +0.3 66,992 - Pf1N1B4_63 0.41 -0.7 67,322 - -0.5 67,390 - -2.0 67,394 + +0.7 67,394 + +2.2 67,395 - +0.3 67,395 - +2.0 67,408 - +0.3 67,408 - -0.7 67,433 + -0.3 67,434 - -0.3 67,451 + +0.3
Or see this region's nucleotide sequence