Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5958 and Pf1N1B4_5959 are separated by 311 nucleotides Pf1N1B4_5959 and Pf1N1B4_5960 are separated by 7 nucleotides Pf1N1B4_5960 and Pf1N1B4_5961 are separated by 63 nucleotides
Pf1N1B4_5958 - Ferrichrome-iron receptor, at 406,249 to 408,372
_5958
Pf1N1B4_5959 - Probable signal peptide protein, at 408,684 to 408,947
_5959
Pf1N1B4_5960 - Cytochrome b, at 408,955 to 409,482
_5960
Pf1N1B4_5961 - Major facilitator superfamily transporter, at 409,546 to 411,222
_5961
Position (kb)
408
409 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 407.698 kb on + strand, within Pf1N1B4_5958 at 407.699 kb on - strand, within Pf1N1B4_5958 at 407.701 kb on + strand, within Pf1N1B4_5958 at 407.701 kb on + strand, within Pf1N1B4_5958 at 407.702 kb on - strand, within Pf1N1B4_5958 at 407.723 kb on - strand, within Pf1N1B4_5958 at 407.758 kb on - strand, within Pf1N1B4_5958 at 407.758 kb on - strand, within Pf1N1B4_5958 at 407.986 kb on + strand at 407.986 kb on + strand, within Pf1N1B4_5958 at 408.001 kb on + strand, within Pf1N1B4_5958 at 408.001 kb on + strand, within Pf1N1B4_5958 at 408.151 kb on + strand, within Pf1N1B4_5958 at 408.151 kb on + strand, within Pf1N1B4_5958 at 408.151 kb on + strand, within Pf1N1B4_5958 at 408.151 kb on + strand, within Pf1N1B4_5958 at 408.152 kb on - strand, within Pf1N1B4_5958 at 408.152 kb on - strand at 408.152 kb on - strand, within Pf1N1B4_5958 at 408.152 kb on - strand, within Pf1N1B4_5958 at 408.152 kb on - strand, within Pf1N1B4_5958 at 408.152 kb on - strand at 408.152 kb on - strand, within Pf1N1B4_5958 at 408.202 kb on + strand at 408.202 kb on + strand at 408.202 kb on + strand at 408.202 kb on + strand at 408.286 kb on + strand at 408.286 kb on + strand at 408.287 kb on - strand at 408.287 kb on - strand at 408.287 kb on - strand at 408.289 kb on + strand at 408.289 kb on + strand at 408.289 kb on + strand at 408.361 kb on + strand at 408.411 kb on + strand at 408.411 kb on + strand at 408.412 kb on - strand at 408.412 kb on - strand at 408.528 kb on + strand at 408.529 kb on - strand at 408.537 kb on - strand at 408.542 kb on + strand at 408.542 kb on + strand at 408.543 kb on - strand at 408.667 kb on + strand at 408.667 kb on + strand at 408.667 kb on + strand at 408.667 kb on + strand at 408.668 kb on - strand at 408.668 kb on - strand at 408.690 kb on - strand at 408.817 kb on - strand, within Pf1N1B4_5959 at 408.855 kb on + strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.901 kb on - strand at 408.901 kb on - strand, within Pf1N1B4_5959 at 408.945 kb on + strand at 408.992 kb on + strand at 408.992 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 409.084 kb on + strand, within Pf1N1B4_5960 at 409.085 kb on - strand, within Pf1N1B4_5960 at 409.091 kb on + strand, within Pf1N1B4_5960 at 409.092 kb on - strand, within Pf1N1B4_5960 at 409.321 kb on + strand, within Pf1N1B4_5960 at 409.321 kb on + strand, within Pf1N1B4_5960 at 409.322 kb on - strand, within Pf1N1B4_5960 at 409.322 kb on - strand, within Pf1N1B4_5960 at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.646 kb on + strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.797 kb on - strand, within Pf1N1B4_5961 at 409.797 kb on - strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 407,698 + Pf1N1B4_5958 0.68 +0.9 407,699 - Pf1N1B4_5958 0.68 -1.5 407,701 + Pf1N1B4_5958 0.68 -1.5 407,701 + Pf1N1B4_5958 0.68 +0.7 407,702 - Pf1N1B4_5958 0.68 +0.9 407,723 - Pf1N1B4_5958 0.69 +0.7 407,758 - Pf1N1B4_5958 0.71 +0.5 407,758 - Pf1N1B4_5958 0.71 -1.5 407,986 + -0.5 407,986 + Pf1N1B4_5958 0.82 +0.1 408,001 + Pf1N1B4_5958 0.82 -0.2 408,001 + Pf1N1B4_5958 0.82 +0.9 408,151 + Pf1N1B4_5958 0.90 -0.9 408,151 + Pf1N1B4_5958 0.90 +0.5 408,151 + Pf1N1B4_5958 0.90 -1.5 408,151 + Pf1N1B4_5958 0.90 -0.2 408,152 - Pf1N1B4_5958 0.90 -1.5 408,152 - +0.1 408,152 - Pf1N1B4_5958 0.90 +1.5 408,152 - Pf1N1B4_5958 0.90 +0.4 408,152 - Pf1N1B4_5958 0.90 +1.1 408,152 - -3.5 408,152 - Pf1N1B4_5958 0.90 -2.0 408,202 + -1.5 408,202 + -0.1 408,202 + +0.1 408,202 + -0.6 408,286 + +0.9 408,286 + -2.5 408,287 - +1.1 408,287 - +1.6 408,287 - -1.1 408,289 + +2.0 408,289 + +0.5 408,289 + -2.0 408,361 + +1.0 408,411 + -0.2 408,411 + +1.5 408,412 - +0.5 408,412 - +0.1 408,528 + -1.3 408,529 - -0.2 408,537 - -1.5 408,542 + -0.5 408,542 + +0.5 408,543 - +0.1 408,667 + -1.5 408,667 + +0.8 408,667 + +1.4 408,667 + -1.5 408,668 - +0.9 408,668 - +0.4 408,690 - +0.9 408,817 - Pf1N1B4_5959 0.50 -0.9 408,855 + Pf1N1B4_5959 0.65 -1.5 408,865 - Pf1N1B4_5959 0.69 +0.0 408,865 - Pf1N1B4_5959 0.69 +0.5 408,865 - Pf1N1B4_5959 0.69 -0.2 408,900 + Pf1N1B4_5959 0.82 -0.7 408,900 + Pf1N1B4_5959 0.82 -0.5 408,900 + Pf1N1B4_5959 0.82 -0.4 408,901 - -0.5 408,901 - Pf1N1B4_5959 0.82 -1.5 408,945 + +1.1 408,992 + -0.1 408,992 + -0.9 408,996 + -1.5 408,996 + +0.1 408,996 + -0.1 408,996 + -0.5 408,996 + +0.9 408,996 + +0.7 408,996 + +1.0 409,084 + Pf1N1B4_5960 0.24 +0.1 409,085 - Pf1N1B4_5960 0.25 -0.1 409,091 + Pf1N1B4_5960 0.26 +0.5 409,092 - Pf1N1B4_5960 0.26 +0.0 409,321 + Pf1N1B4_5960 0.69 +2.0 409,321 + Pf1N1B4_5960 0.69 +1.9 409,322 - Pf1N1B4_5960 0.70 -0.5 409,322 - Pf1N1B4_5960 0.70 +0.4 409,547 + +0.1 409,547 + +0.3 409,547 + +1.2 409,547 + -0.2 409,547 + -0.9 409,547 + +0.5 409,548 - +1.1 409,548 - +1.4 409,548 - -1.2 409,548 - -0.1 409,548 - -1.3 409,548 - +0.2 409,548 - +0.1 409,548 - -1.1 409,548 - +0.7 409,548 - -0.9 409,646 + +0.1 409,647 - -1.5 409,647 - +1.1 409,647 - +1.4 409,647 - +1.2 409,647 - -0.5 409,647 - -1.0 409,797 - Pf1N1B4_5961 0.15 +0.5 409,797 - Pf1N1B4_5961 0.15 -0.7 409,811 + Pf1N1B4_5961 0.16 +1.4 409,811 + Pf1N1B4_5961 0.16 +0.1 409,811 + Pf1N1B4_5961 0.16 +0.1 409,812 - Pf1N1B4_5961 0.16 +1.2 409,812 - Pf1N1B4_5961 0.16 +0.5 409,812 - Pf1N1B4_5961 0.16 -1.0 409,812 - Pf1N1B4_5961 0.16 -0.5 409,812 - Pf1N1B4_5961 0.16 -0.1 409,812 - Pf1N1B4_5961 0.16 -0.7 409,812 - Pf1N1B4_5961 0.16 -0.9
Or see this region's nucleotide sequence