Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5924 and Pf1N1B4_5925 are separated by 53 nucleotides Pf1N1B4_5925 and Pf1N1B4_5926 are separated by 38 nucleotides
Pf1N1B4_5924 - hypothetical protein, at 369,220 to 369,531
_5924
Pf1N1B4_5925 - hypothetical protein, at 369,585 to 370,298
_5925
Pf1N1B4_5926 - hypothetical protein, at 370,337 to 371,005
_5926
Position (kb) 369
370
371 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 368.586 kb on - strand at 368.586 kb on - strand at 368.586 kb on - strand at 368.586 kb on - strand at 368.595 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.622 kb on - strand at 368.649 kb on + strand at 368.650 kb on - strand at 368.650 kb on - strand at 368.650 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.668 kb on - strand at 368.691 kb on - strand at 368.691 kb on - strand at 368.731 kb on - strand at 368.733 kb on - strand at 368.766 kb on - strand at 368.766 kb on - strand at 368.786 kb on + strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.787 kb on - strand at 368.792 kb on + strand at 368.792 kb on + strand at 368.793 kb on - strand at 368.793 kb on - strand at 368.808 kb on + strand at 368.842 kb on - strand at 368.891 kb on + strand at 368.891 kb on + strand at 368.891 kb on + strand at 368.892 kb on - strand at 368.892 kb on - strand at 368.897 kb on + strand at 368.901 kb on + strand at 368.917 kb on + strand at 368.919 kb on + strand at 368.919 kb on + strand at 368.920 kb on - strand at 368.920 kb on - strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.924 kb on + strand at 368.925 kb on - strand at 368.925 kb on - strand at 368.925 kb on - strand at 369.005 kb on + strand at 369.005 kb on + strand at 369.005 kb on + strand at 369.006 kb on - strand at 369.006 kb on - strand at 369.009 kb on + strand at 369.010 kb on - strand at 369.039 kb on - strand at 369.039 kb on - strand at 369.042 kb on + strand at 369.067 kb on - strand at 369.082 kb on + strand at 369.082 kb on + strand at 369.112 kb on + strand at 369.112 kb on + strand at 369.112 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.143 kb on + strand at 369.144 kb on - strand at 369.144 kb on - strand at 369.147 kb on + strand at 369.214 kb on - strand at 369.215 kb on + strand at 369.215 kb on + strand at 369.216 kb on - strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.248 kb on + strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.249 kb on - strand at 369.250 kb on + strand at 369.250 kb on + strand at 369.250 kb on + strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.251 kb on - strand at 369.328 kb on - strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.369 kb on + strand, within Pf1N1B4_5924 at 369.370 kb on - strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.371 kb on + strand, within Pf1N1B4_5924 at 369.372 kb on - strand, within Pf1N1B4_5924 at 369.379 kb on + strand, within Pf1N1B4_5924 at 369.407 kb on + strand, within Pf1N1B4_5924 at 369.423 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.464 kb on + strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.465 kb on - strand, within Pf1N1B4_5924 at 369.529 kb on + strand at 369.529 kb on + strand at 369.529 kb on + strand at 369.529 kb on + strand at 369.530 kb on - strand at 369.563 kb on + strand at 369.563 kb on + strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.564 kb on - strand at 369.590 kb on - strand at 369.645 kb on + strand at 369.646 kb on - strand at 369.723 kb on + strand, within Pf1N1B4_5925 at 369.723 kb on + strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand, within Pf1N1B4_5925 at 369.724 kb on - strand at 369.726 kb on + strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.727 kb on - strand, within Pf1N1B4_5925 at 369.908 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.921 kb on - strand, within Pf1N1B4_5925 at 369.966 kb on - strand, within Pf1N1B4_5925 at 369.978 kb on - strand, within Pf1N1B4_5925 at 369.986 kb on - strand, within Pf1N1B4_5925 at 370.024 kb on - strand, within Pf1N1B4_5925 at 370.030 kb on + strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.031 kb on - strand, within Pf1N1B4_5925 at 370.078 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.082 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.153 kb on - strand, within Pf1N1B4_5925 at 370.272 kb on + strand at 370.272 kb on + strand at 370.273 kb on - strand at 370.273 kb on - strand at 370.273 kb on - strand at 370.275 kb on + strand at 370.276 kb on - strand at 370.276 kb on - strand at 370.276 kb on - strand at 370.292 kb on + strand at 370.293 kb on - strand at 370.321 kb on - strand at 370.321 kb on - strand at 370.333 kb on + strand at 370.334 kb on - strand at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.424 kb on + strand, within Pf1N1B4_5926 at 370.425 kb on - strand, within Pf1N1B4_5926 at 370.428 kb on + strand, within Pf1N1B4_5926 at 370.428 kb on + strand, within Pf1N1B4_5926 at 370.429 kb on - strand, within Pf1N1B4_5926 at 370.429 kb on - strand, within Pf1N1B4_5926 at 370.515 kb on + strand, within Pf1N1B4_5926 at 370.516 kb on - strand, within Pf1N1B4_5926 at 370.567 kb on + strand, within Pf1N1B4_5926 at 370.575 kb on + strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.576 kb on - strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.711 kb on + strand, within Pf1N1B4_5926 at 370.712 kb on - strand, within Pf1N1B4_5926 at 370.712 kb on - strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.762 kb on + strand, within Pf1N1B4_5926 at 370.763 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.825 kb on - strand, within Pf1N1B4_5926 at 370.843 kb on + strand, within Pf1N1B4_5926 at 370.843 kb on + strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.844 kb on - strand, within Pf1N1B4_5926 at 370.848 kb on + strand, within Pf1N1B4_5926 at 370.875 kb on - strand, within Pf1N1B4_5926 at 370.875 kb on - strand, within Pf1N1B4_5926 at 370.876 kb on + strand, within Pf1N1B4_5926 at 370.876 kb on + strand, within Pf1N1B4_5926 at 370.877 kb on - strand, within Pf1N1B4_5926 at 370.877 kb on - strand, within Pf1N1B4_5926 at 370.989 kb on + strand at 370.989 kb on + strand at 370.989 kb on + strand at 370.990 kb on - strand at 370.990 kb on - strand at 371.059 kb on - strand at 371.125 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.247 kb on - strand at 371.263 kb on + strand at 371.290 kb on - strand at 371.296 kb on + strand at 371.296 kb on + strand at 371.296 kb on + strand at 371.297 kb on - strand at 371.297 kb on - strand at 371.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 368,586 - +0.7 368,586 - +1.3 368,586 - +0.0 368,586 - +0.8 368,595 - +0.1 368,622 - +0.1 368,622 - -0.1 368,622 - +0.8 368,622 - -0.3 368,622 - +0.1 368,649 + -0.0 368,650 - +1.0 368,650 - -0.2 368,650 - +0.9 368,668 - -0.9 368,668 - -0.7 368,668 - -0.7 368,668 - +0.2 368,691 - +0.0 368,691 - -0.8 368,731 - -0.5 368,733 - -1.0 368,766 - -0.8 368,766 - -1.0 368,786 + +0.5 368,787 - -2.8 368,787 - +0.1 368,787 - -0.2 368,787 - -0.6 368,787 - +1.4 368,787 - -0.7 368,792 + +1.1 368,792 + -0.9 368,793 - +0.2 368,793 - -0.9 368,808 + -0.7 368,842 - +0.0 368,891 + +0.7 368,891 + +1.5 368,891 + +1.4 368,892 - -0.2 368,892 - +0.8 368,897 + +1.2 368,901 + +0.6 368,917 + +1.2 368,919 + -0.1 368,919 + +0.5 368,920 - -1.2 368,920 - -0.6 368,924 + +1.5 368,924 + -3.8 368,924 + +2.0 368,924 + -0.4 368,925 - -0.6 368,925 - -0.0 368,925 - -1.5 369,005 + -1.5 369,005 + -0.9 369,005 + -1.5 369,006 - +0.5 369,006 - -1.1 369,009 + +0.4 369,010 - -0.5 369,039 - +0.5 369,039 - -2.0 369,042 + +0.8 369,067 - +0.5 369,082 + +1.8 369,082 + +1.5 369,112 + +0.8 369,112 + -1.8 369,112 + +0.5 369,143 + +2.1 369,143 + -0.5 369,143 + +0.5 369,143 + +0.6 369,143 + -0.1 369,143 + +0.9 369,144 - -1.1 369,144 - -0.0 369,147 + +1.4 369,214 - +0.5 369,215 + +0.5 369,215 + +0.4 369,216 - +1.5 369,248 + +0.7 369,248 + -0.5 369,248 + +0.6 369,248 + +1.0 369,249 - +2.4 369,249 - -0.7 369,249 - -1.5 369,249 - +0.0 369,249 - +1.1 369,250 + -0.1 369,250 + -2.0 369,250 + +0.6 369,251 - -0.6 369,251 - -1.3 369,251 - -2.2 369,251 - +1.0 369,328 - Pf1N1B4_5924 0.35 -0.5 369,369 + Pf1N1B4_5924 0.48 -0.2 369,369 + Pf1N1B4_5924 0.48 +2.3 369,369 + Pf1N1B4_5924 0.48 +0.8 369,370 - Pf1N1B4_5924 0.48 -0.1 369,371 + Pf1N1B4_5924 0.48 +0.0 369,371 + Pf1N1B4_5924 0.48 +0.5 369,371 + Pf1N1B4_5924 0.48 +1.7 369,371 + Pf1N1B4_5924 0.48 +0.1 369,372 - Pf1N1B4_5924 0.49 -0.1 369,379 + Pf1N1B4_5924 0.51 +0.4 369,407 + Pf1N1B4_5924 0.60 +0.2 369,423 + Pf1N1B4_5924 0.65 +0.7 369,464 + Pf1N1B4_5924 0.78 -0.4 369,464 + Pf1N1B4_5924 0.78 -0.1 369,464 + Pf1N1B4_5924 0.78 +0.7 369,465 - Pf1N1B4_5924 0.79 -0.2 369,465 - Pf1N1B4_5924 0.79 +0.4 369,465 - Pf1N1B4_5924 0.79 +0.1 369,465 - Pf1N1B4_5924 0.79 -1.1 369,529 + -0.5 369,529 + +0.9 369,529 + +1.4 369,529 + +1.0 369,530 - -0.8 369,563 + +1.2 369,563 + -1.5 369,564 - +1.0 369,564 - +0.1 369,564 - -1.0 369,564 - -0.2 369,564 - +0.4 369,564 - +0.1 369,564 - -1.1 369,564 - -0.5 369,564 - +0.0 369,590 - -0.0 369,645 + +2.0 369,646 - -0.1 369,723 + Pf1N1B4_5925 0.19 -0.2 369,723 + Pf1N1B4_5925 0.19 +0.5 369,724 - Pf1N1B4_5925 0.19 -0.3 369,724 - Pf1N1B4_5925 0.19 +1.0 369,724 - Pf1N1B4_5925 0.19 -0.0 369,724 - Pf1N1B4_5925 0.19 +0.4 369,724 - Pf1N1B4_5925 0.19 +0.2 369,724 - Pf1N1B4_5925 0.19 +0.1 369,724 - Pf1N1B4_5925 0.19 +0.4 369,724 - -0.5 369,726 + Pf1N1B4_5925 0.20 -0.5 369,727 - Pf1N1B4_5925 0.20 -1.1 369,727 - Pf1N1B4_5925 0.20 -1.5 369,727 - Pf1N1B4_5925 0.20 -0.1 369,908 - Pf1N1B4_5925 0.45 -0.2 369,921 - Pf1N1B4_5925 0.47 -0.6 369,921 - Pf1N1B4_5925 0.47 +0.7 369,921 - Pf1N1B4_5925 0.47 -0.5 369,921 - Pf1N1B4_5925 0.47 +0.5 369,966 - Pf1N1B4_5925 0.53 +1.4 369,978 - Pf1N1B4_5925 0.55 -0.8 369,986 - Pf1N1B4_5925 0.56 -0.7 370,024 - Pf1N1B4_5925 0.61 -1.6 370,030 + Pf1N1B4_5925 0.62 -1.0 370,031 - Pf1N1B4_5925 0.62 -0.8 370,031 - Pf1N1B4_5925 0.62 -0.9 370,031 - Pf1N1B4_5925 0.62 -0.5 370,031 - Pf1N1B4_5925 0.62 -0.0 370,078 - Pf1N1B4_5925 0.69 -2.8 370,082 - -1.5 370,082 - Pf1N1B4_5925 0.70 +2.2 370,082 - Pf1N1B4_5925 0.70 +0.1 370,082 - Pf1N1B4_5925 0.70 -1.5 370,082 - Pf1N1B4_5925 0.70 -0.1 370,082 - Pf1N1B4_5925 0.70 +1.0 370,082 - Pf1N1B4_5925 0.70 +0.7 370,082 - Pf1N1B4_5925 0.70 -0.5 370,153 - Pf1N1B4_5925 0.80 +0.1 370,153 - Pf1N1B4_5925 0.80 -0.8 370,153 - Pf1N1B4_5925 0.80 -1.1 370,153 - Pf1N1B4_5925 0.80 -2.5 370,153 - Pf1N1B4_5925 0.80 -0.7 370,272 + +1.4 370,272 + +0.1 370,273 - -0.2 370,273 - -0.6 370,273 - -1.5 370,275 + -0.0 370,276 - -0.3 370,276 - -1.5 370,276 - +0.9 370,292 + +0.7 370,293 - +0.5 370,321 - -0.7 370,321 - -1.8 370,333 + -2.0 370,334 - -1.5 370,424 + Pf1N1B4_5926 0.13 -0.8 370,424 + Pf1N1B4_5926 0.13 -0.1 370,424 + Pf1N1B4_5926 0.13 -0.3 370,424 + Pf1N1B4_5926 0.13 -1.0 370,424 + Pf1N1B4_5926 0.13 -0.1 370,425 - Pf1N1B4_5926 0.13 -0.6 370,428 + Pf1N1B4_5926 0.14 -0.9 370,428 + Pf1N1B4_5926 0.14 -0.2 370,429 - Pf1N1B4_5926 0.14 -0.7 370,429 - Pf1N1B4_5926 0.14 -1.0 370,515 + Pf1N1B4_5926 0.27 -0.5 370,516 - Pf1N1B4_5926 0.27 -1.1 370,567 + Pf1N1B4_5926 0.34 -0.2 370,575 + Pf1N1B4_5926 0.36 -0.7 370,576 - Pf1N1B4_5926 0.36 -2.5 370,576 - Pf1N1B4_5926 0.36 -0.6 370,576 - Pf1N1B4_5926 0.36 -0.0 370,576 - Pf1N1B4_5926 0.36 -0.0 370,711 + Pf1N1B4_5926 0.56 +0.5 370,711 + Pf1N1B4_5926 0.56 -0.3 370,711 + Pf1N1B4_5926 0.56 -0.2 370,712 - Pf1N1B4_5926 0.56 -0.7 370,712 - Pf1N1B4_5926 0.56 -1.2 370,762 + Pf1N1B4_5926 0.64 -0.6 370,762 + Pf1N1B4_5926 0.64 -0.1 370,762 + Pf1N1B4_5926 0.64 +0.2 370,763 - Pf1N1B4_5926 0.64 +1.7 370,825 - Pf1N1B4_5926 0.73 -0.3 370,825 - Pf1N1B4_5926 0.73 -0.3 370,825 - Pf1N1B4_5926 0.73 -1.6 370,843 + Pf1N1B4_5926 0.76 -2.5 370,843 + Pf1N1B4_5926 0.76 -0.1 370,844 - Pf1N1B4_5926 0.76 -0.3 370,844 - Pf1N1B4_5926 0.76 +0.7 370,844 - Pf1N1B4_5926 0.76 -1.5 370,848 + Pf1N1B4_5926 0.76 -0.1 370,875 - Pf1N1B4_5926 0.80 +0.1 370,875 - Pf1N1B4_5926 0.80 -0.9 370,876 + Pf1N1B4_5926 0.81 -0.1 370,876 + Pf1N1B4_5926 0.81 -0.5 370,877 - Pf1N1B4_5926 0.81 -1.5 370,877 - Pf1N1B4_5926 0.81 -0.6 370,989 + +2.2 370,989 + -2.0 370,989 + +0.7 370,990 - +0.4 370,990 - +0.9 371,059 - +2.4 371,125 - -1.0 371,247 - +0.1 371,247 - +1.4 371,247 - +0.4 371,247 - +0.2 371,263 + -1.0 371,290 - -0.7 371,296 + +0.8 371,296 + +0.3 371,296 + -0.3 371,297 - +1.1 371,297 - +1.5 371,297 - -1.9
Or see this region's nucleotide sequence