Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5546 and Pf1N1B4_5547 are separated by 37 nucleotides Pf1N1B4_5547 and Pf1N1B4_5548 are separated by 150 nucleotides Pf1N1B4_5548 and Pf1N1B4_5549 are separated by 989 nucleotides
Pf1N1B4_5546 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 317,519 to 318,460
_5546
Pf1N1B4_5547 - hypothetical protein, at 318,498 to 318,779
_5547
Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943
_5548
Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061
_5549
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 317.940 kb on + strand, within Pf1N1B4_5546 at 317.948 kb on + strand, within Pf1N1B4_5546 at 317.948 kb on + strand, within Pf1N1B4_5546 at 317.985 kb on + strand, within Pf1N1B4_5546 at 317.998 kb on + strand, within Pf1N1B4_5546 at 317.998 kb on + strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 317.999 kb on - strand, within Pf1N1B4_5546 at 318.105 kb on + strand, within Pf1N1B4_5546 at 318.130 kb on + strand, within Pf1N1B4_5546 at 318.131 kb on - strand, within Pf1N1B4_5546 at 318.167 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.249 kb on + strand, within Pf1N1B4_5546 at 318.250 kb on - strand, within Pf1N1B4_5546 at 318.253 kb on + strand at 318.253 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.315 kb on + strand, within Pf1N1B4_5546 at 318.316 kb on - strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.358 kb on + strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.359 kb on - strand, within Pf1N1B4_5546 at 318.432 kb on + strand at 318.440 kb on + strand at 318.610 kb on - strand, within Pf1N1B4_5547 at 318.651 kb on - strand, within Pf1N1B4_5547 at 318.896 kb on + strand at 318.897 kb on - strand at 318.920 kb on + strand at 318.986 kb on - strand at 318.986 kb on - strand at 318.986 kb on - strand at 318.986 kb on - strand at 319.027 kb on + strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.028 kb on - strand at 319.044 kb on + strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.060 kb on - strand, within Pf1N1B4_5548 at 319.182 kb on + strand, within Pf1N1B4_5548 at 319.360 kb on - strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.408 kb on + strand, within Pf1N1B4_5548 at 319.502 kb on + strand, within Pf1N1B4_5548 at 319.503 kb on - strand, within Pf1N1B4_5548 at 319.503 kb on - strand, within Pf1N1B4_5548 at 319.596 kb on + strand, within Pf1N1B4_5548 at 319.611 kb on + strand, within Pf1N1B4_5548 at 319.611 kb on + strand, within Pf1N1B4_5548 at 319.612 kb on - strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.627 kb on + strand, within Pf1N1B4_5548 at 319.628 kb on - strand, within Pf1N1B4_5548 at 319.716 kb on + strand, within Pf1N1B4_5548 at 319.730 kb on - strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.779 kb on + strand, within Pf1N1B4_5548 at 319.780 kb on - strand, within Pf1N1B4_5548 at 319.858 kb on + strand at 319.858 kb on + strand at 319.859 kb on - strand at 319.925 kb on - strand at 319.925 kb on - strand at 319.925 kb on - strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.926 kb on + strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.927 kb on - strand at 319.947 kb on + strand at 319.948 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.966 kb on + strand at 319.971 kb on + strand at 319.975 kb on - strand at 319.975 kb on - strand at 320.021 kb on + strand at 320.022 kb on - strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.174 kb on + strand at 320.174 kb on + strand at 320.175 kb on - strand at 320.175 kb on - strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.179 kb on - strand at 320.179 kb on - strand at 320.200 kb on - strand at 320.255 kb on + strand at 320.256 kb on - strand at 320.277 kb on - strand at 320.282 kb on - strand at 320.339 kb on + strand at 320.339 kb on + strand at 320.340 kb on - strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.367 kb on + strand at 320.367 kb on + strand at 320.368 kb on - strand at 320.409 kb on - strand at 320.558 kb on + strand at 320.558 kb on + strand at 320.559 kb on - strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.681 kb on + strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.735 kb on - strand at 320.787 kb on + strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.848 kb on + strand at 320.849 kb on - strand at 320.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 317,940 + Pf1N1B4_5546 0.45 +0.6 317,948 + Pf1N1B4_5546 0.46 -0.4 317,948 + Pf1N1B4_5546 0.46 -2.4 317,985 + Pf1N1B4_5546 0.49 +2.2 317,998 + Pf1N1B4_5546 0.51 +0.7 317,998 + Pf1N1B4_5546 0.51 -0.0 317,999 - Pf1N1B4_5546 0.51 +0.6 317,999 - Pf1N1B4_5546 0.51 +1.2 317,999 - Pf1N1B4_5546 0.51 +0.4 318,105 + Pf1N1B4_5546 0.62 +0.2 318,130 + Pf1N1B4_5546 0.65 +0.7 318,131 - Pf1N1B4_5546 0.65 +0.7 318,167 + Pf1N1B4_5546 0.69 +0.5 318,249 + Pf1N1B4_5546 0.77 +1.7 318,249 + Pf1N1B4_5546 0.77 -0.8 318,249 + Pf1N1B4_5546 0.77 +0.2 318,249 + Pf1N1B4_5546 0.77 +1.0 318,250 - Pf1N1B4_5546 0.78 +0.2 318,253 + -0.8 318,253 + Pf1N1B4_5546 0.78 +0.2 318,315 + Pf1N1B4_5546 0.85 +1.0 318,315 + Pf1N1B4_5546 0.85 -0.8 318,315 + Pf1N1B4_5546 0.85 +1.5 318,315 + Pf1N1B4_5546 0.85 +1.2 318,316 - Pf1N1B4_5546 0.85 +2.3 318,358 + Pf1N1B4_5546 0.89 +0.7 318,358 + Pf1N1B4_5546 0.89 +0.7 318,358 + Pf1N1B4_5546 0.89 -0.3 318,358 + Pf1N1B4_5546 0.89 -0.3 318,358 + Pf1N1B4_5546 0.89 -1.4 318,359 - Pf1N1B4_5546 0.89 +2.1 318,359 - Pf1N1B4_5546 0.89 -0.4 318,359 - Pf1N1B4_5546 0.89 -0.8 318,359 - Pf1N1B4_5546 0.89 +0.7 318,432 + -0.3 318,440 + +0.7 318,610 - Pf1N1B4_5547 0.40 +1.0 318,651 - Pf1N1B4_5547 0.54 -0.8 318,896 + +2.2 318,897 - +0.8 318,920 + -0.3 318,986 - -0.1 318,986 - -0.3 318,986 - +0.2 318,986 - +1.2 319,027 + -0.4 319,028 - -0.1 319,028 - -0.1 319,028 - -0.5 319,028 - +0.7 319,044 + Pf1N1B4_5548 0.11 -0.4 319,060 - Pf1N1B4_5548 0.13 +0.7 319,060 - Pf1N1B4_5548 0.13 +0.2 319,060 - Pf1N1B4_5548 0.13 +1.5 319,182 + Pf1N1B4_5548 0.25 +0.7 319,360 - Pf1N1B4_5548 0.42 +1.6 319,408 + Pf1N1B4_5548 0.47 +1.0 319,408 + Pf1N1B4_5548 0.47 +2.1 319,408 + Pf1N1B4_5548 0.47 +2.0 319,408 + Pf1N1B4_5548 0.47 +2.3 319,502 + Pf1N1B4_5548 0.56 -0.8 319,503 - Pf1N1B4_5548 0.57 +1.2 319,503 - Pf1N1B4_5548 0.57 +0.6 319,596 + Pf1N1B4_5548 0.66 -0.3 319,611 + Pf1N1B4_5548 0.67 +0.7 319,611 + Pf1N1B4_5548 0.67 +0.2 319,612 - Pf1N1B4_5548 0.67 -0.4 319,627 + Pf1N1B4_5548 0.69 -1.4 319,627 + Pf1N1B4_5548 0.69 +0.7 319,627 + Pf1N1B4_5548 0.69 +2.3 319,627 + Pf1N1B4_5548 0.69 +1.2 319,628 - Pf1N1B4_5548 0.69 -1.6 319,716 + Pf1N1B4_5548 0.78 +0.2 319,730 - Pf1N1B4_5548 0.79 +1.5 319,779 + Pf1N1B4_5548 0.84 -0.3 319,779 + Pf1N1B4_5548 0.84 +0.7 319,779 + Pf1N1B4_5548 0.84 +1.5 319,779 + Pf1N1B4_5548 0.84 -1.8 319,780 - Pf1N1B4_5548 0.84 +0.6 319,858 + -0.3 319,858 + +0.9 319,859 - +0.2 319,925 - +1.0 319,925 - +0.1 319,925 - -0.1 319,926 + -0.8 319,926 + +0.7 319,926 + +0.5 319,926 + +1.5 319,926 + +0.1 319,926 + -1.3 319,926 + +1.2 319,926 + -0.0 319,927 - +1.2 319,927 - -0.8 319,927 - +0.2 319,927 - +1.0 319,927 - -1.1 319,947 + +1.2 319,948 - +0.6 319,954 - -0.8 319,954 - +2.2 319,954 - -0.8 319,954 - -0.3 319,966 + +1.7 319,971 + +1.5 319,975 - +1.7 319,975 - -0.3 320,021 + -0.4 320,022 - -0.3 320,135 + +0.7 320,135 + +1.3 320,135 + +1.7 320,135 + -0.1 320,135 + +0.7 320,135 + +1.5 320,135 + +0.7 320,136 - +1.6 320,136 - +1.2 320,136 - +1.3 320,136 - -0.3 320,136 - +0.6 320,174 + +0.7 320,174 + -0.8 320,175 - +3.3 320,175 - +1.2 320,178 + +1.7 320,178 + -0.8 320,178 + +1.2 320,178 + +1.2 320,178 + +0.2 320,179 - -2.4 320,179 - -0.0 320,200 - +0.3 320,255 + +0.6 320,256 - +0.7 320,277 - +3.2 320,282 - -1.0 320,339 + -1.8 320,339 + +0.2 320,340 - -0.6 320,365 + +0.7 320,365 + -0.8 320,365 + +1.9 320,366 - +1.0 320,366 - -1.8 320,366 - -0.3 320,366 - -0.8 320,366 - +1.7 320,367 + -0.3 320,367 + -0.8 320,368 - +1.5 320,409 - +0.0 320,558 + +0.5 320,558 + -0.0 320,559 - +0.7 320,622 + +0.4 320,622 + +1.4 320,622 + -1.3 320,623 - -1.8 320,623 - +2.2 320,623 - +0.2 320,681 + +0.7 320,682 - -0.8 320,682 - -1.8 320,682 - +1.2 320,685 + -1.3 320,685 + +1.3 320,685 + -0.8 320,685 + -0.5 320,685 + +0.1 320,685 + +1.3 320,686 - +0.5 320,686 - +1.7 320,686 - +0.5 320,686 - +0.6 320,686 - -0.4 320,686 - -0.8 320,735 - -0.8 320,787 + +1.1 320,788 - +0.4 320,788 - +1.2 320,848 + +2.0 320,849 - -0.8 320,935 - +0.5
Or see this region's nucleotide sequence