Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_497 and Pf1N1B4_498 overlap by 7 nucleotides Pf1N1B4_498 and Pf1N1B4_499 overlap by 1 nucleotides Pf1N1B4_499 and Pf1N1B4_500 are separated by 11 nucleotides
Pf1N1B4_497 - Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly, at 589,741 to 590,961
_497
Pf1N1B4_498 - Similar to TadZ/CpaE, associated with Flp pilus assembly, at 590,955 to 591,449
_498
Pf1N1B4_499 - Similar to TadZ/CpaE, associated with Flp pilus assembly, at 591,449 to 591,928
_499
Pf1N1B4_500 - Von Willebrand factor type A domain protein, associated with Flp pilus assembly, at 591,940 to 593,286
_500
Position (kb) 591
592 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.026 kb on + strand, within Pf1N1B4_498 at 591.027 kb on - strand, within Pf1N1B4_498 at 591.027 kb on - strand at 591.027 kb on - strand at 591.035 kb on + strand, within Pf1N1B4_498 at 591.100 kb on + strand, within Pf1N1B4_498 at 591.135 kb on - strand, within Pf1N1B4_498 at 591.175 kb on + strand, within Pf1N1B4_498 at 591.176 kb on - strand, within Pf1N1B4_498 at 591.176 kb on - strand, within Pf1N1B4_498 at 591.281 kb on + strand, within Pf1N1B4_498 at 591.332 kb on + strand, within Pf1N1B4_498 at 591.332 kb on + strand, within Pf1N1B4_498 at 591.332 kb on + strand, within Pf1N1B4_498 at 591.333 kb on - strand, within Pf1N1B4_498 at 591.333 kb on - strand, within Pf1N1B4_498 at 591.333 kb on - strand, within Pf1N1B4_498 at 591.333 kb on - strand, within Pf1N1B4_498 at 591.343 kb on + strand, within Pf1N1B4_498 at 591.343 kb on + strand, within Pf1N1B4_498 at 591.344 kb on - strand, within Pf1N1B4_498 at 591.344 kb on - strand, within Pf1N1B4_498 at 591.344 kb on - strand, within Pf1N1B4_498 at 591.346 kb on + strand, within Pf1N1B4_498 at 591.421 kb on + strand at 591.421 kb on + strand at 591.421 kb on + strand at 591.421 kb on + strand at 591.421 kb on + strand at 591.422 kb on - strand at 591.422 kb on - strand at 591.422 kb on - strand at 591.422 kb on - strand at 591.422 kb on - strand at 591.422 kb on - strand at 591.565 kb on + strand at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand at 591.565 kb on + strand at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.565 kb on + strand, within Pf1N1B4_499 at 591.566 kb on - strand, within Pf1N1B4_499 at 591.566 kb on - strand, within Pf1N1B4_499 at 591.566 kb on - strand, within Pf1N1B4_499 at 591.566 kb on - strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.567 kb on + strand, within Pf1N1B4_499 at 591.568 kb on - strand, within Pf1N1B4_499 at 591.568 kb on - strand, within Pf1N1B4_499 at 591.656 kb on - strand, within Pf1N1B4_499 at 591.672 kb on + strand, within Pf1N1B4_499 at 591.799 kb on - strand at 591.799 kb on - strand, within Pf1N1B4_499 at 591.799 kb on - strand, within Pf1N1B4_499 at 591.805 kb on - strand, within Pf1N1B4_499 at 591.911 kb on - strand at 591.941 kb on + strand at 591.978 kb on - strand at 591.987 kb on - strand at 592.112 kb on - strand, within Pf1N1B4_500 at 592.122 kb on - strand, within Pf1N1B4_500 at 592.122 kb on - strand, within Pf1N1B4_500 at 592.122 kb on - strand, within Pf1N1B4_500 at 592.284 kb on - strand at 592.349 kb on + strand, within Pf1N1B4_500 at 592.349 kb on + strand, within Pf1N1B4_500 at 592.349 kb on + strand, within Pf1N1B4_500 at 592.368 kb on + strand, within Pf1N1B4_500 at 592.382 kb on + strand, within Pf1N1B4_500 at 592.382 kb on + strand, within Pf1N1B4_500 at 592.383 kb on - strand, within Pf1N1B4_500 at 592.383 kb on - strand, within Pf1N1B4_500 at 592.383 kb on - strand, within Pf1N1B4_500 at 592.455 kb on - strand, within Pf1N1B4_500 at 592.455 kb on - strand, within Pf1N1B4_500 at 592.524 kb on + strand at 592.657 kb on + strand, within Pf1N1B4_500 at 592.657 kb on + strand, within Pf1N1B4_500 at 592.657 kb on + strand, within Pf1N1B4_500 at 592.861 kb on - strand, within Pf1N1B4_500 at 592.861 kb on - strand, within Pf1N1B4_500 at 592.861 kb on - strand, within Pf1N1B4_500 at 592.862 kb on + strand, within Pf1N1B4_500 at 592.862 kb on + strand, within Pf1N1B4_500 at 592.862 kb on + strand, within Pf1N1B4_500 at 592.863 kb on - strand, within Pf1N1B4_500 at 592.863 kb on - strand, within Pf1N1B4_500 at 592.863 kb on - strand, within Pf1N1B4_500 at 592.863 kb on - strand, within Pf1N1B4_500 at 592.863 kb on - strand, within Pf1N1B4_500 at 592.886 kb on + strand, within Pf1N1B4_500 at 592.887 kb on - strand, within Pf1N1B4_500 at 592.887 kb on - strand, within Pf1N1B4_500 at 592.887 kb on - strand, within Pf1N1B4_500
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 591,026 + Pf1N1B4_498 0.14 +0.9 591,026 + Pf1N1B4_498 0.14 -1.1 591,026 + Pf1N1B4_498 0.14 +0.3 591,026 + Pf1N1B4_498 0.14 +0.8 591,026 + Pf1N1B4_498 0.14 +1.5 591,026 + Pf1N1B4_498 0.14 +0.5 591,027 - Pf1N1B4_498 0.15 +1.5 591,027 - -2.7 591,027 - -1.1 591,035 + Pf1N1B4_498 0.16 -1.1 591,100 + Pf1N1B4_498 0.29 +0.5 591,135 - Pf1N1B4_498 0.36 +1.8 591,175 + Pf1N1B4_498 0.44 +1.3 591,176 - Pf1N1B4_498 0.45 -1.1 591,176 - Pf1N1B4_498 0.45 +0.4 591,281 + Pf1N1B4_498 0.66 +0.0 591,332 + Pf1N1B4_498 0.76 -2.1 591,332 + Pf1N1B4_498 0.76 -0.1 591,332 + Pf1N1B4_498 0.76 -3.1 591,333 - Pf1N1B4_498 0.76 -0.9 591,333 - Pf1N1B4_498 0.76 +0.9 591,333 - Pf1N1B4_498 0.76 +1.1 591,333 - Pf1N1B4_498 0.76 +2.0 591,343 + Pf1N1B4_498 0.78 +0.9 591,343 + Pf1N1B4_498 0.78 +2.1 591,344 - Pf1N1B4_498 0.79 +2.1 591,344 - Pf1N1B4_498 0.79 -1.1 591,344 - Pf1N1B4_498 0.79 -1.1 591,346 + Pf1N1B4_498 0.79 +0.9 591,421 + -1.1 591,421 + +1.0 591,421 + +1.0 591,421 + -0.2 591,421 + +0.3 591,422 - +0.5 591,422 - -1.1 591,422 - +0.5 591,422 - +0.2 591,422 - +1.9 591,422 - +1.7 591,565 + -0.1 591,565 + Pf1N1B4_499 0.24 -1.1 591,565 + Pf1N1B4_499 0.24 -0.1 591,565 + Pf1N1B4_499 0.24 -1.1 591,565 + Pf1N1B4_499 0.24 +0.7 591,565 + Pf1N1B4_499 0.24 -1.1 591,565 + +0.9 591,565 + +0.9 591,565 + Pf1N1B4_499 0.24 -1.7 591,565 + Pf1N1B4_499 0.24 -0.1 591,565 + Pf1N1B4_499 0.24 +0.7 591,566 - Pf1N1B4_499 0.24 -0.2 591,566 - Pf1N1B4_499 0.24 -0.5 591,566 - Pf1N1B4_499 0.24 +0.7 591,566 - Pf1N1B4_499 0.24 -0.1 591,567 + Pf1N1B4_499 0.25 -1.6 591,567 + Pf1N1B4_499 0.25 -0.1 591,567 + Pf1N1B4_499 0.25 +0.9 591,567 + Pf1N1B4_499 0.25 -0.2 591,567 + Pf1N1B4_499 0.25 -1.1 591,567 + Pf1N1B4_499 0.25 +0.5 591,568 - Pf1N1B4_499 0.25 -2.1 591,568 - Pf1N1B4_499 0.25 +0.5 591,656 - Pf1N1B4_499 0.43 +0.7 591,672 + Pf1N1B4_499 0.46 -1.1 591,799 - +1.5 591,799 - Pf1N1B4_499 0.73 -0.3 591,799 - Pf1N1B4_499 0.73 -1.9 591,805 - Pf1N1B4_499 0.74 -1.4 591,911 - -0.7 591,941 + +0.5 591,978 - +0.3 591,987 - -1.1 592,112 - Pf1N1B4_500 0.13 -0.5 592,122 - Pf1N1B4_500 0.14 +1.7 592,122 - Pf1N1B4_500 0.14 +0.9 592,122 - Pf1N1B4_500 0.14 -1.1 592,284 - +0.5 592,349 + Pf1N1B4_500 0.30 -1.1 592,349 + Pf1N1B4_500 0.30 -1.1 592,349 + Pf1N1B4_500 0.30 -0.3 592,368 + Pf1N1B4_500 0.32 -0.1 592,382 + Pf1N1B4_500 0.33 +1.4 592,382 + Pf1N1B4_500 0.33 +0.9 592,383 - Pf1N1B4_500 0.33 +0.5 592,383 - Pf1N1B4_500 0.33 +0.6 592,383 - Pf1N1B4_500 0.33 +0.9 592,455 - Pf1N1B4_500 0.38 +0.9 592,455 - Pf1N1B4_500 0.38 -1.1 592,524 + -0.5 592,657 + Pf1N1B4_500 0.53 -0.1 592,657 + Pf1N1B4_500 0.53 +0.3 592,657 + Pf1N1B4_500 0.53 -0.5 592,861 - Pf1N1B4_500 0.68 +0.2 592,861 - Pf1N1B4_500 0.68 -0.6 592,861 - Pf1N1B4_500 0.68 +1.7 592,862 + Pf1N1B4_500 0.68 +0.9 592,862 + Pf1N1B4_500 0.68 +0.5 592,862 + Pf1N1B4_500 0.68 -0.8 592,863 - Pf1N1B4_500 0.69 -0.1 592,863 - Pf1N1B4_500 0.69 +0.4 592,863 - Pf1N1B4_500 0.69 -1.1 592,863 - Pf1N1B4_500 0.69 -0.7 592,863 - Pf1N1B4_500 0.69 +0.1 592,886 + Pf1N1B4_500 0.70 +0.5 592,887 - Pf1N1B4_500 0.70 -0.5 592,887 - Pf1N1B4_500 0.70 +1.1 592,887 - Pf1N1B4_500 0.70 -0.5
Or see this region's nucleotide sequence