Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_383 and Pf1N1B4_384 are separated by 41 nucleotides Pf1N1B4_384 and Pf1N1B4_385 are separated by 158 nucleotides Pf1N1B4_385 and Pf1N1B4_386 are separated by 87 nucleotides
Pf1N1B4_383 - Collagenase and related proteases, at 453,554 to 454,156
_383
Pf1N1B4_384 - proteinase inhibitor, at 454,198 to 454,452
_384
Pf1N1B4_385 - Translation initiation factor 2 (IF-2; GTPase), at 454,611 to 455,078
_385
Pf1N1B4_386 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 455,166 to 456,605
_386
Position (kb) 454
455 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 453.228 kb on + strand at 453.229 kb on - strand at 453.316 kb on + strand at 453.317 kb on - strand at 453.327 kb on - strand at 453.327 kb on - strand at 453.327 kb on - strand at 453.355 kb on + strand at 453.471 kb on - strand at 453.471 kb on - strand at 453.471 kb on - strand at 453.537 kb on - strand at 453.545 kb on + strand at 453.545 kb on + strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.546 kb on - strand at 453.548 kb on - strand at 453.555 kb on + strand at 453.555 kb on + strand at 453.666 kb on + strand, within Pf1N1B4_383 at 453.666 kb on + strand at 453.666 kb on + strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.667 kb on - strand, within Pf1N1B4_383 at 453.732 kb on - strand, within Pf1N1B4_383 at 453.735 kb on - strand, within Pf1N1B4_383 at 453.756 kb on + strand, within Pf1N1B4_383 at 453.756 kb on + strand at 453.756 kb on + strand, within Pf1N1B4_383 at 453.756 kb on + strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.757 kb on - strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.870 kb on + strand, within Pf1N1B4_383 at 453.871 kb on - strand, within Pf1N1B4_383 at 453.871 kb on - strand, within Pf1N1B4_383 at 454.017 kb on + strand at 454.138 kb on + strand at 454.138 kb on + strand at 454.139 kb on - strand at 454.139 kb on - strand at 454.162 kb on + strand at 454.163 kb on - strand at 454.163 kb on - strand at 454.163 kb on - strand at 454.342 kb on - strand, within Pf1N1B4_384 at 454.351 kb on + strand, within Pf1N1B4_384 at 454.351 kb on + strand, within Pf1N1B4_384 at 454.352 kb on - strand, within Pf1N1B4_384 at 454.352 kb on - strand, within Pf1N1B4_384 at 454.516 kb on + strand at 454.613 kb on - strand at 454.819 kb on - strand, within Pf1N1B4_385 at 455.034 kb on + strand at 455.107 kb on + strand at 455.107 kb on + strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.441 kb on - strand at 455.441 kb on - strand, within Pf1N1B4_386 at 455.441 kb on - strand, within Pf1N1B4_386 at 455.441 kb on - strand, within Pf1N1B4_386
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 453,228 + +1.4 453,229 - +0.8 453,316 + +0.8 453,317 - +0.4 453,327 - -0.3 453,327 - +0.8 453,327 - +0.2 453,355 + -2.2 453,471 - -1.2 453,471 - +1.1 453,471 - +0.7 453,537 - -0.2 453,545 + -0.2 453,545 + +1.0 453,546 - -0.4 453,546 - -0.6 453,546 - +0.0 453,546 - +0.4 453,548 - +0.6 453,555 + +0.4 453,555 + +0.2 453,666 + Pf1N1B4_383 0.19 -0.2 453,666 + -1.2 453,666 + Pf1N1B4_383 0.19 -0.6 453,667 - Pf1N1B4_383 0.19 +0.4 453,667 - Pf1N1B4_383 0.19 +0.6 453,667 - Pf1N1B4_383 0.19 +1.8 453,667 - Pf1N1B4_383 0.19 -2.2 453,732 - Pf1N1B4_383 0.30 +0.5 453,735 - Pf1N1B4_383 0.30 +0.8 453,756 + Pf1N1B4_383 0.33 +0.4 453,756 + +1.6 453,756 + Pf1N1B4_383 0.33 +0.0 453,756 + Pf1N1B4_383 0.33 +0.7 453,757 - Pf1N1B4_383 0.34 +0.8 453,757 - Pf1N1B4_383 0.34 +0.1 453,757 - Pf1N1B4_383 0.34 +1.1 453,757 - Pf1N1B4_383 0.34 -2.8 453,870 + Pf1N1B4_383 0.52 -0.2 453,870 + Pf1N1B4_383 0.52 -1.9 453,870 + Pf1N1B4_383 0.52 -1.2 453,871 - Pf1N1B4_383 0.53 +0.8 453,871 - Pf1N1B4_383 0.53 +1.4 454,017 + -1.2 454,138 + +0.1 454,138 + -2.2 454,139 - +0.9 454,139 - +0.6 454,162 + -1.2 454,163 - +1.0 454,163 - +1.4 454,163 - +0.1 454,342 - Pf1N1B4_384 0.56 -1.2 454,351 + Pf1N1B4_384 0.60 -1.2 454,351 + Pf1N1B4_384 0.60 -0.8 454,352 - Pf1N1B4_384 0.60 -1.5 454,352 - Pf1N1B4_384 0.60 -0.2 454,516 + -0.7 454,613 - +0.2 454,819 - Pf1N1B4_385 0.44 -0.6 455,034 + +0.2 455,107 + +2.0 455,107 + -0.9 455,108 - +0.8 455,108 - +2.1 455,108 - -1.2 455,108 - +0.2 455,108 - -1.2 455,108 - +0.6 455,109 + -0.4 455,109 + +1.5 455,109 + -0.9 455,109 + +1.1 455,110 - +0.9 455,110 - +0.3 455,110 - +0.6 455,110 - +1.1 455,440 + Pf1N1B4_386 0.19 -2.8 455,440 + Pf1N1B4_386 0.19 +0.8 455,440 + Pf1N1B4_386 0.19 +0.8 455,440 + Pf1N1B4_386 0.19 -0.2 455,440 + Pf1N1B4_386 0.19 -0.0 455,440 + Pf1N1B4_386 0.19 +0.9 455,440 + Pf1N1B4_386 0.19 -0.2 455,441 - -1.8 455,441 - Pf1N1B4_386 0.19 +0.5 455,441 - Pf1N1B4_386 0.19 +2.1 455,441 - Pf1N1B4_386 0.19 -2.8
Or see this region's nucleotide sequence