Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_30 and Pf1N1B4_31 overlap by 4 nucleotides Pf1N1B4_31 and Pf1N1B4_32 are separated by 179 nucleotides Pf1N1B4_32 and Pf1N1B4_33 are separated by 174 nucleotides
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Pf1N1B4_32 - Transcriptional regulator, TetR family, at 37,197 to 37,817
_32
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Position (kb) 35
36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 34.913 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 35.033 kb on - strand, within Pf1N1B4_30 at 35.164 kb on + strand, within Pf1N1B4_30 at 35.165 kb on - strand, within Pf1N1B4_30 at 35.320 kb on - strand, within Pf1N1B4_30 at 35.463 kb on + strand, within Pf1N1B4_30 at 35.600 kb on - strand at 35.610 kb on + strand at 35.611 kb on - strand at 35.611 kb on - strand at 35.662 kb on - strand at 35.699 kb on + strand at 35.812 kb on - strand at 36.245 kb on + strand at 36.245 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.560 kb on - strand, within Pf1N1B4_31 at 36.750 kb on + strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 37.017 kb on + strand at 37.267 kb on + strand at 37.465 kb on + strand, within Pf1N1B4_32 at 37.482 kb on - strand at 37.558 kb on - strand, within Pf1N1B4_32 at 37.608 kb on - strand at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.687 kb on - strand, within Pf1N1B4_32 at 37.933 kb on + strand at 37.934 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 34,913 - Pf1N1B4_30 0.68 +0.3 34,913 - Pf1N1B4_30 0.68 -1.0 35,033 - Pf1N1B4_30 0.72 +2.0 35,164 + Pf1N1B4_30 0.76 -0.5 35,165 - Pf1N1B4_30 0.76 +0.7 35,320 - Pf1N1B4_30 0.81 +1.9 35,463 + Pf1N1B4_30 0.86 +0.7 35,600 - -0.3 35,610 + +1.3 35,611 - -1.3 35,611 - +0.3 35,662 - -0.1 35,699 + +1.2 35,812 - -1.3 36,245 + -1.9 36,245 + Pf1N1B4_31 0.31 -0.7 36,370 + Pf1N1B4_31 0.42 -2.3 36,370 + Pf1N1B4_31 0.42 -0.0 36,479 - Pf1N1B4_31 0.52 -0.3 36,479 - Pf1N1B4_31 0.52 -1.3 36,479 - Pf1N1B4_31 0.52 +0.7 36,479 - Pf1N1B4_31 0.52 +0.1 36,560 - Pf1N1B4_31 0.59 +1.5 36,750 + Pf1N1B4_31 0.76 -1.1 36,751 - Pf1N1B4_31 0.76 +0.3 36,751 - Pf1N1B4_31 0.76 +0.9 37,017 + +0.4 37,267 + -0.7 37,465 + Pf1N1B4_32 0.43 -0.3 37,482 - +0.3 37,558 - Pf1N1B4_32 0.58 -1.3 37,608 - -2.9 37,657 + Pf1N1B4_32 0.74 -2.0 37,657 + Pf1N1B4_32 0.74 +0.6 37,657 + Pf1N1B4_32 0.74 +0.3 37,658 - Pf1N1B4_32 0.74 +0.5 37,658 - +0.7 37,658 - -0.3 37,658 - -1.3 37,658 - Pf1N1B4_32 0.74 -0.1 37,658 - Pf1N1B4_32 0.74 -2.3 37,658 - Pf1N1B4_32 0.74 +1.7 37,658 - +1.0 37,687 - Pf1N1B4_32 0.79 +1.7 37,933 + +1.3 37,934 - +1.5
Or see this region's nucleotide sequence