Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2629 and Pf1N1B4_2630 overlap by 4 nucleotides Pf1N1B4_2630 and Pf1N1B4_2631 overlap by 4 nucleotides Pf1N1B4_2631 and Pf1N1B4_2632 overlap by 4 nucleotides
Pf1N1B4_2629 - Inner membrane protein YbhQ, at 2,966,782 to 2,967,768
_2629
Pf1N1B4_2630 - Cardiolipin synthetase (EC 2.7.8.-), at 2,967,765 to 2,969,051
_2630
Pf1N1B4_2631 - Endonuclease/Exonuclease/phosphatase family protein, at 2,969,048 to 2,969,845
_2631
Pf1N1B4_2632 - FIG003003: hypothetical protein, at 2,969,842 to 2,970,723
_2632
Position (kb)
2967
2968
2969
2970 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.838 kb on - strand at 2966.838 kb on - strand at 2966.851 kb on - strand at 2966.873 kb on + strand at 2966.873 kb on + strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.894 kb on - strand, within Pf1N1B4_2629 at 2967.036 kb on - strand at 2967.053 kb on - strand, within Pf1N1B4_2629 at 2967.320 kb on + strand, within Pf1N1B4_2629 at 2967.396 kb on - strand, within Pf1N1B4_2629 at 2967.396 kb on - strand, within Pf1N1B4_2629 at 2967.442 kb on - strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.455 kb on + strand, within Pf1N1B4_2629 at 2967.456 kb on - strand, within Pf1N1B4_2629 at 2967.456 kb on - strand, within Pf1N1B4_2629 at 2967.508 kb on - strand, within Pf1N1B4_2629 at 2967.508 kb on - strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.509 kb on + strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.510 kb on - strand, within Pf1N1B4_2629 at 2967.539 kb on + strand, within Pf1N1B4_2629 at 2967.539 kb on + strand, within Pf1N1B4_2629 at 2967.539 kb on + strand at 2967.539 kb on + strand, within Pf1N1B4_2629 at 2967.540 kb on - strand, within Pf1N1B4_2629 at 2967.540 kb on - strand, within Pf1N1B4_2629 at 2967.540 kb on - strand, within Pf1N1B4_2629 at 2967.658 kb on + strand, within Pf1N1B4_2629 at 2967.658 kb on + strand, within Pf1N1B4_2629 at 2967.658 kb on + strand, within Pf1N1B4_2629 at 2968.121 kb on - strand, within Pf1N1B4_2630 at 2968.121 kb on - strand, within Pf1N1B4_2630 at 2968.147 kb on + strand, within Pf1N1B4_2630 at 2968.153 kb on + strand, within Pf1N1B4_2630 at 2968.153 kb on + strand, within Pf1N1B4_2630 at 2968.153 kb on + strand, within Pf1N1B4_2630 at 2968.215 kb on + strand, within Pf1N1B4_2630 at 2968.215 kb on + strand, within Pf1N1B4_2630 at 2968.279 kb on + strand, within Pf1N1B4_2630 at 2968.279 kb on + strand, within Pf1N1B4_2630 at 2968.280 kb on - strand, within Pf1N1B4_2630 at 2968.330 kb on + strand, within Pf1N1B4_2630 at 2968.331 kb on - strand, within Pf1N1B4_2630 at 2968.372 kb on + strand, within Pf1N1B4_2630 at 2968.373 kb on - strand, within Pf1N1B4_2630 at 2968.384 kb on - strand, within Pf1N1B4_2630 at 2968.447 kb on + strand, within Pf1N1B4_2630 at 2968.447 kb on + strand, within Pf1N1B4_2630 at 2968.448 kb on - strand, within Pf1N1B4_2630 at 2968.532 kb on - strand, within Pf1N1B4_2630 at 2968.689 kb on + strand, within Pf1N1B4_2630 at 2968.689 kb on + strand, within Pf1N1B4_2630 at 2968.690 kb on - strand, within Pf1N1B4_2630 at 2968.712 kb on + strand at 2968.713 kb on - strand, within Pf1N1B4_2630 at 2968.729 kb on + strand at 2968.729 kb on + strand, within Pf1N1B4_2630 at 2968.729 kb on + strand, within Pf1N1B4_2630 at 2968.730 kb on - strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.912 kb on + strand, within Pf1N1B4_2630 at 2968.913 kb on - strand, within Pf1N1B4_2630 at 2968.913 kb on - strand, within Pf1N1B4_2630 at 2968.913 kb on - strand, within Pf1N1B4_2630 at 2968.913 kb on - strand, within Pf1N1B4_2630 at 2968.963 kb on + strand at 2968.964 kb on - strand at 2968.964 kb on - strand at 2968.964 kb on - strand at 2968.965 kb on + strand at 2968.965 kb on + strand at 2968.965 kb on + strand at 2968.966 kb on - strand at 2968.966 kb on - strand at 2968.966 kb on - strand at 2968.966 kb on - strand at 2968.966 kb on - strand at 2969.051 kb on + strand at 2969.051 kb on + strand at 2969.051 kb on + strand at 2969.052 kb on - strand at 2969.052 kb on - strand at 2969.052 kb on - strand at 2969.052 kb on - strand at 2969.052 kb on - strand at 2969.114 kb on + strand at 2969.114 kb on + strand at 2969.114 kb on + strand at 2969.114 kb on + strand at 2969.148 kb on + strand, within Pf1N1B4_2631 at 2969.148 kb on + strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.149 kb on - strand, within Pf1N1B4_2631 at 2969.191 kb on + strand, within Pf1N1B4_2631 at 2969.191 kb on + strand, within Pf1N1B4_2631 at 2969.191 kb on + strand, within Pf1N1B4_2631 at 2969.326 kb on + strand, within Pf1N1B4_2631 at 2969.326 kb on + strand, within Pf1N1B4_2631 at 2969.326 kb on + strand, within Pf1N1B4_2631 at 2969.326 kb on + strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.327 kb on - strand, within Pf1N1B4_2631 at 2969.599 kb on - strand, within Pf1N1B4_2631 at 2969.625 kb on + strand, within Pf1N1B4_2631 at 2969.626 kb on - strand, within Pf1N1B4_2631 at 2969.798 kb on - strand at 2969.798 kb on - strand at 2969.798 kb on - strand at 2969.966 kb on + strand, within Pf1N1B4_2632 at 2969.966 kb on + strand, within Pf1N1B4_2632 at 2969.967 kb on - strand, within Pf1N1B4_2632 at 2969.974 kb on + strand, within Pf1N1B4_2632 at 2969.974 kb on + strand, within Pf1N1B4_2632 at 2969.974 kb on + strand, within Pf1N1B4_2632 at 2969.974 kb on + strand, within Pf1N1B4_2632 at 2969.975 kb on - strand, within Pf1N1B4_2632 at 2969.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 2,966,837 + -0.7 2,966,837 + +1.4 2,966,837 + +0.0 2,966,837 + -0.1 2,966,837 + -0.5 2,966,837 + -0.7 2,966,837 + -0.4 2,966,838 - +0.6 2,966,838 - +1.2 2,966,851 - -2.0 2,966,873 + +1.4 2,966,873 + -0.7 2,966,874 - +3.2 2,966,874 - +2.9 2,966,874 - +1.6 2,966,874 - +1.3 2,966,894 - Pf1N1B4_2629 0.11 +0.3 2,967,036 - -0.7 2,967,053 - Pf1N1B4_2629 0.27 -0.7 2,967,320 + Pf1N1B4_2629 0.55 +0.6 2,967,396 - Pf1N1B4_2629 0.62 -1.7 2,967,396 - Pf1N1B4_2629 0.62 +1.1 2,967,442 - Pf1N1B4_2629 0.67 +1.9 2,967,455 + Pf1N1B4_2629 0.68 +0.4 2,967,455 + Pf1N1B4_2629 0.68 -1.3 2,967,455 + Pf1N1B4_2629 0.68 -0.7 2,967,455 + Pf1N1B4_2629 0.68 +0.1 2,967,455 + Pf1N1B4_2629 0.68 -0.1 2,967,455 + Pf1N1B4_2629 0.68 -1.7 2,967,456 - Pf1N1B4_2629 0.68 +0.1 2,967,456 - Pf1N1B4_2629 0.68 +0.9 2,967,508 - Pf1N1B4_2629 0.74 +2.3 2,967,508 - Pf1N1B4_2629 0.74 -0.1 2,967,509 + Pf1N1B4_2629 0.74 -0.7 2,967,509 + -0.1 2,967,509 + Pf1N1B4_2629 0.74 +0.3 2,967,509 + Pf1N1B4_2629 0.74 -0.7 2,967,509 + Pf1N1B4_2629 0.74 +0.9 2,967,509 + Pf1N1B4_2629 0.74 -0.7 2,967,509 + Pf1N1B4_2629 0.74 +0.9 2,967,509 + Pf1N1B4_2629 0.74 +2.3 2,967,509 + Pf1N1B4_2629 0.74 -1.7 2,967,509 + Pf1N1B4_2629 0.74 +0.5 2,967,509 + Pf1N1B4_2629 0.74 -0.7 2,967,509 + Pf1N1B4_2629 0.74 +2.1 2,967,509 + Pf1N1B4_2629 0.74 -2.3 2,967,509 + Pf1N1B4_2629 0.74 +0.3 2,967,509 + Pf1N1B4_2629 0.74 +0.0 2,967,509 + Pf1N1B4_2629 0.74 +0.2 2,967,510 - Pf1N1B4_2629 0.74 -1.5 2,967,510 - Pf1N1B4_2629 0.74 +0.9 2,967,510 - Pf1N1B4_2629 0.74 +1.3 2,967,510 - Pf1N1B4_2629 0.74 -1.7 2,967,510 - Pf1N1B4_2629 0.74 -0.7 2,967,510 - -0.7 2,967,510 - Pf1N1B4_2629 0.74 +0.5 2,967,510 - Pf1N1B4_2629 0.74 +2.3 2,967,510 - Pf1N1B4_2629 0.74 +0.5 2,967,510 - Pf1N1B4_2629 0.74 +1.3 2,967,510 - Pf1N1B4_2629 0.74 -0.0 2,967,510 - Pf1N1B4_2629 0.74 +1.3 2,967,510 - Pf1N1B4_2629 0.74 +0.3 2,967,539 + Pf1N1B4_2629 0.77 -0.7 2,967,539 + Pf1N1B4_2629 0.77 +0.5 2,967,539 + -1.7 2,967,539 + Pf1N1B4_2629 0.77 -0.0 2,967,540 - Pf1N1B4_2629 0.77 -0.7 2,967,540 - Pf1N1B4_2629 0.77 +0.3 2,967,540 - Pf1N1B4_2629 0.77 +0.6 2,967,658 + Pf1N1B4_2629 0.89 +1.3 2,967,658 + Pf1N1B4_2629 0.89 +2.1 2,967,658 + Pf1N1B4_2629 0.89 +0.3 2,968,121 - Pf1N1B4_2630 0.28 +0.3 2,968,121 - Pf1N1B4_2630 0.28 -0.0 2,968,147 + Pf1N1B4_2630 0.30 -0.7 2,968,153 + Pf1N1B4_2630 0.30 +0.9 2,968,153 + Pf1N1B4_2630 0.30 +0.4 2,968,153 + Pf1N1B4_2630 0.30 -0.0 2,968,215 + Pf1N1B4_2630 0.35 -0.7 2,968,215 + Pf1N1B4_2630 0.35 -0.2 2,968,279 + Pf1N1B4_2630 0.40 -0.3 2,968,279 + Pf1N1B4_2630 0.40 +1.6 2,968,280 - Pf1N1B4_2630 0.40 +0.9 2,968,330 + Pf1N1B4_2630 0.44 -0.7 2,968,331 - Pf1N1B4_2630 0.44 +0.6 2,968,372 + Pf1N1B4_2630 0.47 +0.4 2,968,373 - Pf1N1B4_2630 0.47 -1.3 2,968,384 - Pf1N1B4_2630 0.48 +1.6 2,968,447 + Pf1N1B4_2630 0.53 +1.5 2,968,447 + Pf1N1B4_2630 0.53 +0.1 2,968,448 - Pf1N1B4_2630 0.53 -0.7 2,968,532 - Pf1N1B4_2630 0.60 +0.1 2,968,689 + Pf1N1B4_2630 0.72 -0.7 2,968,689 + Pf1N1B4_2630 0.72 -0.1 2,968,690 - Pf1N1B4_2630 0.72 +0.1 2,968,712 + -0.1 2,968,713 - Pf1N1B4_2630 0.74 +0.1 2,968,729 + +0.3 2,968,729 + Pf1N1B4_2630 0.75 +0.9 2,968,729 + Pf1N1B4_2630 0.75 +0.7 2,968,730 - Pf1N1B4_2630 0.75 +2.3 2,968,912 + Pf1N1B4_2630 0.89 +1.3 2,968,912 + Pf1N1B4_2630 0.89 +1.3 2,968,912 + Pf1N1B4_2630 0.89 -0.1 2,968,912 + Pf1N1B4_2630 0.89 +1.1 2,968,912 + Pf1N1B4_2630 0.89 +1.4 2,968,912 + Pf1N1B4_2630 0.89 +1.7 2,968,912 + Pf1N1B4_2630 0.89 -0.7 2,968,913 - Pf1N1B4_2630 0.89 -0.2 2,968,913 - Pf1N1B4_2630 0.89 +0.1 2,968,913 - Pf1N1B4_2630 0.89 -0.1 2,968,913 - Pf1N1B4_2630 0.89 +0.0 2,968,963 + -0.3 2,968,964 - +0.9 2,968,964 - -0.1 2,968,964 - -0.1 2,968,965 + -0.7 2,968,965 + +0.3 2,968,965 + +0.4 2,968,966 - -0.1 2,968,966 - -1.0 2,968,966 - +1.4 2,968,966 - +0.3 2,968,966 - +0.3 2,969,051 + +0.3 2,969,051 + +2.7 2,969,051 + +0.1 2,969,052 - +1.4 2,969,052 - +1.0 2,969,052 - +0.3 2,969,052 - -2.3 2,969,052 - -0.7 2,969,114 + +0.3 2,969,114 + -0.7 2,969,114 + +1.6 2,969,114 + -0.1 2,969,148 + Pf1N1B4_2631 0.13 +0.9 2,969,148 + Pf1N1B4_2631 0.13 +0.3 2,969,149 - Pf1N1B4_2631 0.13 +1.6 2,969,149 - Pf1N1B4_2631 0.13 -0.1 2,969,149 - Pf1N1B4_2631 0.13 -0.7 2,969,149 - Pf1N1B4_2631 0.13 +1.6 2,969,149 - Pf1N1B4_2631 0.13 +0.9 2,969,149 - Pf1N1B4_2631 0.13 +0.9 2,969,149 - Pf1N1B4_2631 0.13 -0.4 2,969,149 - Pf1N1B4_2631 0.13 +0.5 2,969,149 - Pf1N1B4_2631 0.13 -0.4 2,969,149 - Pf1N1B4_2631 0.13 -1.3 2,969,191 + Pf1N1B4_2631 0.18 -1.7 2,969,191 + Pf1N1B4_2631 0.18 +0.3 2,969,191 + Pf1N1B4_2631 0.18 +0.3 2,969,326 + Pf1N1B4_2631 0.35 +1.6 2,969,326 + Pf1N1B4_2631 0.35 +0.3 2,969,326 + Pf1N1B4_2631 0.35 -1.1 2,969,326 + Pf1N1B4_2631 0.35 +0.9 2,969,327 - Pf1N1B4_2631 0.35 -0.7 2,969,327 - Pf1N1B4_2631 0.35 -1.0 2,969,327 - Pf1N1B4_2631 0.35 +1.9 2,969,327 - Pf1N1B4_2631 0.35 -1.1 2,969,327 - Pf1N1B4_2631 0.35 +1.3 2,969,327 - Pf1N1B4_2631 0.35 +0.7 2,969,599 - Pf1N1B4_2631 0.69 +0.3 2,969,625 + Pf1N1B4_2631 0.72 -0.7 2,969,626 - Pf1N1B4_2631 0.72 -0.2 2,969,798 - -0.7 2,969,798 - -1.7 2,969,798 - -1.7 2,969,966 + Pf1N1B4_2632 0.14 +0.0 2,969,966 + Pf1N1B4_2632 0.14 +2.3 2,969,967 - Pf1N1B4_2632 0.14 +1.3 2,969,974 + Pf1N1B4_2632 0.15 +0.1 2,969,974 + Pf1N1B4_2632 0.15 -0.4 2,969,974 + Pf1N1B4_2632 0.15 -0.7 2,969,974 + Pf1N1B4_2632 0.15 -0.7 2,969,975 - Pf1N1B4_2632 0.15 +0.6 2,969,975 - +0.3
Or see this region's nucleotide sequence