Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2481 and Pf1N1B4_2482 are separated by 194 nucleotides Pf1N1B4_2482 and Pf1N1B4_2483 are separated by 130 nucleotides Pf1N1B4_2483 and Pf1N1B4_2484 are separated by 24 nucleotides
Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931
_2481
Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027
_2482
Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922
_2483
Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150
_2484
Position (kb)
2816
2817
2818
2819 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.574 kb on - strand, within Pf1N1B4_2481 at 2815.831 kb on + strand, within Pf1N1B4_2481 at 2815.831 kb on + strand, within Pf1N1B4_2481 at 2816.411 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.692 kb on - strand, within Pf1N1B4_2482 at 2816.747 kb on - strand, within Pf1N1B4_2482 at 2816.747 kb on - strand, within Pf1N1B4_2482 at 2816.913 kb on + strand, within Pf1N1B4_2482 at 2816.913 kb on + strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.914 kb on - strand, within Pf1N1B4_2482 at 2816.959 kb on + strand, within Pf1N1B4_2482 at 2816.959 kb on + strand, within Pf1N1B4_2482 at 2816.959 kb on + strand, within Pf1N1B4_2482 at 2816.991 kb on + strand, within Pf1N1B4_2482 at 2816.991 kb on + strand, within Pf1N1B4_2482 at 2816.991 kb on + strand, within Pf1N1B4_2482 at 2816.992 kb on - strand, within Pf1N1B4_2482 at 2817.024 kb on + strand at 2817.024 kb on + strand, within Pf1N1B4_2482 at 2817.024 kb on + strand at 2817.024 kb on + strand, within Pf1N1B4_2482 at 2817.024 kb on + strand, within Pf1N1B4_2482 at 2817.024 kb on + strand, within Pf1N1B4_2482 at 2817.025 kb on - strand, within Pf1N1B4_2482 at 2817.025 kb on - strand, within Pf1N1B4_2482 at 2817.025 kb on - strand, within Pf1N1B4_2482 at 2817.025 kb on - strand, within Pf1N1B4_2482 at 2817.220 kb on + strand, within Pf1N1B4_2482 at 2817.220 kb on + strand, within Pf1N1B4_2482 at 2817.280 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.321 kb on + strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.322 kb on - strand, within Pf1N1B4_2482 at 2817.580 kb on + strand at 2817.580 kb on + strand, within Pf1N1B4_2482 at 2817.581 kb on - strand, within Pf1N1B4_2482 at 2817.606 kb on + strand, within Pf1N1B4_2482 at 2817.606 kb on + strand, within Pf1N1B4_2482 at 2817.615 kb on + strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.616 kb on - strand, within Pf1N1B4_2482 at 2817.799 kb on - strand, within Pf1N1B4_2482 at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.016 kb on + strand at 2818.017 kb on - strand at 2818.017 kb on - strand at 2818.017 kb on - strand at 2818.019 kb on + strand at 2818.019 kb on + strand at 2818.019 kb on + strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.020 kb on - strand at 2818.061 kb on - strand at 2818.061 kb on - strand at 2818.095 kb on + strand at 2818.096 kb on - strand at 2818.096 kb on - strand at 2818.134 kb on + strand at 2818.134 kb on + strand at 2818.135 kb on - strand at 2818.169 kb on + strand at 2818.423 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.910 kb on + strand at 2818.910 kb on + strand at 2818.911 kb on - strand at 2818.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 2,815,180 + Pf1N1B4_2481 0.23 +0.2 2,815,180 + Pf1N1B4_2481 0.23 +1.7 2,815,248 - Pf1N1B4_2481 0.30 +0.1 2,815,248 - Pf1N1B4_2481 0.30 +1.1 2,815,375 + Pf1N1B4_2481 0.43 -0.1 2,815,375 + Pf1N1B4_2481 0.43 +0.2 2,815,375 + Pf1N1B4_2481 0.43 +1.3 2,815,375 + Pf1N1B4_2481 0.43 +1.3 2,815,375 + Pf1N1B4_2481 0.43 -0.7 2,815,375 + Pf1N1B4_2481 0.43 +1.3 2,815,375 + Pf1N1B4_2481 0.43 -0.7 2,815,376 - Pf1N1B4_2481 0.43 +2.7 2,815,376 - Pf1N1B4_2481 0.43 +0.9 2,815,574 - Pf1N1B4_2481 0.63 +0.3 2,815,831 + Pf1N1B4_2481 0.90 +0.2 2,815,831 + Pf1N1B4_2481 0.90 -0.2 2,816,411 - Pf1N1B4_2482 0.15 +0.0 2,816,692 - Pf1N1B4_2482 0.30 -0.3 2,816,692 - Pf1N1B4_2482 0.30 +0.6 2,816,692 - Pf1N1B4_2482 0.30 +1.1 2,816,692 - Pf1N1B4_2482 0.30 +0.9 2,816,747 - Pf1N1B4_2482 0.33 +1.7 2,816,747 - Pf1N1B4_2482 0.33 -0.3 2,816,913 + Pf1N1B4_2482 0.41 +1.1 2,816,913 + Pf1N1B4_2482 0.41 +0.1 2,816,914 - Pf1N1B4_2482 0.41 +1.0 2,816,914 - Pf1N1B4_2482 0.41 -1.4 2,816,914 - Pf1N1B4_2482 0.41 +1.2 2,816,914 - Pf1N1B4_2482 0.41 +1.6 2,816,914 - Pf1N1B4_2482 0.41 +1.3 2,816,959 + Pf1N1B4_2482 0.44 -0.7 2,816,959 + Pf1N1B4_2482 0.44 -1.7 2,816,959 + Pf1N1B4_2482 0.44 +0.3 2,816,991 + Pf1N1B4_2482 0.45 -0.9 2,816,991 + Pf1N1B4_2482 0.45 +2.1 2,816,991 + Pf1N1B4_2482 0.45 +1.2 2,816,992 - Pf1N1B4_2482 0.46 +1.7 2,817,024 + +1.3 2,817,024 + Pf1N1B4_2482 0.47 +1.9 2,817,024 + -1.7 2,817,024 + Pf1N1B4_2482 0.47 +2.1 2,817,024 + Pf1N1B4_2482 0.47 -0.7 2,817,024 + Pf1N1B4_2482 0.47 +1.5 2,817,025 - Pf1N1B4_2482 0.47 -1.4 2,817,025 - Pf1N1B4_2482 0.47 +1.9 2,817,025 - Pf1N1B4_2482 0.47 -1.4 2,817,025 - Pf1N1B4_2482 0.47 +1.9 2,817,220 + Pf1N1B4_2482 0.58 -1.7 2,817,220 + Pf1N1B4_2482 0.58 -0.1 2,817,280 + Pf1N1B4_2482 0.61 -0.1 2,817,321 + Pf1N1B4_2482 0.63 -0.1 2,817,321 + Pf1N1B4_2482 0.63 -0.3 2,817,321 + Pf1N1B4_2482 0.63 -1.1 2,817,321 + Pf1N1B4_2482 0.63 -0.4 2,817,322 - Pf1N1B4_2482 0.63 -0.5 2,817,322 - Pf1N1B4_2482 0.63 +2.1 2,817,322 - Pf1N1B4_2482 0.63 +0.3 2,817,322 - Pf1N1B4_2482 0.63 +0.5 2,817,322 - Pf1N1B4_2482 0.63 +0.3 2,817,580 + +0.3 2,817,580 + Pf1N1B4_2482 0.76 -0.7 2,817,581 - Pf1N1B4_2482 0.76 +1.7 2,817,606 + Pf1N1B4_2482 0.78 +0.1 2,817,606 + Pf1N1B4_2482 0.78 +1.4 2,817,615 + Pf1N1B4_2482 0.78 +0.9 2,817,616 - Pf1N1B4_2482 0.78 -0.7 2,817,616 - Pf1N1B4_2482 0.78 +0.3 2,817,616 - Pf1N1B4_2482 0.78 +1.3 2,817,799 - Pf1N1B4_2482 0.88 +0.9 2,818,016 + -0.3 2,818,016 + +0.9 2,818,016 + -0.1 2,818,016 + +1.3 2,818,016 + +0.3 2,818,017 - +0.3 2,818,017 - -0.7 2,818,017 - +1.2 2,818,019 + +0.3 2,818,019 + +1.3 2,818,019 + +1.1 2,818,020 - -1.0 2,818,020 - -0.7 2,818,020 - +1.9 2,818,020 - +0.6 2,818,061 - -0.3 2,818,061 - -0.4 2,818,095 + +1.8 2,818,096 - +0.9 2,818,096 - +1.9 2,818,134 + +1.3 2,818,134 + +0.0 2,818,135 - +1.1 2,818,169 + +0.1 2,818,423 - Pf1N1B4_2483 0.35 +0.9 2,818,670 - Pf1N1B4_2483 0.67 +2.5 2,818,670 - Pf1N1B4_2483 0.67 +1.3 2,818,801 + Pf1N1B4_2483 0.84 +0.9 2,818,801 + -0.7 2,818,801 + Pf1N1B4_2483 0.84 +0.3 2,818,801 + Pf1N1B4_2483 0.84 +0.7 2,818,801 + Pf1N1B4_2483 0.84 +0.9 2,818,801 + Pf1N1B4_2483 0.84 +0.9 2,818,802 - Pf1N1B4_2483 0.84 +2.8 2,818,802 - Pf1N1B4_2483 0.84 +0.2 2,818,802 - Pf1N1B4_2483 0.84 +0.9 2,818,910 + +1.0 2,818,910 + +1.1 2,818,911 - -0.1 2,818,911 - -1.3
Or see this region's nucleotide sequence