Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_211

Experiment: Ammonium chloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_210 and Pf1N1B4_211 overlap by 1 nucleotidesPf1N1B4_211 and Pf1N1B4_212 overlap by 25 nucleotidesPf1N1B4_212 and Pf1N1B4_213 are separated by 122 nucleotides Pf1N1B4_210 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 257,922 to 259,661 _210 Pf1N1B4_211 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 259,661 to 260,527 _211 Pf1N1B4_212 - Thiol:disulfide interchange protein DsbG precursor, at 260,503 to 261,270 _212 Pf1N1B4_213 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 261,393 to 262,205 _213 Position (kb) 259 260 261Strain fitness (log2 ratio) -3 -2 -1 0 1at 258.855 kb on + strandat 258.855 kb on + strand, within Pf1N1B4_210at 258.855 kb on + strand, within Pf1N1B4_210at 258.856 kb on - strand, within Pf1N1B4_210at 258.856 kb on - strand, within Pf1N1B4_210at 258.856 kb on - strand, within Pf1N1B4_210at 258.856 kb on - strand, within Pf1N1B4_210at 258.856 kb on - strand, within Pf1N1B4_210at 258.926 kb on - strandat 259.108 kb on - strandat 259.184 kb on - strand, within Pf1N1B4_210at 259.251 kb on + strand, within Pf1N1B4_210at 259.381 kb on - strand, within Pf1N1B4_210at 259.384 kb on - strand, within Pf1N1B4_210at 259.384 kb on - strand, within Pf1N1B4_210at 259.465 kb on + strandat 259.466 kb on - strand, within Pf1N1B4_210at 259.466 kb on - strand, within Pf1N1B4_210at 259.466 kb on - strand, within Pf1N1B4_210at 259.466 kb on - strand, within Pf1N1B4_210at 259.466 kb on - strand, within Pf1N1B4_210at 259.529 kb on + strandat 259.529 kb on + strandat 259.530 kb on - strandat 259.530 kb on - strandat 259.530 kb on - strandat 259.672 kb on + strandat 259.699 kb on + strandat 259.700 kb on - strandat 259.847 kb on + strand, within Pf1N1B4_211at 259.848 kb on - strand, within Pf1N1B4_211at 259.949 kb on + strand, within Pf1N1B4_211at 259.949 kb on + strand, within Pf1N1B4_211at 259.949 kb on + strand, within Pf1N1B4_211at 259.949 kb on + strand, within Pf1N1B4_211at 259.949 kb on + strand, within Pf1N1B4_211at 259.950 kb on - strand, within Pf1N1B4_211at 259.998 kb on + strand, within Pf1N1B4_211at 259.998 kb on + strand, within Pf1N1B4_211at 259.999 kb on - strand, within Pf1N1B4_211at 260.044 kb on + strand, within Pf1N1B4_211at 260.044 kb on + strand, within Pf1N1B4_211at 260.045 kb on - strand, within Pf1N1B4_211at 260.045 kb on - strand, within Pf1N1B4_211at 260.045 kb on - strand, within Pf1N1B4_211at 260.048 kb on + strand, within Pf1N1B4_211at 260.048 kb on + strand, within Pf1N1B4_211at 260.049 kb on - strand, within Pf1N1B4_211at 260.049 kb on - strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.106 kb on + strand, within Pf1N1B4_211at 260.107 kb on - strand, within Pf1N1B4_211at 260.107 kb on - strand, within Pf1N1B4_211at 260.107 kb on - strand, within Pf1N1B4_211at 260.120 kb on + strand, within Pf1N1B4_211at 260.120 kb on + strand, within Pf1N1B4_211at 260.365 kb on + strand, within Pf1N1B4_211at 260.366 kb on - strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.375 kb on + strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.376 kb on - strand, within Pf1N1B4_211at 260.377 kb on + strand, within Pf1N1B4_211at 260.675 kb on - strand, within Pf1N1B4_212at 260.698 kb on + strand, within Pf1N1B4_212at 260.698 kb on + strand, within Pf1N1B4_212at 260.699 kb on - strand, within Pf1N1B4_212at 260.723 kb on + strand, within Pf1N1B4_212at 260.723 kb on + strand, within Pf1N1B4_212at 260.723 kb on + strand, within Pf1N1B4_212at 260.723 kb on + strand, within Pf1N1B4_212at 260.723 kb on + strand, within Pf1N1B4_212at 260.724 kb on - strand, within Pf1N1B4_212at 260.724 kb on - strand, within Pf1N1B4_212at 260.724 kb on - strand, within Pf1N1B4_212at 260.724 kb on - strand, within Pf1N1B4_212at 260.724 kb on - strand, within Pf1N1B4_212at 260.792 kb on - strand, within Pf1N1B4_212at 260.792 kb on - strand, within Pf1N1B4_212at 260.878 kb on + strand, within Pf1N1B4_212at 260.879 kb on - strand, within Pf1N1B4_212at 260.906 kb on - strand, within Pf1N1B4_212at 261.187 kb on + strand, within Pf1N1B4_212at 261.188 kb on - strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.191 kb on + strand, within Pf1N1B4_212at 261.192 kb on - strand, within Pf1N1B4_212at 261.192 kb on - strandat 261.192 kb on - strand, within Pf1N1B4_212at 261.192 kb on - strand, within Pf1N1B4_212at 261.268 kb on + strandat 261.268 kb on + strandat 261.268 kb on + strandat 261.268 kb on + strandat 261.269 kb on - strandat 261.272 kb on + strandat 261.273 kb on - strandat 261.342 kb on + strandat 261.343 kb on - strandat 261.385 kb on - strandat 261.394 kb on + strandat 261.462 kb on - strandat 261.462 kb on - strandat 261.487 kb on + strand, within Pf1N1B4_213at 261.488 kb on - strand, within Pf1N1B4_213

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N)
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258,855 + -1.3
258,855 + Pf1N1B4_210 0.54 -1.3
258,855 + Pf1N1B4_210 0.54 +0.6
258,856 - Pf1N1B4_210 0.54 -1.0
258,856 - Pf1N1B4_210 0.54 -0.7
258,856 - Pf1N1B4_210 0.54 -1.0
258,856 - Pf1N1B4_210 0.54 -1.3
258,856 - Pf1N1B4_210 0.54 -0.3
258,926 - -1.3
259,108 - -0.3
259,184 - Pf1N1B4_210 0.73 -2.6
259,251 + Pf1N1B4_210 0.76 +1.3
259,381 - Pf1N1B4_210 0.84 -0.3
259,384 - Pf1N1B4_210 0.84 -2.3
259,384 - Pf1N1B4_210 0.84 +0.3
259,465 + +0.3
259,466 - Pf1N1B4_210 0.89 +0.1
259,466 - Pf1N1B4_210 0.89 +1.6
259,466 - Pf1N1B4_210 0.89 -2.8
259,466 - Pf1N1B4_210 0.89 -0.3
259,466 - Pf1N1B4_210 0.89 +0.7
259,529 + +0.1
259,529 + +1.6
259,530 - -0.9
259,530 - +0.1
259,530 - -1.3
259,672 + -0.9
259,699 + +1.1
259,700 - +0.2
259,847 + Pf1N1B4_211 0.21 +0.3
259,848 - Pf1N1B4_211 0.22 -0.4
259,949 + Pf1N1B4_211 0.33 -0.4
259,949 + Pf1N1B4_211 0.33 -1.3
259,949 + Pf1N1B4_211 0.33 -0.3
259,949 + Pf1N1B4_211 0.33 -0.9
259,949 + Pf1N1B4_211 0.33 -0.5
259,950 - Pf1N1B4_211 0.33 -0.7
259,998 + Pf1N1B4_211 0.39 -0.3
259,998 + Pf1N1B4_211 0.39 -0.9
259,999 - Pf1N1B4_211 0.39 +0.3
260,044 + Pf1N1B4_211 0.44 -1.3
260,044 + Pf1N1B4_211 0.44 -0.7
260,045 - Pf1N1B4_211 0.44 -0.3
260,045 - Pf1N1B4_211 0.44 +0.9
260,045 - Pf1N1B4_211 0.44 -2.1
260,048 + Pf1N1B4_211 0.45 -0.7
260,048 + Pf1N1B4_211 0.45 -0.1
260,049 - Pf1N1B4_211 0.45 +0.4
260,049 - Pf1N1B4_211 0.45 +0.1
260,106 + Pf1N1B4_211 0.51 -0.3
260,106 + Pf1N1B4_211 0.51 +0.0
260,106 + Pf1N1B4_211 0.51 -2.1
260,106 + Pf1N1B4_211 0.51 -1.7
260,106 + Pf1N1B4_211 0.51 -1.3
260,106 + Pf1N1B4_211 0.51 +0.3
260,106 + Pf1N1B4_211 0.51 +0.9
260,106 + Pf1N1B4_211 0.51 +0.4
260,107 - Pf1N1B4_211 0.51 +0.9
260,107 - Pf1N1B4_211 0.51 -1.6
260,107 - Pf1N1B4_211 0.51 -1.3
260,120 + Pf1N1B4_211 0.53 -0.0
260,120 + Pf1N1B4_211 0.53 -1.3
260,365 + Pf1N1B4_211 0.81 +0.5
260,366 - Pf1N1B4_211 0.81 -1.3
260,375 + Pf1N1B4_211 0.82 +0.1
260,375 + Pf1N1B4_211 0.82 -0.5
260,375 + Pf1N1B4_211 0.82 -0.9
260,375 + Pf1N1B4_211 0.82 -0.5
260,375 + Pf1N1B4_211 0.82 -0.8
260,375 + Pf1N1B4_211 0.82 -0.3
260,375 + Pf1N1B4_211 0.82 -0.4
260,375 + Pf1N1B4_211 0.82 -0.4
260,375 + Pf1N1B4_211 0.82 +1.1
260,375 + Pf1N1B4_211 0.82 +1.2
260,375 + Pf1N1B4_211 0.82 +0.3
260,375 + Pf1N1B4_211 0.82 +1.1
260,375 + Pf1N1B4_211 0.82 -0.0
260,375 + Pf1N1B4_211 0.82 +1.6
260,375 + Pf1N1B4_211 0.82 +0.7
260,375 + Pf1N1B4_211 0.82 -1.3
260,376 - Pf1N1B4_211 0.82 -0.1
260,376 - Pf1N1B4_211 0.82 +0.9
260,376 - Pf1N1B4_211 0.82 +0.2
260,376 - Pf1N1B4_211 0.82 -0.8
260,376 - Pf1N1B4_211 0.82 +0.1
260,376 - Pf1N1B4_211 0.82 -0.8
260,376 - Pf1N1B4_211 0.82 -0.8
260,376 - Pf1N1B4_211 0.82 -1.3
260,376 - Pf1N1B4_211 0.82 -1.5
260,376 - Pf1N1B4_211 0.82 +1.2
260,376 - Pf1N1B4_211 0.82 -0.0
260,376 - Pf1N1B4_211 0.82 -1.5
260,376 - Pf1N1B4_211 0.82 +0.1
260,376 - Pf1N1B4_211 0.82 -1.3
260,376 - Pf1N1B4_211 0.82 +0.1
260,376 - Pf1N1B4_211 0.82 -0.6
260,376 - Pf1N1B4_211 0.82 +1.1
260,377 + Pf1N1B4_211 0.83 -0.9
260,675 - Pf1N1B4_212 0.22 +0.3
260,698 + Pf1N1B4_212 0.25 +1.2
260,698 + Pf1N1B4_212 0.25 +1.7
260,699 - Pf1N1B4_212 0.26 -0.8
260,723 + Pf1N1B4_212 0.29 +1.1
260,723 + Pf1N1B4_212 0.29 -0.9
260,723 + Pf1N1B4_212 0.29 -0.3
260,723 + Pf1N1B4_212 0.29 +0.2
260,723 + Pf1N1B4_212 0.29 +0.1
260,724 - Pf1N1B4_212 0.29 -0.7
260,724 - Pf1N1B4_212 0.29 +0.2
260,724 - Pf1N1B4_212 0.29 +0.1
260,724 - Pf1N1B4_212 0.29 -1.3
260,724 - Pf1N1B4_212 0.29 +1.0
260,792 - Pf1N1B4_212 0.38 +0.3
260,792 - Pf1N1B4_212 0.38 +1.3
260,878 + Pf1N1B4_212 0.49 +0.1
260,879 - Pf1N1B4_212 0.49 -0.1
260,906 - Pf1N1B4_212 0.52 -0.5
261,187 + Pf1N1B4_212 0.89 +0.3
261,188 - Pf1N1B4_212 0.89 -0.3
261,191 + Pf1N1B4_212 0.90 +1.2
261,191 + Pf1N1B4_212 0.90 +0.3
261,191 + Pf1N1B4_212 0.90 +0.1
261,191 + Pf1N1B4_212 0.90 +1.2
261,191 + Pf1N1B4_212 0.90 +0.1
261,191 + Pf1N1B4_212 0.90 +0.3
261,191 + Pf1N1B4_212 0.90 -0.8
261,192 - Pf1N1B4_212 0.90 -0.8
261,192 - +0.3
261,192 - Pf1N1B4_212 0.90 +1.5
261,192 - Pf1N1B4_212 0.90 +0.3
261,268 + -0.5
261,268 + +1.4
261,268 + +0.2
261,268 + +0.3
261,269 - +0.9
261,272 + -0.3
261,273 - +0.7
261,342 + +0.1
261,343 - +1.3
261,385 - +0.5
261,394 + -1.3
261,462 - -0.7
261,462 - +1.7
261,487 + Pf1N1B4_213 0.12 -0.8
261,488 - Pf1N1B4_213 0.12 +1.5

Or see this region's nucleotide sequence