Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1330 and Pf1N1B4_1331 are separated by 242 nucleotides Pf1N1B4_1331 and Pf1N1B4_1332 overlap by 8 nucleotides
Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881
_1330
Pf1N1B4_1331 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 1,526,124 to 1,527,335
_1331
Pf1N1B4_1332 - Cobalt-precorrin-6 synthase, anaerobic, at 1,527,328 to 1,528,425
_1332
Position (kb)
1526
1527
1528 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1525.145 kb on - strand at 1525.201 kb on - strand at 1525.201 kb on - strand at 1525.208 kb on - strand at 1525.236 kb on - strand at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.383 kb on + strand, within Pf1N1B4_1330 at 1525.384 kb on - strand, within Pf1N1B4_1330 at 1525.448 kb on + strand, within Pf1N1B4_1330 at 1525.464 kb on + strand, within Pf1N1B4_1330 at 1525.729 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.759 kb on + strand, within Pf1N1B4_1330 at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.882 kb on - strand at 1525.975 kb on + strand at 1526.016 kb on + strand at 1526.016 kb on + strand at 1526.339 kb on - strand, within Pf1N1B4_1331 at 1526.482 kb on + strand, within Pf1N1B4_1331 at 1526.483 kb on - strand, within Pf1N1B4_1331 at 1526.914 kb on + strand at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1527.116 kb on - strand, within Pf1N1B4_1331 at 1527.116 kb on - strand, within Pf1N1B4_1331 at 1527.123 kb on - strand, within Pf1N1B4_1331 at 1527.123 kb on - strand, within Pf1N1B4_1331 at 1527.217 kb on - strand at 1527.299 kb on + strand at 1527.299 kb on + strand at 1527.300 kb on - strand at 1527.300 kb on - strand at 1527.757 kb on + strand, within Pf1N1B4_1332 at 1527.901 kb on + strand, within Pf1N1B4_1332 at 1527.902 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.252 kb on - strand, within Pf1N1B4_1332
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,525,145 - +0.8 1,525,201 - -0.1 1,525,201 - +0.3 1,525,208 - +1.1 1,525,236 - +1.6 1,525,377 - Pf1N1B4_1330 0.23 +0.3 1,525,377 - Pf1N1B4_1330 0.23 +2.1 1,525,377 - Pf1N1B4_1330 0.23 -0.1 1,525,383 + Pf1N1B4_1330 0.24 +0.6 1,525,384 - Pf1N1B4_1330 0.24 +0.3 1,525,448 + Pf1N1B4_1330 0.34 -0.5 1,525,464 + Pf1N1B4_1330 0.36 +0.9 1,525,729 - Pf1N1B4_1330 0.77 +3.6 1,525,731 - Pf1N1B4_1330 0.77 +0.7 1,525,731 - Pf1N1B4_1330 0.77 +0.9 1,525,759 + Pf1N1B4_1330 0.81 +0.9 1,525,825 - +0.5 1,525,825 - +0.9 1,525,825 - +0.9 1,525,882 - -0.7 1,525,975 + -0.4 1,526,016 + +0.6 1,526,016 + -0.7 1,526,339 - Pf1N1B4_1331 0.18 -1.4 1,526,482 + Pf1N1B4_1331 0.30 -1.7 1,526,483 - Pf1N1B4_1331 0.30 +0.8 1,526,914 + -1.7 1,526,915 - Pf1N1B4_1331 0.65 -1.5 1,526,915 - Pf1N1B4_1331 0.65 +0.9 1,526,915 - Pf1N1B4_1331 0.65 +1.1 1,527,116 - Pf1N1B4_1331 0.82 -1.0 1,527,116 - Pf1N1B4_1331 0.82 -1.1 1,527,123 - Pf1N1B4_1331 0.82 +1.3 1,527,123 - Pf1N1B4_1331 0.82 -1.5 1,527,217 - -0.7 1,527,299 + -1.7 1,527,299 + -0.7 1,527,300 - -0.7 1,527,300 - -0.1 1,527,757 + Pf1N1B4_1332 0.39 -0.7 1,527,901 + Pf1N1B4_1332 0.52 +0.4 1,527,902 - Pf1N1B4_1332 0.52 +0.0 1,528,007 - Pf1N1B4_1332 0.62 +0.3 1,528,007 - Pf1N1B4_1332 0.62 +0.4 1,528,252 - Pf1N1B4_1332 0.84 -3.0
Or see this region's nucleotide sequence