Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16900

Experiment: Glycerol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16895 and BWI76_RS16900 are separated by 74 nucleotidesBWI76_RS16900 and BWI76_RS16905 overlap by 4 nucleotidesBWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides BWI76_RS16895: BWI76_RS16895 - 3-(3-hydroxy-phenyl)propionate transporter MhpT, at 3,503,327 to 3,504,523 _RS16895 BWI76_RS16900: BWI76_RS16900 - 4-hydroxy-2-oxovalerate aldolase, at 3,504,598 to 3,505,614 _RS16900 BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561 _RS16905 BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360 _RS16910 Position (kb) 3504 3505 3506Strain fitness (log2 ratio) -1 0 1 2at 3504.087 kb on + strand, within BWI76_RS16895at 3504.221 kb on + strand, within BWI76_RS16895at 3504.460 kb on + strandat 3504.836 kb on + strand, within BWI76_RS16900at 3505.512 kb on - strand, within BWI76_RS16900at 3506.110 kb on - strand, within BWI76_RS16905at 3506.206 kb on + strand, within BWI76_RS16905at 3506.232 kb on + strand, within BWI76_RS16905at 3506.287 kb on - strand, within BWI76_RS16905at 3506.575 kb on - strandat 3506.594 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycerol (C)
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3,504,087 + BWI76_RS16895 0.63 -1.4
3,504,221 + BWI76_RS16895 0.75 +2.0
3,504,460 + +0.3
3,504,836 + BWI76_RS16900 0.23 -0.7
3,505,512 - BWI76_RS16900 0.90 +1.6
3,506,110 - BWI76_RS16905 0.52 +0.7
3,506,206 + BWI76_RS16905 0.63 +0.1
3,506,232 + BWI76_RS16905 0.65 +0.5
3,506,287 - BWI76_RS16905 0.71 +0.2
3,506,575 - +0.8
3,506,594 - -1.4

Or see this region's nucleotide sequence