Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16805

Experiment: Glycerol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16795 and BWI76_RS16800 overlap by 14 nucleotidesBWI76_RS16800 and BWI76_RS16805 are separated by 65 nucleotidesBWI76_RS16805 and BWI76_RS16810 are separated by 95 nucleotidesBWI76_RS16810 and BWI76_RS16815 overlap by 4 nucleotides BWI76_RS16795: BWI76_RS16795 - amino acid ABC transporter ATPase, at 3,482,646 to 3,483,368 _RS16795 BWI76_RS16800: BWI76_RS16800 - amino acid ABC transporter permease, at 3,483,355 to 3,484,119 _RS16800 BWI76_RS16805: BWI76_RS16805 - basic amino acid ABC transporter substrate-binding protein, at 3,484,185 to 3,484,952 _RS16805 BWI76_RS16810: BWI76_RS16810 - RpiR family transcriptional regulator, at 3,485,048 to 3,485,947 _RS16810 BWI76_RS16815: BWI76_RS16815 - periplasmic binding protein, at 3,485,944 to 3,486,891 _RS16815 Position (kb) 3484 3485Strain fitness (log2 ratio) -1 0 1at 3483.505 kb on - strand, within BWI76_RS16800at 3484.146 kb on + strandat 3484.161 kb on + strandat 3484.721 kb on - strand, within BWI76_RS16805at 3484.881 kb on + strandat 3484.949 kb on - strandat 3485.168 kb on + strand, within BWI76_RS16810at 3485.322 kb on - strand, within BWI76_RS16810at 3485.639 kb on - strand, within BWI76_RS16810at 3485.694 kb on - strand, within BWI76_RS16810

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycerol (C)
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3,483,505 - BWI76_RS16800 0.20 -0.1
3,484,146 + +0.6
3,484,161 + -0.9
3,484,721 - BWI76_RS16805 0.70 +0.5
3,484,881 + +0.3
3,484,949 - -0.4
3,485,168 + BWI76_RS16810 0.13 -0.2
3,485,322 - BWI76_RS16810 0.30 +1.3
3,485,639 - BWI76_RS16810 0.66 -0.4
3,485,694 - BWI76_RS16810 0.72 +0.3

Or see this region's nucleotide sequence