Experiment: L-aspartic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3032 and BT3033 are separated by 5 nucleotides BT3033 and BT3034 overlap by 4 nucleotides BT3034 and BT3035 are separated by 105 nucleotides
BT3032: BT3032 - conserved hypothetical protein, with a weak acyltransferase domain (NCBI ptt file), at 3,838,872 to 3,840,008
BT3032
BT3033: BT3033 - DNA topoisomerase IV subunit B (NCBI ptt file), at 3,840,014 to 3,841,891
BT3033
BT3034: BT3034 - phosphopantetheine adenylyltransferase (NCBI ptt file), at 3,841,888 to 3,842,343
BT3034
BT3035: BT3035 - carboxy-terminal processing protease precursor (NCBI ptt file), at 3,842,449 to 3,844,038
BT3035
Position (kb) 3840
3841
3842 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3840.013 kb on + strand at 3840.013 kb on + strand at 3840.013 kb on + strand at 3842.376 kb on - strand at 3842.376 kb on - strand at 3842.402 kb on - strand at 3842.472 kb on - strand at 3842.474 kb on - strand at 3842.594 kb on + strand at 3842.874 kb on + strand, within BT3035 at 3842.875 kb on - strand, within BT3035
Per-strain Table
Position Strand Gene LocusTag Fraction L-aspartic (N) remove 3,840,013 + -1.6 3,840,013 + -1.6 3,840,013 + +0.4 3,842,376 - -5.0 3,842,376 - -1.4 3,842,402 - -1.6 3,842,472 - -3.0 3,842,474 - -4.6 3,842,594 + -0.9 3,842,874 + BT3035 0.27 -3.6 3,842,875 - BT3035 0.27 -5.7
Or see this region's nucleotide sequence