Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36445 and RR42_RS36450 are separated by 69 nucleotides RR42_RS36450 and RR42_RS36455 are separated by 19 nucleotides RR42_RS36455 and RR42_RS36460 are separated by 13 nucleotides
RR42_RS36445: RR42_RS36445 - acetyl-CoA acetyltransferase, at 3,631,714 to 3,632,922
_RS36445
RR42_RS36450: RR42_RS36450 - acyl dehydratase, at 3,632,992 to 3,633,453
_RS36450
RR42_RS36455: RR42_RS36455 - acyl-CoA dehydrogenase, at 3,633,473 to 3,634,633
_RS36455
RR42_RS36460: RR42_RS36460 - acyl-CoA dehydrogenase, at 3,634,647 to 3,635,648
_RS36460
Position (kb)
3633
3634
3635 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3632.893 kb on + strand at 3632.894 kb on - strand at 3632.913 kb on + strand at 3632.914 kb on - strand at 3632.914 kb on - strand at 3632.914 kb on - strand at 3632.944 kb on - strand at 3632.948 kb on + strand at 3632.948 kb on + strand at 3632.948 kb on + strand at 3632.949 kb on - strand at 3632.949 kb on - strand at 3633.031 kb on + strand at 3633.031 kb on + strand at 3633.032 kb on - strand at 3633.203 kb on - strand, within RR42_RS36450 at 3633.386 kb on - strand, within RR42_RS36450 at 3633.421 kb on + strand at 3633.421 kb on + strand at 3633.422 kb on - strand at 3633.827 kb on + strand, within RR42_RS36455 at 3633.827 kb on + strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455 at 3633.995 kb on + strand, within RR42_RS36455 at 3633.996 kb on - strand, within RR42_RS36455 at 3633.996 kb on - strand, within RR42_RS36455 at 3634.177 kb on - strand, within RR42_RS36455 at 3634.251 kb on - strand, within RR42_RS36455 at 3634.273 kb on + strand, within RR42_RS36455 at 3634.324 kb on + strand, within RR42_RS36455 at 3634.325 kb on - strand, within RR42_RS36455 at 3634.565 kb on + strand at 3634.565 kb on + strand at 3634.566 kb on - strand at 3634.652 kb on + strand at 3634.652 kb on + strand at 3635.247 kb on + strand, within RR42_RS36460 at 3635.247 kb on + strand, within RR42_RS36460 at 3635.247 kb on + strand, within RR42_RS36460 at 3635.248 kb on - strand, within RR42_RS36460 at 3635.248 kb on - strand, within RR42_RS36460 at 3635.493 kb on + strand, within RR42_RS36460 at 3635.493 kb on + strand, within RR42_RS36460 at 3635.508 kb on + strand, within RR42_RS36460 at 3635.508 kb on + strand, within RR42_RS36460 at 3635.509 kb on - strand, within RR42_RS36460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,632,893 + +0.5 3,632,894 - +0.6 3,632,913 + +1.3 3,632,914 - +0.2 3,632,914 - +0.1 3,632,914 - +1.9 3,632,944 - -0.3 3,632,948 + -0.6 3,632,948 + +1.7 3,632,948 + -1.2 3,632,949 - -0.5 3,632,949 - +0.1 3,633,031 + -1.9 3,633,031 + +0.0 3,633,032 - -0.2 3,633,203 - RR42_RS36450 0.46 -1.5 3,633,386 - RR42_RS36450 0.85 +1.0 3,633,421 + -1.4 3,633,421 + -0.3 3,633,422 - +0.4 3,633,827 + RR42_RS36455 0.30 +0.5 3,633,827 + RR42_RS36455 0.30 -0.8 3,633,828 - RR42_RS36455 0.31 +0.5 3,633,828 - RR42_RS36455 0.31 -0.1 3,633,828 - RR42_RS36455 0.31 -0.1 3,633,995 + RR42_RS36455 0.45 -0.9 3,633,996 - RR42_RS36455 0.45 -0.2 3,633,996 - RR42_RS36455 0.45 -0.2 3,634,177 - RR42_RS36455 0.61 -0.4 3,634,251 - RR42_RS36455 0.67 +0.4 3,634,273 + RR42_RS36455 0.69 +0.3 3,634,324 + RR42_RS36455 0.73 +0.5 3,634,325 - RR42_RS36455 0.73 +0.1 3,634,565 + -0.2 3,634,565 + -0.0 3,634,566 - +2.3 3,634,652 + -1.9 3,634,652 + +1.6 3,635,247 + RR42_RS36460 0.60 -1.2 3,635,247 + RR42_RS36460 0.60 +0.7 3,635,247 + RR42_RS36460 0.60 -0.1 3,635,248 - RR42_RS36460 0.60 +0.4 3,635,248 - RR42_RS36460 0.60 -0.9 3,635,493 + RR42_RS36460 0.84 +0.1 3,635,493 + RR42_RS36460 0.84 +0.1 3,635,508 + RR42_RS36460 0.86 +0.4 3,635,508 + RR42_RS36460 0.86 -0.7 3,635,509 - RR42_RS36460 0.86 -0.4
Or see this region's nucleotide sequence