Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35225 and RR42_RS35230 are separated by 19 nucleotides RR42_RS35230 and RR42_RS35235 overlap by 1 nucleotides RR42_RS35235 and RR42_RS35240 are separated by 445 nucleotides
RR42_RS35225: RR42_RS35225 - peptide ABC transporter substrate-binding protein, at 3,355,246 to 3,356,958
_RS35225
RR42_RS35230: RR42_RS35230 - ABC transporter permease, at 3,356,978 to 3,357,913
_RS35230
RR42_RS35235: RR42_RS35235 - glutathione ABC transporter permease, at 3,357,913 to 3,358,869
_RS35235
RR42_RS35240: RR42_RS35240 - acyl-CoA dehydrogenase, at 3,359,315 to 3,360,454
_RS35240
Position (kb)
3357
3358
3359 Strain fitness (log2 ratio)
-1
0
1
2 at 3356.929 kb on + strand at 3356.929 kb on + strand at 3356.930 kb on - strand at 3356.930 kb on - strand at 3356.930 kb on - strand at 3357.031 kb on - strand at 3357.315 kb on + strand, within RR42_RS35230 at 3357.315 kb on + strand, within RR42_RS35230 at 3357.315 kb on + strand, within RR42_RS35230 at 3357.315 kb on + strand, within RR42_RS35230 at 3357.315 kb on + strand, within RR42_RS35230 at 3357.316 kb on - strand, within RR42_RS35230 at 3357.316 kb on - strand, within RR42_RS35230 at 3357.316 kb on - strand, within RR42_RS35230 at 3357.316 kb on - strand, within RR42_RS35230 at 3357.316 kb on - strand, within RR42_RS35230 at 3357.603 kb on + strand, within RR42_RS35230 at 3358.022 kb on + strand, within RR42_RS35235 at 3358.223 kb on + strand, within RR42_RS35235 at 3358.358 kb on + strand, within RR42_RS35235 at 3358.469 kb on + strand, within RR42_RS35235 at 3358.859 kb on + strand at 3358.859 kb on + strand at 3358.859 kb on + strand at 3358.860 kb on - strand at 3358.860 kb on - strand at 3358.860 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.173 kb on - strand at 3359.173 kb on - strand at 3359.173 kb on - strand at 3359.400 kb on + strand at 3359.400 kb on + strand at 3359.401 kb on - strand at 3359.668 kb on - strand, within RR42_RS35240 at 3359.869 kb on + strand, within RR42_RS35240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,356,929 + -0.5 3,356,929 + -0.8 3,356,930 - -1.4 3,356,930 - -0.4 3,356,930 - -0.1 3,357,031 - +0.9 3,357,315 + RR42_RS35230 0.36 +0.0 3,357,315 + RR42_RS35230 0.36 -0.5 3,357,315 + RR42_RS35230 0.36 +0.3 3,357,315 + RR42_RS35230 0.36 +0.9 3,357,315 + RR42_RS35230 0.36 -0.3 3,357,316 - RR42_RS35230 0.36 +0.1 3,357,316 - RR42_RS35230 0.36 +0.9 3,357,316 - RR42_RS35230 0.36 +0.2 3,357,316 - RR42_RS35230 0.36 -0.1 3,357,316 - RR42_RS35230 0.36 -1.0 3,357,603 + RR42_RS35230 0.67 -0.9 3,358,022 + RR42_RS35235 0.11 -0.2 3,358,223 + RR42_RS35235 0.32 -0.7 3,358,358 + RR42_RS35235 0.46 -0.9 3,358,469 + RR42_RS35235 0.58 -0.4 3,358,859 + -0.4 3,358,859 + +1.2 3,358,859 + -0.5 3,358,860 - +0.1 3,358,860 - +1.1 3,358,860 - +0.6 3,359,160 - +0.5 3,359,160 - +0.1 3,359,160 - +0.3 3,359,160 - -0.3 3,359,160 - +0.1 3,359,173 - +1.5 3,359,173 - +0.3 3,359,173 - +1.9 3,359,400 + +0.2 3,359,400 + +0.1 3,359,401 - +0.1 3,359,668 - RR42_RS35240 0.31 -0.2 3,359,869 + RR42_RS35240 0.49 -0.7
Or see this region's nucleotide sequence