Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34585 and RR42_RS34590 are separated by 242 nucleotides RR42_RS34590 and RR42_RS34595 are separated by 58 nucleotides RR42_RS34595 and RR42_RS34600 are separated by 275 nucleotides
RR42_RS34585: RR42_RS34585 - LysR family transcriptional regulator, at 3,200,203 to 3,201,114
_RS34585
RR42_RS34590: RR42_RS34590 - glutathione S-transferase, at 3,201,357 to 3,201,971
_RS34590
RR42_RS34595: RR42_RS34595 - FAD-binding oxidoreductase, at 3,202,030 to 3,204,144
_RS34595
RR42_RS34600: RR42_RS34600 - metapyrocatechase, at 3,204,420 to 3,205,358
_RS34600
Position (kb)
3202
3203
3204
3205 Strain fitness (log2 ratio)
-1
0
1
2 at 3201.155 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.201 kb on - strand at 3201.201 kb on - strand at 3201.366 kb on + strand at 3201.366 kb on + strand at 3201.567 kb on + strand, within RR42_RS34590 at 3201.567 kb on + strand, within RR42_RS34590 at 3201.567 kb on + strand, within RR42_RS34590 at 3201.568 kb on - strand, within RR42_RS34590 at 3201.568 kb on - strand, within RR42_RS34590 at 3201.714 kb on + strand, within RR42_RS34590 at 3201.714 kb on + strand, within RR42_RS34590 at 3201.715 kb on - strand, within RR42_RS34590 at 3201.831 kb on + strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.838 kb on - strand, within RR42_RS34590 at 3201.838 kb on - strand, within RR42_RS34590 at 3202.168 kb on + strand at 3202.168 kb on + strand at 3202.169 kb on - strand at 3202.499 kb on - strand, within RR42_RS34595 at 3202.639 kb on + strand, within RR42_RS34595 at 3202.640 kb on - strand, within RR42_RS34595 at 3203.227 kb on + strand, within RR42_RS34595 at 3203.227 kb on + strand, within RR42_RS34595 at 3203.228 kb on - strand, within RR42_RS34595 at 3203.260 kb on + strand, within RR42_RS34595 at 3203.626 kb on + strand, within RR42_RS34595 at 3203.686 kb on + strand, within RR42_RS34595 at 3203.687 kb on - strand, within RR42_RS34595 at 3203.730 kb on - strand, within RR42_RS34595 at 3203.731 kb on + strand, within RR42_RS34595 at 3203.731 kb on + strand, within RR42_RS34595 at 3203.731 kb on + strand, within RR42_RS34595 at 3203.731 kb on + strand, within RR42_RS34595 at 3203.731 kb on + strand, within RR42_RS34595 at 3203.732 kb on - strand, within RR42_RS34595 at 3203.732 kb on - strand, within RR42_RS34595 at 3204.026 kb on - strand at 3204.178 kb on + strand at 3204.179 kb on - strand at 3204.273 kb on - strand at 3204.273 kb on - strand at 3204.281 kb on + strand at 3204.281 kb on + strand at 3204.281 kb on + strand at 3204.282 kb on - strand at 3204.282 kb on - strand at 3204.282 kb on - strand at 3204.285 kb on + strand at 3204.285 kb on + strand at 3204.300 kb on + strand at 3204.300 kb on + strand at 3204.300 kb on + strand at 3204.300 kb on + strand at 3204.301 kb on - strand at 3204.301 kb on - strand at 3204.301 kb on - strand at 3204.478 kb on - strand at 3204.600 kb on + strand, within RR42_RS34600 at 3204.601 kb on - strand, within RR42_RS34600 at 3204.630 kb on + strand, within RR42_RS34600 at 3204.631 kb on - strand, within RR42_RS34600 at 3204.631 kb on - strand, within RR42_RS34600 at 3204.698 kb on + strand, within RR42_RS34600 at 3204.698 kb on + strand, within RR42_RS34600 at 3204.699 kb on - strand, within RR42_RS34600 at 3204.699 kb on - strand, within RR42_RS34600 at 3204.699 kb on - strand, within RR42_RS34600 at 3204.699 kb on - strand, within RR42_RS34600 at 3204.926 kb on - strand, within RR42_RS34600 at 3204.945 kb on + strand, within RR42_RS34600 at 3204.945 kb on + strand, within RR42_RS34600 at 3205.140 kb on + strand, within RR42_RS34600 at 3205.140 kb on + strand, within RR42_RS34600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,201,155 + +0.9 3,201,200 + +0.3 3,201,200 + -0.3 3,201,200 + -0.2 3,201,200 + +0.5 3,201,201 - +0.2 3,201,201 - +1.9 3,201,366 + +0.7 3,201,366 + -0.0 3,201,567 + RR42_RS34590 0.34 -1.7 3,201,567 + RR42_RS34590 0.34 -1.0 3,201,567 + RR42_RS34590 0.34 -0.0 3,201,568 - RR42_RS34590 0.34 -0.1 3,201,568 - RR42_RS34590 0.34 -0.8 3,201,714 + RR42_RS34590 0.58 -0.6 3,201,714 + RR42_RS34590 0.58 -0.2 3,201,715 - RR42_RS34590 0.58 +0.2 3,201,831 + RR42_RS34590 0.77 +2.3 3,201,832 - RR42_RS34590 0.77 +1.2 3,201,832 - RR42_RS34590 0.77 -1.3 3,201,832 - RR42_RS34590 0.77 +0.7 3,201,832 - RR42_RS34590 0.77 +0.6 3,201,837 + RR42_RS34590 0.78 +0.7 3,201,837 + RR42_RS34590 0.78 +0.1 3,201,837 + RR42_RS34590 0.78 +0.3 3,201,838 - RR42_RS34590 0.78 -0.5 3,201,838 - RR42_RS34590 0.78 +0.3 3,202,168 + +0.8 3,202,168 + +0.6 3,202,169 - -0.5 3,202,499 - RR42_RS34595 0.22 -0.2 3,202,639 + RR42_RS34595 0.29 -0.3 3,202,640 - RR42_RS34595 0.29 +0.2 3,203,227 + RR42_RS34595 0.57 -0.4 3,203,227 + RR42_RS34595 0.57 +0.1 3,203,228 - RR42_RS34595 0.57 -0.1 3,203,260 + RR42_RS34595 0.58 -0.0 3,203,626 + RR42_RS34595 0.75 -0.4 3,203,686 + RR42_RS34595 0.78 -0.5 3,203,687 - RR42_RS34595 0.78 +0.0 3,203,730 - RR42_RS34595 0.80 -0.1 3,203,731 + RR42_RS34595 0.80 +0.8 3,203,731 + RR42_RS34595 0.80 +1.3 3,203,731 + RR42_RS34595 0.80 -0.5 3,203,731 + RR42_RS34595 0.80 +0.1 3,203,731 + RR42_RS34595 0.80 -1.1 3,203,732 - RR42_RS34595 0.80 -0.4 3,203,732 - RR42_RS34595 0.80 +0.8 3,204,026 - +1.1 3,204,178 + +0.4 3,204,179 - +0.9 3,204,273 - +0.3 3,204,273 - -0.0 3,204,281 + +0.1 3,204,281 + +1.0 3,204,281 + +0.1 3,204,282 - +0.6 3,204,282 - -0.3 3,204,282 - +0.8 3,204,285 + -0.5 3,204,285 + -0.9 3,204,300 + -0.7 3,204,300 + -0.2 3,204,300 + -0.1 3,204,300 + +0.7 3,204,301 - -0.0 3,204,301 - -0.1 3,204,301 - +1.1 3,204,478 - +1.1 3,204,600 + RR42_RS34600 0.19 -0.3 3,204,601 - RR42_RS34600 0.19 -1.1 3,204,630 + RR42_RS34600 0.22 -0.9 3,204,631 - RR42_RS34600 0.22 -0.1 3,204,631 - RR42_RS34600 0.22 +0.9 3,204,698 + RR42_RS34600 0.30 -1.0 3,204,698 + RR42_RS34600 0.30 +0.1 3,204,699 - RR42_RS34600 0.30 +0.1 3,204,699 - RR42_RS34600 0.30 -0.1 3,204,699 - RR42_RS34600 0.30 -0.2 3,204,699 - RR42_RS34600 0.30 -0.2 3,204,926 - RR42_RS34600 0.54 -0.4 3,204,945 + RR42_RS34600 0.56 +0.8 3,204,945 + RR42_RS34600 0.56 -0.4 3,205,140 + RR42_RS34600 0.77 +0.4 3,205,140 + RR42_RS34600 0.77 -0.5
Or see this region's nucleotide sequence