Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33855 and RR42_RS33860 are separated by 407 nucleotides RR42_RS33860 and RR42_RS33865 are separated by 140 nucleotides RR42_RS33865 and RR42_RS33870 are separated by 132 nucleotides
RR42_RS33855: RR42_RS33855 - TetR family transcriptional regulator, at 3,025,240 to 3,025,908
_RS33855
RR42_RS33860: RR42_RS33860 - 3-demethylubiquinone-9 3-methyltransferase, at 3,026,316 to 3,026,747
_RS33860
RR42_RS33865: RR42_RS33865 - hypothetical protein, at 3,026,888 to 3,027,088
_RS33865
RR42_RS33870: RR42_RS33870 - hypothetical protein, at 3,027,221 to 3,027,571
_RS33870
Position (kb)
3026
3027 Strain fitness (log2 ratio)
-2
-1
0
1 at 3025.392 kb on - strand, within RR42_RS33855 at 3025.392 kb on - strand, within RR42_RS33855 at 3025.470 kb on - strand, within RR42_RS33855 at 3025.583 kb on + strand, within RR42_RS33855 at 3025.583 kb on + strand, within RR42_RS33855 at 3025.868 kb on + strand at 3025.906 kb on + strand at 3025.906 kb on + strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3026.049 kb on + strand at 3026.050 kb on - strand at 3026.055 kb on - strand at 3026.067 kb on - strand at 3026.320 kb on + strand at 3026.321 kb on - strand at 3026.331 kb on + strand at 3026.331 kb on + strand at 3026.331 kb on + strand at 3026.373 kb on + strand, within RR42_RS33860 at 3026.374 kb on - strand, within RR42_RS33860 at 3026.676 kb on + strand, within RR42_RS33860 at 3026.676 kb on + strand, within RR42_RS33860 at 3027.144 kb on + strand at 3027.144 kb on + strand at 3027.144 kb on + strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.152 kb on + strand at 3027.153 kb on - strand at 3027.207 kb on + strand at 3027.208 kb on - strand at 3027.217 kb on + strand at 3027.217 kb on + strand at 3027.218 kb on - strand at 3027.218 kb on - strand at 3027.219 kb on + strand at 3027.220 kb on - strand at 3027.220 kb on - strand at 3027.453 kb on + strand, within RR42_RS33870 at 3027.453 kb on + strand, within RR42_RS33870 at 3027.454 kb on - strand, within RR42_RS33870 at 3027.476 kb on - strand, within RR42_RS33870 at 3027.476 kb on - strand, within RR42_RS33870 at 3027.565 kb on + strand at 3027.582 kb on + strand at 3027.582 kb on + strand at 3027.583 kb on - strand at 3027.583 kb on - strand at 3027.724 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.735 kb on - strand at 3027.739 kb on + strand at 3027.739 kb on + strand at 3027.739 kb on + strand at 3027.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 3,025,392 - RR42_RS33855 0.23 +0.5 3,025,392 - RR42_RS33855 0.23 +0.9 3,025,470 - RR42_RS33855 0.34 +0.3 3,025,583 + RR42_RS33855 0.51 +0.9 3,025,583 + RR42_RS33855 0.51 -0.5 3,025,868 + +0.5 3,025,906 + -0.2 3,025,906 + -1.1 3,025,907 - +0.1 3,025,907 - +0.4 3,025,907 - +0.8 3,025,907 - -0.1 3,025,907 - -0.7 3,026,049 + +0.8 3,026,050 - +1.1 3,026,055 - -1.1 3,026,067 - +0.1 3,026,320 + -0.7 3,026,321 - -0.3 3,026,331 + -0.3 3,026,331 + +1.1 3,026,331 + -0.1 3,026,373 + RR42_RS33860 0.13 -0.0 3,026,374 - RR42_RS33860 0.13 -0.9 3,026,676 + RR42_RS33860 0.83 -0.8 3,026,676 + RR42_RS33860 0.83 -1.9 3,027,144 + -0.6 3,027,144 + +0.4 3,027,144 + +0.6 3,027,145 - +0.0 3,027,145 - -0.4 3,027,145 - +0.1 3,027,145 - +0.5 3,027,152 + +0.6 3,027,153 - -0.1 3,027,207 + -0.1 3,027,208 - +0.8 3,027,217 + +0.3 3,027,217 + -0.4 3,027,218 - -0.2 3,027,218 - -0.1 3,027,219 + -0.3 3,027,220 - +0.6 3,027,220 - +0.4 3,027,453 + RR42_RS33870 0.66 -0.2 3,027,453 + RR42_RS33870 0.66 +0.0 3,027,454 - RR42_RS33870 0.66 +0.1 3,027,476 - RR42_RS33870 0.73 +0.5 3,027,476 - RR42_RS33870 0.73 -0.1 3,027,565 + +0.5 3,027,582 + -0.5 3,027,582 + -0.1 3,027,583 - +0.5 3,027,583 - +0.8 3,027,724 + +0.7 3,027,734 + +0.4 3,027,734 + -0.7 3,027,734 + +0.2 3,027,734 + -0.4 3,027,734 + -0.2 3,027,735 - -1.3 3,027,739 + -0.0 3,027,739 + +0.8 3,027,739 + -0.3 3,027,740 - +0.9
Or see this region's nucleotide sequence