Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32825 and RR42_RS32830 are separated by 220 nucleotides RR42_RS32830 and RR42_RS32835 are separated by 325 nucleotides
RR42_RS32825: RR42_RS32825 - ATPase, at 2,759,531 to 2,762,164
_RS32825
RR42_RS32830: RR42_RS32830 - hypothetical protein, at 2,762,385 to 2,762,579
_RS32830
RR42_RS32835: RR42_RS32835 - hypothetical protein, at 2,762,905 to 2,764,092
_RS32835
Position (kb)
2762
2763 Strain fitness (log2 ratio)
-2
-1
0
1 at 2761.549 kb on + strand, within RR42_RS32825 at 2761.608 kb on + strand, within RR42_RS32825 at 2761.609 kb on - strand, within RR42_RS32825 at 2761.668 kb on + strand, within RR42_RS32825 at 2761.668 kb on + strand, within RR42_RS32825 at 2761.668 kb on + strand, within RR42_RS32825 at 2761.668 kb on + strand, within RR42_RS32825 at 2761.668 kb on + strand, within RR42_RS32825 at 2761.669 kb on - strand, within RR42_RS32825 at 2761.678 kb on + strand, within RR42_RS32825 at 2761.679 kb on - strand, within RR42_RS32825 at 2761.789 kb on + strand, within RR42_RS32825 at 2761.789 kb on + strand, within RR42_RS32825 at 2761.789 kb on + strand, within RR42_RS32825 at 2761.790 kb on - strand, within RR42_RS32825 at 2761.894 kb on - strand, within RR42_RS32825 at 2761.894 kb on - strand, within RR42_RS32825 at 2761.894 kb on - strand, within RR42_RS32825 at 2761.936 kb on + strand at 2762.022 kb on + strand at 2762.040 kb on - strand at 2762.056 kb on + strand at 2762.056 kb on + strand at 2762.247 kb on + strand at 2762.247 kb on + strand at 2762.268 kb on - strand at 2762.373 kb on + strand at 2762.447 kb on + strand, within RR42_RS32830 at 2762.448 kb on - strand, within RR42_RS32830 at 2762.448 kb on - strand, within RR42_RS32830 at 2762.505 kb on + strand, within RR42_RS32830 at 2762.572 kb on - strand at 2762.572 kb on - strand at 2762.703 kb on - strand at 2762.784 kb on + strand at 2762.785 kb on - strand at 2762.785 kb on - strand at 2762.785 kb on - strand at 2762.827 kb on - strand at 2762.907 kb on - strand at 2763.214 kb on - strand, within RR42_RS32835 at 2763.367 kb on + strand, within RR42_RS32835 at 2763.463 kb on + strand, within RR42_RS32835 at 2763.521 kb on + strand, within RR42_RS32835 at 2763.522 kb on - strand, within RR42_RS32835 at 2763.522 kb on - strand, within RR42_RS32835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,761,549 + RR42_RS32825 0.77 -1.4 2,761,608 + RR42_RS32825 0.79 +0.3 2,761,609 - RR42_RS32825 0.79 +0.0 2,761,668 + RR42_RS32825 0.81 +0.4 2,761,668 + RR42_RS32825 0.81 -0.8 2,761,668 + RR42_RS32825 0.81 +0.1 2,761,668 + RR42_RS32825 0.81 -0.2 2,761,668 + RR42_RS32825 0.81 +0.5 2,761,669 - RR42_RS32825 0.81 -0.4 2,761,678 + RR42_RS32825 0.82 -0.1 2,761,679 - RR42_RS32825 0.82 +0.1 2,761,789 + RR42_RS32825 0.86 +0.4 2,761,789 + RR42_RS32825 0.86 -0.1 2,761,789 + RR42_RS32825 0.86 -0.2 2,761,790 - RR42_RS32825 0.86 -1.0 2,761,894 - RR42_RS32825 0.90 -0.3 2,761,894 - RR42_RS32825 0.90 +0.1 2,761,894 - RR42_RS32825 0.90 -0.1 2,761,936 + -0.0 2,762,022 + +0.8 2,762,040 - +0.6 2,762,056 + -0.5 2,762,056 + -0.5 2,762,247 + -0.8 2,762,247 + +0.6 2,762,268 - -0.2 2,762,373 + +1.0 2,762,447 + RR42_RS32830 0.32 -1.5 2,762,448 - RR42_RS32830 0.32 -0.6 2,762,448 - RR42_RS32830 0.32 +0.7 2,762,505 + RR42_RS32830 0.62 -2.0 2,762,572 - +0.1 2,762,572 - +0.7 2,762,703 - +1.6 2,762,784 + +0.3 2,762,785 - +0.0 2,762,785 - -0.3 2,762,785 - -0.5 2,762,827 - -1.1 2,762,907 - -0.5 2,763,214 - RR42_RS32835 0.26 +0.6 2,763,367 + RR42_RS32835 0.39 -1.2 2,763,463 + RR42_RS32835 0.47 +0.0 2,763,521 + RR42_RS32835 0.52 +0.1 2,763,522 - RR42_RS32835 0.52 +1.6 2,763,522 - RR42_RS32835 0.52 +0.7
Or see this region's nucleotide sequence