Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32635 and RR42_RS32640 are separated by 38 nucleotides RR42_RS32640 and RR42_RS32645 are separated by 32 nucleotides RR42_RS32645 and RR42_RS32650 are separated by 8 nucleotides
RR42_RS32635: RR42_RS32635 - 4-oxalocrotonate tautomerase, at 2,719,552 to 2,719,743
_RS32635
RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570
_RS32640
RR42_RS32645: RR42_RS32645 - 2-keto-4-pentenoate hydratase, at 2,720,603 to 2,721,385
_RS32645
RR42_RS32650: RR42_RS32650 - betaine-aldehyde dehydrogenase, at 2,721,394 to 2,722,848
_RS32650
Position (kb)
2720
2721
2722 Strain fitness (log2 ratio)
-2
-1
0
1 at 2720.589 kb on + strand at 2721.265 kb on + strand, within RR42_RS32645 at 2721.271 kb on - strand, within RR42_RS32645 at 2721.371 kb on - strand at 2721.428 kb on + strand at 2721.428 kb on + strand at 2721.663 kb on + strand, within RR42_RS32650 at 2721.830 kb on + strand, within RR42_RS32650 at 2721.972 kb on - strand, within RR42_RS32650 at 2721.972 kb on - strand, within RR42_RS32650 at 2722.111 kb on + strand, within RR42_RS32650 at 2722.149 kb on + strand, within RR42_RS32650 at 2722.244 kb on + strand, within RR42_RS32650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,720,589 + +0.4 2,721,265 + RR42_RS32645 0.85 -2.1 2,721,271 - RR42_RS32645 0.85 -1.0 2,721,371 - +0.0 2,721,428 + -0.6 2,721,428 + +0.2 2,721,663 + RR42_RS32650 0.18 +0.6 2,721,830 + RR42_RS32650 0.30 -0.5 2,721,972 - RR42_RS32650 0.40 -0.1 2,721,972 - RR42_RS32650 0.40 -0.0 2,722,111 + RR42_RS32650 0.49 +0.3 2,722,149 + RR42_RS32650 0.52 +0.2 2,722,244 + RR42_RS32650 0.58 -1.3
Or see this region's nucleotide sequence