Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32615 and RR42_RS32620 are separated by 531 nucleotides RR42_RS32620 and RR42_RS32625 are separated by 100 nucleotides
RR42_RS32615: RR42_RS32615 - methionine ABC transporter ATPase, at 2,714,260 to 2,715,072
_RS32615
RR42_RS32620: RR42_RS32620 - chemotaxis protein, at 2,715,604 to 2,717,412
_RS32620
RR42_RS32625: RR42_RS32625 - 4-hyroxy-2-oxovalerate aldolase, at 2,717,513 to 2,718,562
_RS32625
Position (kb)
2715
2716
2717
2718 Strain fitness (log2 ratio)
-1
0
1 at 2714.605 kb on + strand, within RR42_RS32615 at 2714.605 kb on + strand, within RR42_RS32615 at 2714.606 kb on - strand, within RR42_RS32615 at 2714.606 kb on - strand, within RR42_RS32615 at 2714.927 kb on - strand, within RR42_RS32615 at 2714.927 kb on - strand, within RR42_RS32615 at 2714.929 kb on + strand, within RR42_RS32615 at 2714.929 kb on + strand, within RR42_RS32615 at 2714.929 kb on + strand, within RR42_RS32615 at 2714.930 kb on - strand, within RR42_RS32615 at 2714.930 kb on - strand, within RR42_RS32615 at 2714.930 kb on - strand, within RR42_RS32615 at 2714.998 kb on + strand at 2714.998 kb on + strand at 2715.062 kb on - strand at 2715.157 kb on + strand at 2715.306 kb on + strand at 2715.307 kb on - strand at 2715.526 kb on + strand at 2715.527 kb on - strand at 2715.603 kb on + strand at 2715.603 kb on + strand at 2715.603 kb on + strand at 2715.603 kb on + strand at 2715.604 kb on - strand at 2715.604 kb on - strand at 2715.604 kb on - strand at 2715.895 kb on + strand, within RR42_RS32620 at 2715.895 kb on + strand, within RR42_RS32620 at 2715.980 kb on - strand, within RR42_RS32620 at 2715.980 kb on - strand, within RR42_RS32620 at 2715.980 kb on - strand, within RR42_RS32620 at 2716.024 kb on + strand, within RR42_RS32620 at 2716.024 kb on + strand, within RR42_RS32620 at 2716.025 kb on - strand, within RR42_RS32620 at 2716.025 kb on - strand, within RR42_RS32620 at 2716.221 kb on + strand, within RR42_RS32620 at 2716.221 kb on + strand, within RR42_RS32620 at 2716.685 kb on + strand, within RR42_RS32620 at 2716.685 kb on + strand, within RR42_RS32620 at 2716.685 kb on + strand, within RR42_RS32620 at 2717.477 kb on + strand at 2717.478 kb on - strand at 2717.612 kb on + strand at 2717.646 kb on + strand, within RR42_RS32625 at 2717.688 kb on + strand, within RR42_RS32625 at 2717.788 kb on - strand, within RR42_RS32625 at 2718.032 kb on - strand, within RR42_RS32625 at 2718.169 kb on + strand, within RR42_RS32625 at 2718.170 kb on - strand, within RR42_RS32625 at 2718.305 kb on + strand, within RR42_RS32625 at 2718.376 kb on - strand, within RR42_RS32625 at 2718.380 kb on - strand, within RR42_RS32625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,714,605 + RR42_RS32615 0.42 -0.5 2,714,605 + RR42_RS32615 0.42 -0.8 2,714,606 - RR42_RS32615 0.43 -0.1 2,714,606 - RR42_RS32615 0.43 +0.1 2,714,927 - RR42_RS32615 0.82 +0.0 2,714,927 - RR42_RS32615 0.82 -1.4 2,714,929 + RR42_RS32615 0.82 +0.5 2,714,929 + RR42_RS32615 0.82 -0.3 2,714,929 + RR42_RS32615 0.82 +0.0 2,714,930 - RR42_RS32615 0.82 -0.3 2,714,930 - RR42_RS32615 0.82 +0.8 2,714,930 - RR42_RS32615 0.82 +0.7 2,714,998 + -0.2 2,714,998 + -0.0 2,715,062 - -0.6 2,715,157 + +1.0 2,715,306 + +0.1 2,715,307 - +1.0 2,715,526 + -1.5 2,715,527 - -0.2 2,715,603 + -0.2 2,715,603 + -0.1 2,715,603 + -0.0 2,715,603 + +0.5 2,715,604 - +0.6 2,715,604 - -0.2 2,715,604 - +0.6 2,715,895 + RR42_RS32620 0.16 -0.2 2,715,895 + RR42_RS32620 0.16 -0.6 2,715,980 - RR42_RS32620 0.21 -0.3 2,715,980 - RR42_RS32620 0.21 +0.2 2,715,980 - RR42_RS32620 0.21 -0.3 2,716,024 + RR42_RS32620 0.23 -1.3 2,716,024 + RR42_RS32620 0.23 +0.5 2,716,025 - RR42_RS32620 0.23 +0.4 2,716,025 - RR42_RS32620 0.23 -0.3 2,716,221 + RR42_RS32620 0.34 -0.7 2,716,221 + RR42_RS32620 0.34 -0.4 2,716,685 + RR42_RS32620 0.60 -0.3 2,716,685 + RR42_RS32620 0.60 -0.3 2,716,685 + RR42_RS32620 0.60 -0.9 2,717,477 + -0.1 2,717,478 - +0.1 2,717,612 + -0.4 2,717,646 + RR42_RS32625 0.13 -0.8 2,717,688 + RR42_RS32625 0.17 -0.2 2,717,788 - RR42_RS32625 0.26 +0.0 2,718,032 - RR42_RS32625 0.49 -1.2 2,718,169 + RR42_RS32625 0.62 +0.6 2,718,170 - RR42_RS32625 0.63 +0.4 2,718,305 + RR42_RS32625 0.75 -0.5 2,718,376 - RR42_RS32625 0.82 -0.7 2,718,380 - RR42_RS32625 0.83 -1.1
Or see this region's nucleotide sequence