Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32110 and RR42_RS32115 are separated by 100 nucleotides RR42_RS32115 and RR42_RS32120 are separated by 57 nucleotides RR42_RS32120 and RR42_RS32125 overlap by 4 nucleotides
RR42_RS32110: RR42_RS32110 - peptide ABC transporter permease, at 2,588,071 to 2,589,822
_RS32110
RR42_RS32115: RR42_RS32115 - curli production assembly protein CsgG, at 2,589,923 to 2,590,603
_RS32115
RR42_RS32120: RR42_RS32120 - lipoprotein, at 2,590,661 to 2,591,008
_RS32120
RR42_RS32125: RR42_RS32125 - lipoprotein, at 2,591,005 to 2,591,664
_RS32125
Position (kb)
2589
2590
2591 Strain fitness (log2 ratio)
-2
-1
0
1 at 2588.972 kb on - strand, within RR42_RS32110 at 2588.972 kb on - strand, within RR42_RS32110 at 2589.143 kb on - strand, within RR42_RS32110 at 2589.251 kb on - strand, within RR42_RS32110 at 2589.479 kb on - strand, within RR42_RS32110 at 2589.820 kb on + strand at 2589.821 kb on - strand at 2589.892 kb on + strand at 2589.892 kb on + strand at 2589.893 kb on - strand at 2589.902 kb on + strand at 2589.902 kb on + strand at 2589.903 kb on - strand at 2589.949 kb on - strand at 2590.046 kb on + strand, within RR42_RS32115 at 2590.047 kb on - strand, within RR42_RS32115 at 2590.050 kb on + strand, within RR42_RS32115 at 2590.051 kb on - strand, within RR42_RS32115 at 2590.100 kb on + strand, within RR42_RS32115 at 2590.100 kb on + strand, within RR42_RS32115 at 2590.101 kb on - strand, within RR42_RS32115 at 2590.101 kb on - strand, within RR42_RS32115 at 2590.102 kb on + strand, within RR42_RS32115 at 2590.102 kb on + strand, within RR42_RS32115 at 2590.103 kb on - strand, within RR42_RS32115 at 2590.277 kb on + strand, within RR42_RS32115 at 2590.278 kb on - strand, within RR42_RS32115 at 2590.278 kb on - strand, within RR42_RS32115 at 2590.374 kb on - strand, within RR42_RS32115 at 2590.374 kb on - strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.496 kb on + strand, within RR42_RS32115 at 2590.497 kb on - strand, within RR42_RS32115 at 2590.497 kb on - strand, within RR42_RS32115 at 2590.693 kb on - strand at 2590.693 kb on - strand at 2590.727 kb on + strand, within RR42_RS32120 at 2590.742 kb on + strand, within RR42_RS32120 at 2590.743 kb on - strand, within RR42_RS32120 at 2590.763 kb on + strand, within RR42_RS32120 at 2590.764 kb on - strand, within RR42_RS32120 at 2590.841 kb on - strand, within RR42_RS32120 at 2590.880 kb on + strand, within RR42_RS32120 at 2590.959 kb on - strand, within RR42_RS32120 at 2591.173 kb on + strand, within RR42_RS32125 at 2591.174 kb on - strand, within RR42_RS32125 at 2591.174 kb on - strand, within RR42_RS32125 at 2591.174 kb on - strand, within RR42_RS32125 at 2591.174 kb on - strand, within RR42_RS32125 at 2591.174 kb on - strand, within RR42_RS32125 at 2591.198 kb on - strand, within RR42_RS32125 at 2591.221 kb on + strand, within RR42_RS32125 at 2591.221 kb on + strand, within RR42_RS32125 at 2591.222 kb on - strand, within RR42_RS32125 at 2591.222 kb on - strand, within RR42_RS32125 at 2591.286 kb on + strand, within RR42_RS32125 at 2591.288 kb on + strand, within RR42_RS32125 at 2591.344 kb on + strand, within RR42_RS32125 at 2591.375 kb on - strand, within RR42_RS32125 at 2591.375 kb on - strand, within RR42_RS32125 at 2591.566 kb on + strand, within RR42_RS32125 at 2591.566 kb on + strand, within RR42_RS32125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,588,972 - RR42_RS32110 0.51 -1.2 2,588,972 - RR42_RS32110 0.51 -0.6 2,589,143 - RR42_RS32110 0.61 -1.3 2,589,251 - RR42_RS32110 0.67 +0.0 2,589,479 - RR42_RS32110 0.80 -1.2 2,589,820 + -0.5 2,589,821 - +0.4 2,589,892 + -0.9 2,589,892 + +1.0 2,589,893 - -0.0 2,589,902 + +0.3 2,589,902 + +0.0 2,589,903 - +1.0 2,589,949 - -1.3 2,590,046 + RR42_RS32115 0.18 +0.5 2,590,047 - RR42_RS32115 0.18 -1.1 2,590,050 + RR42_RS32115 0.19 +0.2 2,590,051 - RR42_RS32115 0.19 +0.9 2,590,100 + RR42_RS32115 0.26 +0.1 2,590,100 + RR42_RS32115 0.26 +0.9 2,590,101 - RR42_RS32115 0.26 -0.1 2,590,101 - RR42_RS32115 0.26 -0.3 2,590,102 + RR42_RS32115 0.26 -0.2 2,590,102 + RR42_RS32115 0.26 +0.7 2,590,103 - RR42_RS32115 0.26 +0.6 2,590,277 + RR42_RS32115 0.52 +0.1 2,590,278 - RR42_RS32115 0.52 +0.0 2,590,278 - RR42_RS32115 0.52 +0.6 2,590,374 - RR42_RS32115 0.66 +0.7 2,590,374 - RR42_RS32115 0.66 +0.1 2,590,473 + RR42_RS32115 0.81 +0.7 2,590,473 + RR42_RS32115 0.81 -0.0 2,590,473 + RR42_RS32115 0.81 +1.0 2,590,496 + RR42_RS32115 0.84 +0.4 2,590,497 - RR42_RS32115 0.84 +1.1 2,590,497 - RR42_RS32115 0.84 -1.6 2,590,693 - -0.0 2,590,693 - -0.5 2,590,727 + RR42_RS32120 0.19 -0.8 2,590,742 + RR42_RS32120 0.23 -0.5 2,590,743 - RR42_RS32120 0.24 +0.5 2,590,763 + RR42_RS32120 0.29 +0.7 2,590,764 - RR42_RS32120 0.30 -1.1 2,590,841 - RR42_RS32120 0.52 -0.2 2,590,880 + RR42_RS32120 0.63 -0.5 2,590,959 - RR42_RS32120 0.86 -1.1 2,591,173 + RR42_RS32125 0.25 +0.7 2,591,174 - RR42_RS32125 0.26 +1.0 2,591,174 - RR42_RS32125 0.26 -0.4 2,591,174 - RR42_RS32125 0.26 +0.7 2,591,174 - RR42_RS32125 0.26 -0.2 2,591,174 - RR42_RS32125 0.26 +0.4 2,591,198 - RR42_RS32125 0.29 -0.4 2,591,221 + RR42_RS32125 0.33 -1.8 2,591,221 + RR42_RS32125 0.33 -0.6 2,591,222 - RR42_RS32125 0.33 -0.7 2,591,222 - RR42_RS32125 0.33 -0.4 2,591,286 + RR42_RS32125 0.43 -0.2 2,591,288 + RR42_RS32125 0.43 -1.0 2,591,344 + RR42_RS32125 0.51 +0.3 2,591,375 - RR42_RS32125 0.56 -0.7 2,591,375 - RR42_RS32125 0.56 -0.4 2,591,566 + RR42_RS32125 0.85 -0.1 2,591,566 + RR42_RS32125 0.85 +0.1
Or see this region's nucleotide sequence