Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31345 and RR42_RS31350 are separated by 144 nucleotides RR42_RS31350 and RR42_RS31355 are separated by 76 nucleotides RR42_RS31355 and RR42_RS31360 are separated by 118 nucleotides
RR42_RS31345: RR42_RS31345 - LysR family transcriptional regulator, at 2,408,077 to 2,408,388
_RS31345
RR42_RS31350: RR42_RS31350 - hypothetical protein, at 2,408,533 to 2,409,087
_RS31350
RR42_RS31355: RR42_RS31355 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,409,164 to 2,409,937
_RS31355
RR42_RS31360: RR42_RS31360 - CoA-binding protein, at 2,410,056 to 2,412,185
_RS31360
Position (kb)
2409
2410 Strain fitness (log2 ratio)
-2
-1
0
1 at 2408.170 kb on + strand, within RR42_RS31345 at 2408.170 kb on + strand, within RR42_RS31345 at 2408.171 kb on - strand, within RR42_RS31345 at 2408.224 kb on + strand, within RR42_RS31345 at 2408.224 kb on + strand, within RR42_RS31345 at 2408.224 kb on + strand, within RR42_RS31345 at 2408.225 kb on - strand, within RR42_RS31345 at 2408.225 kb on - strand, within RR42_RS31345 at 2408.285 kb on - strand, within RR42_RS31345 at 2408.308 kb on + strand, within RR42_RS31345 at 2408.308 kb on + strand, within RR42_RS31345 at 2408.308 kb on + strand, within RR42_RS31345 at 2408.309 kb on - strand, within RR42_RS31345 at 2408.356 kb on + strand, within RR42_RS31345 at 2408.356 kb on + strand, within RR42_RS31345 at 2408.481 kb on + strand at 2408.492 kb on + strand at 2408.493 kb on - strand at 2408.746 kb on + strand, within RR42_RS31350 at 2408.746 kb on + strand, within RR42_RS31350 at 2408.747 kb on - strand, within RR42_RS31350 at 2408.750 kb on - strand, within RR42_RS31350 at 2408.887 kb on + strand, within RR42_RS31350 at 2408.887 kb on + strand, within RR42_RS31350 at 2408.887 kb on + strand, within RR42_RS31350 at 2408.887 kb on + strand, within RR42_RS31350 at 2409.159 kb on - strand at 2409.351 kb on + strand, within RR42_RS31355 at 2409.531 kb on + strand, within RR42_RS31355 at 2409.541 kb on + strand, within RR42_RS31355 at 2409.541 kb on + strand, within RR42_RS31355 at 2409.542 kb on - strand, within RR42_RS31355 at 2409.631 kb on - strand, within RR42_RS31355 at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.020 kb on - strand at 2410.020 kb on - strand at 2410.020 kb on - strand at 2410.038 kb on + strand at 2410.088 kb on - strand at 2410.088 kb on - strand at 2410.154 kb on - strand at 2410.154 kb on - strand at 2410.159 kb on + strand at 2410.160 kb on - strand at 2410.160 kb on - strand at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.290 kb on - strand, within RR42_RS31360 at 2410.300 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.389 kb on - strand, within RR42_RS31360 at 2410.389 kb on - strand, within RR42_RS31360 at 2410.649 kb on - strand, within RR42_RS31360 at 2410.649 kb on - strand, within RR42_RS31360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,408,170 + RR42_RS31345 0.30 -0.8 2,408,170 + RR42_RS31345 0.30 -0.1 2,408,171 - RR42_RS31345 0.30 -0.0 2,408,224 + RR42_RS31345 0.47 -0.4 2,408,224 + RR42_RS31345 0.47 +1.2 2,408,224 + RR42_RS31345 0.47 +0.9 2,408,225 - RR42_RS31345 0.47 +0.8 2,408,225 - RR42_RS31345 0.47 +0.0 2,408,285 - RR42_RS31345 0.67 +0.2 2,408,308 + RR42_RS31345 0.74 -0.2 2,408,308 + RR42_RS31345 0.74 +0.5 2,408,308 + RR42_RS31345 0.74 +0.8 2,408,309 - RR42_RS31345 0.74 +0.2 2,408,356 + RR42_RS31345 0.89 +0.9 2,408,356 + RR42_RS31345 0.89 +0.0 2,408,481 + +0.2 2,408,492 + -1.9 2,408,493 - +0.3 2,408,746 + RR42_RS31350 0.38 -0.1 2,408,746 + RR42_RS31350 0.38 +0.2 2,408,747 - RR42_RS31350 0.39 +0.6 2,408,750 - RR42_RS31350 0.39 -0.3 2,408,887 + RR42_RS31350 0.64 -0.1 2,408,887 + RR42_RS31350 0.64 +0.1 2,408,887 + RR42_RS31350 0.64 +0.1 2,408,887 + RR42_RS31350 0.64 +0.1 2,409,159 - +0.6 2,409,351 + RR42_RS31355 0.24 -0.9 2,409,531 + RR42_RS31355 0.47 -1.9 2,409,541 + RR42_RS31355 0.49 -0.5 2,409,541 + RR42_RS31355 0.49 -0.1 2,409,542 - RR42_RS31355 0.49 -0.2 2,409,631 - RR42_RS31355 0.60 -2.0 2,410,019 + +0.1 2,410,019 + +0.5 2,410,019 + -0.5 2,410,019 + +0.2 2,410,019 + -0.1 2,410,019 + -0.4 2,410,020 - -0.2 2,410,020 - +0.7 2,410,020 - -0.7 2,410,038 + -0.5 2,410,088 - -0.4 2,410,088 - -0.2 2,410,154 - -0.4 2,410,154 - +0.1 2,410,159 + +0.4 2,410,160 - +0.2 2,410,160 - +0.3 2,410,289 + RR42_RS31360 0.11 +0.3 2,410,289 + RR42_RS31360 0.11 +0.3 2,410,289 + RR42_RS31360 0.11 +0.9 2,410,289 + RR42_RS31360 0.11 -1.4 2,410,289 + RR42_RS31360 0.11 +0.1 2,410,290 - RR42_RS31360 0.11 -0.6 2,410,300 + RR42_RS31360 0.11 -0.4 2,410,360 + RR42_RS31360 0.14 -0.1 2,410,360 + RR42_RS31360 0.14 +0.7 2,410,360 + RR42_RS31360 0.14 +0.6 2,410,389 - RR42_RS31360 0.16 -0.2 2,410,389 - RR42_RS31360 0.16 -1.1 2,410,649 - RR42_RS31360 0.28 +0.7 2,410,649 - RR42_RS31360 0.28 +1.0
Or see this region's nucleotide sequence