Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30925 and RR42_RS30930 are separated by 192 nucleotides RR42_RS30930 and RR42_RS30935 are separated by 171 nucleotides RR42_RS30935 and RR42_RS30940 are separated by 74 nucleotides
RR42_RS30925: RR42_RS30925 - chitin deacetylase, at 2,319,261 to 2,320,151
_RS30925
RR42_RS30930: RR42_RS30930 - 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase, at 2,320,344 to 2,322,734
_RS30930
RR42_RS30935: RR42_RS30935 - hypothetical protein, at 2,322,906 to 2,323,310
_RS30935
RR42_RS30940: RR42_RS30940 - cobalt-zinc-cadmium resistance protein, at 2,323,385 to 2,324,653
_RS30940
Position (kb)
2320
2321
2322
2323 Strain fitness (log2 ratio)
-2
-1
0
1 at 2320.150 kb on - strand at 2320.213 kb on - strand at 2320.349 kb on + strand at 2320.350 kb on - strand at 2320.350 kb on - strand at 2320.350 kb on - strand at 2320.710 kb on - strand, within RR42_RS30930 at 2320.710 kb on - strand, within RR42_RS30930 at 2320.710 kb on - strand, within RR42_RS30930 at 2321.019 kb on - strand, within RR42_RS30930 at 2321.078 kb on - strand, within RR42_RS30930 at 2321.080 kb on + strand, within RR42_RS30930 at 2321.081 kb on - strand, within RR42_RS30930 at 2321.375 kb on - strand, within RR42_RS30930 at 2321.375 kb on - strand, within RR42_RS30930 at 2321.581 kb on + strand, within RR42_RS30930 at 2321.582 kb on - strand, within RR42_RS30930 at 2321.817 kb on + strand, within RR42_RS30930 at 2321.818 kb on - strand, within RR42_RS30930 at 2322.140 kb on - strand, within RR42_RS30930 at 2322.156 kb on + strand, within RR42_RS30930 at 2322.381 kb on - strand, within RR42_RS30930 at 2322.503 kb on - strand at 2322.647 kb on + strand at 2322.647 kb on + strand at 2322.647 kb on + strand at 2322.647 kb on + strand at 2322.823 kb on - strand at 2322.856 kb on + strand at 2322.892 kb on - strand at 2322.892 kb on - strand at 2322.892 kb on - strand at 2322.893 kb on + strand at 2322.893 kb on + strand at 2322.893 kb on + strand at 2322.893 kb on + strand at 2322.894 kb on - strand at 2322.894 kb on - strand at 2322.896 kb on - strand at 2322.896 kb on - strand at 2322.896 kb on - strand at 2322.896 kb on - strand at 2323.052 kb on + strand, within RR42_RS30935 at 2323.053 kb on - strand, within RR42_RS30935 at 2323.233 kb on + strand, within RR42_RS30935 at 2323.254 kb on + strand, within RR42_RS30935 at 2323.254 kb on + strand, within RR42_RS30935 at 2323.255 kb on - strand, within RR42_RS30935 at 2323.255 kb on - strand, within RR42_RS30935 at 2323.330 kb on + strand at 2323.684 kb on + strand, within RR42_RS30940 at 2323.684 kb on + strand, within RR42_RS30940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,320,150 - +1.0 2,320,213 - +0.5 2,320,349 + +0.2 2,320,350 - +0.2 2,320,350 - -1.8 2,320,350 - -1.0 2,320,710 - RR42_RS30930 0.15 -0.6 2,320,710 - RR42_RS30930 0.15 +0.4 2,320,710 - RR42_RS30930 0.15 -0.1 2,321,019 - RR42_RS30930 0.28 -1.4 2,321,078 - RR42_RS30930 0.31 +0.8 2,321,080 + RR42_RS30930 0.31 -1.8 2,321,081 - RR42_RS30930 0.31 -0.7 2,321,375 - RR42_RS30930 0.43 -1.9 2,321,375 - RR42_RS30930 0.43 -0.9 2,321,581 + RR42_RS30930 0.52 -0.1 2,321,582 - RR42_RS30930 0.52 +0.1 2,321,817 + RR42_RS30930 0.62 -1.2 2,321,818 - RR42_RS30930 0.62 -1.9 2,322,140 - RR42_RS30930 0.75 -1.7 2,322,156 + RR42_RS30930 0.76 -2.1 2,322,381 - RR42_RS30930 0.85 -2.3 2,322,503 - -1.2 2,322,647 + +0.4 2,322,647 + -0.3 2,322,647 + -0.7 2,322,647 + -2.7 2,322,823 - +0.7 2,322,856 + +0.1 2,322,892 - +1.5 2,322,892 - +0.8 2,322,892 - -0.2 2,322,893 + +0.6 2,322,893 + -0.0 2,322,893 + -0.2 2,322,893 + +0.1 2,322,894 - +0.5 2,322,894 - +0.7 2,322,896 - -1.1 2,322,896 - +0.2 2,322,896 - -0.3 2,322,896 - -2.5 2,323,052 + RR42_RS30935 0.36 +1.1 2,323,053 - RR42_RS30935 0.36 -0.4 2,323,233 + RR42_RS30935 0.81 -0.1 2,323,254 + RR42_RS30935 0.86 -0.4 2,323,254 + RR42_RS30935 0.86 +0.4 2,323,255 - RR42_RS30935 0.86 -0.3 2,323,255 - RR42_RS30935 0.86 -0.3 2,323,330 + +0.3 2,323,684 + RR42_RS30940 0.24 +0.2 2,323,684 + RR42_RS30940 0.24 +0.1
Or see this region's nucleotide sequence