Experiment: R2A with D-Cycloserine 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30830 and RR42_RS30835 are separated by 167 nucleotides RR42_RS30835 and RR42_RS30840 are separated by 67 nucleotides
RR42_RS30830: RR42_RS30830 - GTP cyclohydrolase, at 2,294,984 to 2,296,042
_RS30830
RR42_RS30835: RR42_RS30835 - hypothetical protein, at 2,296,210 to 2,296,686
_RS30835
RR42_RS30840: RR42_RS30840 - galactosyldiacylglycerol synthase, at 2,296,754 to 2,297,935
_RS30840
Position (kb)
2296
2297 Strain fitness (log2 ratio)
-1
0
1 at 2295.411 kb on + strand, within RR42_RS30830 at 2295.411 kb on + strand, within RR42_RS30830 at 2295.424 kb on + strand, within RR42_RS30830 at 2295.425 kb on - strand, within RR42_RS30830 at 2295.591 kb on + strand, within RR42_RS30830 at 2295.591 kb on + strand, within RR42_RS30830 at 2295.592 kb on - strand, within RR42_RS30830 at 2295.743 kb on - strand, within RR42_RS30830 at 2295.754 kb on - strand, within RR42_RS30830 at 2295.754 kb on - strand, within RR42_RS30830 at 2295.891 kb on + strand, within RR42_RS30830 at 2295.891 kb on + strand, within RR42_RS30830 at 2295.891 kb on + strand, within RR42_RS30830 at 2295.954 kb on + strand at 2295.954 kb on + strand at 2295.954 kb on + strand at 2295.954 kb on + strand at 2295.955 kb on - strand at 2296.050 kb on - strand at 2296.050 kb on - strand at 2296.065 kb on + strand at 2296.065 kb on + strand at 2296.065 kb on + strand at 2296.065 kb on + strand at 2296.084 kb on - strand at 2296.176 kb on + strand at 2296.176 kb on + strand at 2296.182 kb on + strand at 2296.183 kb on - strand at 2296.195 kb on - strand at 2296.207 kb on + strand at 2296.207 kb on + strand at 2296.208 kb on - strand at 2296.208 kb on - strand at 2296.208 kb on - strand at 2296.289 kb on + strand, within RR42_RS30835 at 2296.504 kb on + strand, within RR42_RS30835 at 2296.505 kb on - strand, within RR42_RS30835 at 2296.548 kb on + strand, within RR42_RS30835 at 2296.548 kb on + strand, within RR42_RS30835 at 2296.549 kb on - strand, within RR42_RS30835 at 2296.643 kb on + strand at 2296.644 kb on - strand at 2296.644 kb on - strand at 2296.671 kb on + strand at 2296.672 kb on - strand at 2296.704 kb on - strand at 2297.129 kb on - strand, within RR42_RS30840 at 2297.297 kb on + strand, within RR42_RS30840 at 2297.298 kb on - strand, within RR42_RS30840 at 2297.298 kb on - strand, within RR42_RS30840 at 2297.466 kb on + strand, within RR42_RS30840
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.01 mg/ml remove 2,295,411 + RR42_RS30830 0.40 -1.1 2,295,411 + RR42_RS30830 0.40 +0.1 2,295,424 + RR42_RS30830 0.42 -0.2 2,295,425 - RR42_RS30830 0.42 +0.2 2,295,591 + RR42_RS30830 0.57 -0.4 2,295,591 + RR42_RS30830 0.57 +0.4 2,295,592 - RR42_RS30830 0.57 -0.6 2,295,743 - RR42_RS30830 0.72 +0.1 2,295,754 - RR42_RS30830 0.73 +0.6 2,295,754 - RR42_RS30830 0.73 -1.4 2,295,891 + RR42_RS30830 0.86 -0.8 2,295,891 + RR42_RS30830 0.86 +0.4 2,295,891 + RR42_RS30830 0.86 -0.2 2,295,954 + -0.7 2,295,954 + -0.5 2,295,954 + -0.1 2,295,954 + -0.0 2,295,955 - -1.1 2,296,050 - +0.7 2,296,050 - +0.0 2,296,065 + -0.4 2,296,065 + +0.3 2,296,065 + +0.0 2,296,065 + -0.3 2,296,084 - +0.1 2,296,176 + -0.0 2,296,176 + -0.8 2,296,182 + -1.0 2,296,183 - +0.9 2,296,195 - -0.4 2,296,207 + -0.0 2,296,207 + +0.8 2,296,208 - -0.2 2,296,208 - -1.1 2,296,208 - -0.0 2,296,289 + RR42_RS30835 0.17 -0.2 2,296,504 + RR42_RS30835 0.62 -0.3 2,296,505 - RR42_RS30835 0.62 -1.7 2,296,548 + RR42_RS30835 0.71 -0.4 2,296,548 + RR42_RS30835 0.71 -0.9 2,296,549 - RR42_RS30835 0.71 -0.9 2,296,643 + +0.3 2,296,644 - +0.1 2,296,644 - +0.2 2,296,671 + +0.7 2,296,672 - +0.1 2,296,704 - +0.4 2,297,129 - RR42_RS30840 0.32 +0.1 2,297,297 + RR42_RS30840 0.46 +0.7 2,297,298 - RR42_RS30840 0.46 +0.3 2,297,298 - RR42_RS30840 0.46 +0.2 2,297,466 + RR42_RS30840 0.60 -1.1
Or see this region's nucleotide sequence